Bone Marrow-Derived Mesenchymal Stem Cell-Conditioned Medium Supplementation Improves In Vitro Maturation and Subsequent Embryonic Development of Buffalo Oocytes
Bone Marrow-Derived Mesenchymal Stem Cell-Conditioned Medium Supplementation Improves In Vitro Maturation and Subsequent Embryonic Development of Buffalo Oocytes
Jun Zhang, Meizhen Ou, Yingqi Li, Junming Sun* and Yiqiang Ouyang*
Fig. 1.
Isolation, culture and identification of buffalo BMSCs: (A) Buffalo fetuses ranging from 10 cm to 18 mm in body length used to isolate buffalo BMSCs. (B) The morphology of primary buffalo BMSCs on day 3, 4 and 5. (C) The morphology of buffalo BMSCs from passage 1 to passage 3. (D) Alkaline phosphatase live staining of buffalo BMSCs; Osteogenic, chondrogenic, and adipogenic induction differentiation of buffalo BMSCs. Scale bars = 100 μm.
