Effect of Different Thawing Methods on Frozen Semen Characteristics and DNA Damage of Indonesian Simmental Bull
Effect of Different Thawing Methods on Frozen Semen Characteristics and DNA Damage of Indonesian Simmental Bull
Syalsa Bella Fitriana1, Noni Ashri Maghfiroh1, Atikah Nur Baity1, Dio Fico Felsidan Diatmono1, Kurniawan Dwi Prihantoko2, Sigit Bintara2 and Diah Tri Widayati2*
Viability of Simmental bull spermatozoa assessed by eosin-nigrosin staining (10x40 magnification); a white-colored head characterizes live sperm (a) and a reddish-purple-colored head characterizes dead sperm (b).
Abnormalities of spermatozoa were assessed using eosin-nigroin (10x40 magnification); head detached and tail detached (a) and sperm with normal morphology (b).
Sperm DNA fragmentation assessed using Halomax® kit (10x40 magnification). sperm with a halo-shape in the head area indicates intact sperm DNA (a) and sperm without a halo-shape in the head area indicates sperm DNA damage (b).
