Issue · 2014
Vol.11, Iss. 2
27 articles · All issues →
ArticlesMolecular and Biological Characterization of Squash Leaf Curl Virus (SLCV) Affecting Common Beans in Egypt
Amro A. Farrag, Ahmed K. El-Attar, Om-Hashem M. El-Banna, Ibrahim A. I, H. M. Mazyad
Symptomatic leaf samples obtained from common bean plants were collected from Qaliobeya governorate, Egypt, and tested for Squash Leaf Curl Virus (SLCV) infection by PCR using both degenerate and specific oligonucleotide primers. SLCV (bean isolate) was transm…
16 Aug 2021- Articles
Effect of Gamma Irradiation on Production of Phytoplasma-Free Hibiscus Plantlets
Samah A. Mokbel, Ahmed K. El-Attar
Controlling phytoplasma-causing disease through combination of tissue culture technique and radiation treatment represented a short method for high level management. To investigate the effect of gamma rays to produce healthy plantlets, the hibiscus witches' br…
16 Aug 2021 - Articles
Characterizations of the Egyptian isolate of Onion yellow dwarf virus Infecting onion and development of Virus-Free plantlets
Ahmed K. El-Attar, Samah A. Mokbel, Ali H. Hamed
Onion yellow dwarf disease is an economically important disease of onion (Allium cepa L.) caused by Onion yellow dwarf virus (OYDV). In this research we aimed to identify and molecularly characterize the isolate of the OYDV infecting onion plants in Egypt and…
16 Aug 2021 - Articles
Anatomical and Ultrastructural Changes in Tomato and Grapevine Leaf Tissues Infected with Tomato ringspot virus
Om-Hashem M. El-Banna, Maisa A.E. Awad, M.S. Abbas, Hoda M. A. Waziri, Huda S. A. Darwish
Tomato ringspot virus (ToRSV) is one of the most serious viruses affecting grapevine, tomato and other hosts. The virus was isolated from infected grapevine which was characterized by ring spots, mottling and shortening of internodes. And from tomato exhibitin…
16 Aug 2021 - Articles
Characterization and molecular studies of Bean common mosaic virus isolated from bean plants in Egypt
M. A. S. El-kady, A. B. Badr, Ahmed K. El-Attar, Hoda M. A. Waziri, Kh. E. A. Saker
1Virus and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt. 2Department of Botany, Al-Azhar University, Cairo, Egypt.
16 Aug 2021 - Articles
The Detection and Diversity of Banana Streak Virus Isolates in Egypt
Aly M. Abdel-Salam, Rehab A. Dawoud, Amira M.E.Aly, Salama M. El-Saghir
Banana plants from Williams' (AAA, Cavendish subgroup) banana (Musa acuminate) variety and from Pradica (AAB group) banana (Musa × paradisiaca) variety, collected from different locations in Egypt, were tested for the presence of Banana streak virus (BSV…
16 Aug 2021 - Articles
Anatomical and cytological changes in bean leaf cells infected with Bean common mosaic virus
A. B. Badr, M. A. S. El-kady, Kh. E. A. Saker
Bean common mosaic virus (BCMV) infection has bean shown to influence the anatomical and cytological characters of infected bean plants. The thickness of leaflet blades, palisade and spongy tissues were increased than the healthy plants. In contrast the thickn…
16 Aug 2021 - Articles
The occurrence of Sugarcane bacilliform virus on sugarcane in Egypt
Aly M. Abdel-Salam
Sugarcane bacilliform virus (SCBV), Badnavirus, Caulimoviridae was detected in the noble sugarcane (Saccharum officinarum) plants in Giza and Assiut governorates showing leaf symptoms of pronounced flecks or freckles and vein banding which turn later to chloro…
16 Aug 2021 - Articles
One economical extraction method to detect all graft transmissible pathogens of citrus
Elharony, S.B, SaharA.Youssef, Richard F. Lee
A simple and applicable extraction method for the isolation of high quality nucleic acid (DNA and RNA) to detect all graft transmissible pathogens of citrus including prokaryotic pathogens, DNA and RNA viruses and viroid provides a new approach to virus and vi…
16 Aug 2021 - Articles
ISOLATION OF AN ENTOMOPATHOGENIC VIRUS IN THE GREEN STINK BUG NEZARA VIRIDULA (LINNAEUS) IN EGYPT
AdlyAbd-Alla, MahaNada, Francois Cousserans, Max Bergoin
The green stink bug Nezaraviridula is an important economic pest in Egypt and in many countries around the world and requires control management measures. During the establishment of a N. viridulacolony in Egypt, a significant reduction in the insect productiv…
16 Aug 2021 - Articles
Partial characterization of Necrotic ringspot virus on apple in EGYPT
Aly M. Abdel-Salam, Samah A. Mokbel
A severe isolate of Necrotic ringspot virus (NRSV) was isolated from apple orchards in the vicinity of Nubaria city, Beheira governorate, Egypt. Infected-apple trees showed chlorotic, necrotic ringspots, and shoot holes on leaves. Severely infectedtrees wither…
16 Aug 2021 - Articles
Expression of Recombinant gD2 Protein in Transgenic Tomato plants for development of a plant-derived vaccine against Human herpes virus 2
Aya El-Turkey, A. K. El-Attar, A. E. Aboulata, B. Othman, K. A. El-dougdoug
Human herpes virus 2 (HSV) is a DNA-viral genome and cause infection for both human and animal. HSV-1 and HSV-2 are causing infection to human. HSV-2 is a sexually-transmitted virus. HSV-2 is prevalence in European countries and also in Egyptian-closed societi…
16 Aug 2021 - Articles
Production of specific antisera against Beet mosaic virus and Beet necrotic yellow vein virus
Allam A. Megahed, Hoda M.A. Waziri, Khaled A. El-Dougdoug, Badawi A. Othman, Sirag M. Lashin, Mohamed D. Hassanin et al.
The ultraviolet spectrum of purified Beet mosaic virus (BtMV) particles obtained by density gradient centrifugation revealed that, the concentration of virus preparation estimated using spectrophotometric measurements at 260 nm was 3.004 mg/ml. The min, max nm…
16 Aug 2021 - Articles
Eradication of potato viruses and evaluation of different soil mixtures on minitubers production
Samah A. Mokbel, Ashraf A. Abd El-Mohsen
Recently production of virus-free potato minitubers is critical. For this purpose, infected tubers with potato leafroll virus (PLRV) or potato virus x (PVX) from two cultivars Spunta or Lady Rosette as demonstrated by serological detection were used as a sourc…
16 Aug 2021 - Articles
Polyclonal antibodies production against Broad bean mottle virus affecting faba bean (Vicia faba L.) plants in Egypt
Eman A. H. Kattab, A. H. Ebrahem, Om-Hashem M. El-Banna, Hanan F. EL-Kammar³
An isolate of BBMV was obtained from naturally infected faba bean plants growing in the experimental fields of Giza Agricultural Research Station showing mottle and distortion of leaves. The identity of the virus was confirmed biologically, serologically and b…
16 Aug 2021 - Articles
Biological and Molecular Identification of Severe Zucchini Yellow Mosaic Virus from Cucurbita pepo cv. Eskandarani
Amel, S.M. Abo-Senna, M.A. Nasr-Eldin, B.A. Othman, A.A. Megahed
Zucchini yellow mosaic virus (ZYMV) was isolated from Cucurbita pepo cv. Eskandarani cultivated in the open field showing severe systemic symptoms. The isolated virus has wide host range belonging to 4 families resulting systemic and local symptoms. Stability…
16 Aug 2021 - Articles
Characterization of Strawberry latent ringspot virus (SLRSV) on strawberry in Egypt
Amira A. Mazyad, A. A. Kheder, Ahmed K. El-Attar, W. Amer, Mahasen, H. Ismail et al.
Strawberry latent ringspot virus (SLRSV) was isolated from symptomless strawberry plants and identified with a specific antiserum (Loewe Biochemica GmbH) using Double Antibody Sandwich ELISA (DAS-ELISA). Virus survey was carried out during 2013 - 2014 in diffe…
16 Aug 2021 - Articles
Squash leaf curl virus (SLCV) incidence and severity on Phaseolus Vulgars in Egypt
Amro A. Farrag, Ahmed K. El-Attar, Om-Hashem M. El-Banna, Ibrahim A. I, H. M. Mazyad
Squash leaf curl virus (SLCV) was isolated from naturally infected common bean plants grown in Egypt. Symptomatic leaf samples were collected from bean fields cultivated in different governorates and tested by PCR using Geminivirus degenerate primers and Squas…
16 Aug 2021 - Articles
Rapid quantitative detection of Enteric viruses in River Nile and drainage water, Egypt
Mohamed I. Azzam, Safaa M. Ezzat, K. A. El-Dougdoug, Badawi A. Othman
Contamination of surface water with enteric viruses is a major public health concern that warrants the need to develop reliable indicators for enteric viruses contamination. This work assesses the bio-diversity of coliphages to use as bio-indicators for viral…
16 Aug 2021 - Articles
Occurrence, Etiology and Molecular Characterization of Phytoplasma Diseases on Solanum lycopersicum Crop in Egypt
Eman A. Ahmed, Osama Y. Shalaby, Emad F. Dwidar, Samah A. Mokbel, Ahmed K. El-Attar
Surveys were carried over the course of the 2012 and 2013 in different areas of tomato-growing fields in Egypt (Giza, Ismailia, Bani-Sweif, Fayoum and Qena) in order to determine the occurrence and distribution of phytoplasma associated with diseased tomato pl…
16 Aug 2021 - Articles
Biological and Serological Studies on Pea Mosaic Virus (PMV) on Cowpea Forage Crop (Vignaunguiculata L. Walp)
M. A. Kararah, Om-Hashem M. El-Banna, Salwa N. Zein, Abd-Elrehiem, A.F
In the present work, Pea mosaic (PMV) was first reported on cowpea (Vignaunguiculata (L.) Walp).PMV was isolated from naturally infected cowpea plants, showing mosaic and choloratic ring spot symptoms, which had been grown in the experimental fields of Giza Ag…
16 Aug 2021 
Research ArticleImproving the Phytosanitary of Fig Plants Infected with Fig Latent Virus by Nanochitosan and Biomagic
Hanaa H.A. Gomaa, Dalia Y.A. Amin, Mona A. Ismail, Basma Hamdy, Khaled A. El-Dougdoug
Fig viruses have been found as naturally mixing infection in many countries in the world and Egypt. The present study aimed to evaluate the potential effect of nanochitosan and biomagic for treatment of the pathological effects related to virus infection in fi…
17 Dec 2023DOI 10.17582/journal.jvs/2023/11.2.9.24
Research ArticleVirus Like Particles: Nanoparticles for Targeted Drug Delivery
Khaled A. El-Dougdoug, Wael S. El-Araby, Rehab, A. Dawoud
Virus-like particles (VLPs) are biological entities at the nanoscale level that are made of viral proteins organized in a morphology that resembles the original virion but lacks the viral genetic material. VLPs are appealing as a system because their proteins…
22 Oct 2024DOI 10.17582/journal.jvs/2023/11.2.25.44
Research ArticleMolecular Characterization of Recent Field Isolate of Lumpy Skin Disease Virus Compared to the Vaccinal Strains
Akram A. Salama, Moustafa A. Zaghloul, Ahmed A. Zaghawa, Mohamed A. Nayel, Ahmed M. Elsafey, Mohamed F. Azooz et al.
The lumpy skin disease virus (LSDV), causes lumpy skin disease (LSD), is a transboundary viral disease of cattle that has a significantglobal economic impact. This study aims to identify the lumpy skin disease virus that circulate…
17 Dec 2023DOI 10.17582/journal.jvs/2023/11.2.1.8- Articles
Antiviral Diversity of Compounds Derived From Silybum marianum
Hanaa H. A. Gomaa, Entsar A. A. Nassar, K. A. El-Dougdoug
Six genotypes of purple and white head flower varieties and one parent type of milk thistle (Silybum marianum L.) grow in the Egyptian desert. These are important plants used for treatment of various liver diseases. All the studied genetic parameters showed va…
16 Aug 2021 - Articles
In vitro osmotic potential for PVY elimination and tuberization of potato by tissue culture technique
Rehab A. Dawood, Entsar A. Nassar, K.A. El-Dougdoug
Potential osmotic of MS medium is a new method for Potato virus Y (PVY) elimination using tissue culture technique. Osmotherapy refers to culture meristem – tip and stem nodes at MS medium saturated with sucrose. Stem nodes of potato were successfully st…
16 Aug 2021 - Articles
Antiviral activity of lactoferrin against Potato virus x in vitro and in vivo
Samah A. Mokbel, Soad H. Taha, Mahmoud M. Abd-El Hamid, Ali H. Hamed
The effectiveness of lactoferrin against Potato virus x (PVX) in vitro and in vivo have been evaluated. Four concentrations of lactoferrin 100, 250, 500 and 1000 mg/L were examined either in vitro culture medium or in vivo (greenhouse). The presence of the vir…
16 Aug 2021