Articles
183 open-access articles · page 3 of 10
Research ArticleSex determination through Barr bodies of neutrophils in Humans and Dogs
MUSHTAQ HUSSAIN LASHARI, RABIA HAMEED, UMER FAROOQ, MUSADIQ IDRIS, ZIA UR REHMAN
Blood samples of healthy adult humans (n = 40) and dogs (n = 30) grouped as males and females, were collected aseptically through appropriate measures. Thin blood smears were stained using Field stain ‘A’ and ‘B’. The PMNs were counted…
27 Feb 2024- Research Article
Variations in Hepatorenal profile in Hypothyroid subjects of Lahore, Pakistan
ZAHRA NASEEM, MUHAMMAD AMIR IQBAL, SHAAF AHMAD, NABILA ROOHI
Effects of hypothyroidism on liver and renal parameters and effectiveness of thyroid treatment (thyroxine) on these factors in hypothyroid subjects were assessed in the present investigation. A total of 140 subjects (30 subclinical and 50 overt hypothyroid) be…
27 Feb 2024 - Research Article
Identification and cloning of Cry1AB gene from Bacillus Thuringiensis
AMBASH RIAZ, SHAHID RAZA, HIRA MUBEEN
Bacillus thuringiensis (Bt) is a bacterium that can be used as substitute for various chemical compounds especially insecticides. The insecticidal property of B. thuringiensis comes from the production of Crystal protein. The present study is focused on the cl…
27 Feb 2024 - Research Article
Histopathological effects of Lead Nitrate on body tissues of Herring gull (Larus argentatus)
NOREEN RAZA, TASNEEM A. SAQIB, SYED NAEEMUL HASSAN NAQVI, MUHAMMAD ZAHEER KHAN, MAZHAR HIJAZI
The effects of Lead nitrate on liver, gizzard, and kidney of Herring gull (Larus argentatus) were studied. Significant changes were observed after high and low concentrations of Lead nitrate were injected to Larus argentatus. Paraffin sections showed major alt…
27 Feb 2024 - Research Article
Relationship of lung function with obesity in healthy subjects
SAIMA SHARIF, SHAGUFTA NAZ, FARKHANDA MANZOOR, AYESHA NOOR, ZAHRA JAMIL
Obesity exerts negative effects on all the system of the human body, including the respiratory system and therefore has high mortality and morbidity rates. This cross-sectional study was designed to find the relationship of lung function with obesity in health…
27 Feb 2024 - Research Article
Isolation and screening of keratinase producing bacteria from soil
MARIAM AHMED, YESRA ARSHAD, HAMID MUKHTAR
Keratinase (E.C 3.4.99.11) is an extracellular protease having efficient ability to degrade keratin found in feathers and hair. It has various applications including leather, textile, pharmaceutical, detergent and animal feed industries. In current study, kera…
27 Feb 2024 - Research Article
Isolation and morphological characterization of novel bacterial Endophytes from Citrus and evaluation for antifungal potential against Alternaria solani
SEHRISH MUSHTAQ, MUHAMMAD SHAFIQ, MUHAMMAD ASHFAQ*1 FAIZA KHAN, SOHAIB AFZAAL, UMMAD HUSSAIN, MUBASSHIR HUSSAIN et al.
Endophytes have always been a topic of interest for researchers due to their wide variety of benefits to their hosts and their diversity of geographical distribution. In this study, bacterial endophytes were isolated from the leaf midrib of different varieties…
27 Feb 2024 - Research Article
Optimization of extraction process for the recovery of antioxidants from Morus nigra leaves
UMER YOUNAS, SHAHID IQBAL, JAMSHED AKBAR
In current work, effects of different drying procedures as well as of different solvents on extraction of antioxidants from M. nigra leaves have been studied. Initially, samples were dried using different drying procedures (shade, oven and microwave drying) an…
27 Feb 2024 - Research Article
Frequency of Hepatitis C virus genotypes circulating in Gujrat Punjab, Pakistan
MUHAMMAD NAEEM RAZA, KALSOOM SUGHRA, NADIA ZEESHAN
Hepatitis C virus (HCV) infection is primary cause of liver disease that leads toward liver cirrhosis and hepatocellular carcinoma. This study designed to diagnose the prevalence of HCV genotypes circulating in Gujrat, Punjab. Blood samples of 3540 suspected p…
27 Feb 2024 - Research Article
Alterations, complexity and efficient strategies suitable for genetic studies
AFIRA WAQAR, ADNAN RAUF, BUSHRA CHAUDHRY, IKRAM-UL HAQ
Structural design of genetic studies has progressed over the last decade into the most powerful tool for probing the complex human diseases. There are many different approaches for the identification of genetic risk factors of the complex diseases. New pharmac…
27 Feb 2024 - Research Article
Evaluation of Antioxidant and Antidiabetic Activity of Phyllanthus emblica (Fruit)
ASIFA BASHIR, AAMIR MUSHTAQ, TOOBA MEHBOOB
The objective of this study was to explore the scientific basis of traditional usage of Phyllanthus emblica as an anti-diabetic agent in hyperglycemic patients. Ethanolic extract of its dried fruits was analyzed both qualitatively and quantitatively for evalua…
27 Feb 2024 - Research Article
Raw vegetables as a risk factor for parasitic infections in District Mardan, Pakistan
ASMA WAHEED QURESHI, SHUMAILA ALAM
Assessment of parasitic contamination of raw vegetables in District Mardan, Khyber Pakhtunkhwa was carried out by detection of parasitic eggs, cysts and larvae. Seven vegetables including cabbage (Brassica oleracea), lettuce (Lactuca sativa), carrot (Daucus ca…
27 Feb 2024 - Research Article
Sex determination through Barr Bodies of Neutrophils in cattle and buffalo
MUSHTAQ HUSSAIN LASHARI, UMER FAROOQ, AMMARA ARSHAD, MUHAMMAD NAEEM TAHIR, MUSADIQ IDRIS, ANAM KHURSHID et al.
The present study was designed to assess the presence/absence and frequency of Barr Bodies on Polymorph Nuclear Neutrophils (PMNs) as a confirmatory guide towards sex determination in cattle and buffalo. Blood samples of adult cattle (n=26) and buffaloes (n=26…
27 Feb 2024 - Research Article
A Checklist of family Salticidae (Arachnida: Araneae) from some localities of Pakistan
NUSRAT MAJEED, ABIDA BUTT
Salticid fauna of Pakistan had not been comprehensively explored. This study was an attempt to provide inventory of salticids, which would be the baseline documentation for future studies in Pakistan. This survey was conducted from June 2013 to May 2017, to co…
27 Feb 2024 - Research Article
Histopathological and Micrometric studies of Diazinon exposure on Thyroid and Parathyroid Tissues in Mice
SYEDA NADIA AHMAD, MEHWISH NASIR, AQSA NAUREEN, SHAGUFTA ANDLEEB, KAUSAR RAEES, TAHIR ABBAS et al.
Effects of Diazinon (DZ) were studied at 0, 2.5, 5, 10 and 20 mgkg-1 in adult male mice (10 in each dose group) for thyroid and parathyroid histopathology. Tissues were recovered following 48 h of DZ treatment. Micrometric data in terms of follicular size of t…
27 Feb 2024 - Research Article
Seasonal variations of invertebrates diversity & environmental health in intertidal area at Tak Bay, (Kalatoo) Hingol National Park Balochistan Pakistan
ATEEQUE RAHMAN KHOOHARO, PIRZADA JAMALUDDIN A. SIDDIQUI
This study was initiated to discover the distribution & diversity of invertebrate communities on a rocky intertidal shore at Tak Bay, Ormara along the Balochistan coast of Pakistan. Quadrat techniques were used to establish abundance of species. Overall, 4…
27 Feb 2024 - Research Article
Yield performance of Bulgarian race of Silkworm fed on local Mulberry supplemented with Vitamins and Amino Acids
MAIMOONA KANWAL, MUBASHAR HUSSAIN, ZAHEER ABBAS, SHAKIL AHMAD KHAN, FATIMA ZAFAR, RIZWANKHURSHEED
Silkworm rearing is being practiced for commercial purpose in different parts of Pakistan by rural masses as a cottage industry. Silk seed improvement through importation is one of the key initiative towards popularising and ameliorating silk production i…
27 Feb 2024 - Research Article
Evaluation of biocontrol strategies and its synergistic interaction permitting the chickpea plant to trigger the appropriate defense responses against Sclerotium rolfsii
AMNA SHOAIB, AROOJ SHEZAD, ARSHAD JAVAID, SUNDUS AKHTAR, ZOIA ARSHAD AWAN
22 Brassicaceae member i.e. Rhaphinus sativus L. and three species of Trichoderma (T. harzianum, T. viride and T. hamatum) were evaluated for their antifungal effect against Sclerotium rolfsii, the causal agent of collar rot disease in chickpea. In vitro, meth…
27 Feb 2024 - Research Article
Effect of Alloxan on IL-6 Gene Expression in Mus musulus
SYED SHAHID IMRAN BUKHARI, IQRA AZAM, MUDDASIR HASSAN ABBASI, MEERAB DAUD, NADEEM SHEIKH, ANDLEEB BATOOL et al.
Alloxan monohydrate is an organic compound with heterocyclic skeleton; it has been the routine part of all bakery products such as pizza, biscuits and cakes etc. Swiss albino mice of six weeks were divided into control group and experimental group. Experimenta…
27 Feb 2024 - Research Article
Simulation of a Nanoneedle for Drug Delivery by Using MATLAB Fuzzy Logic
MUHAMMAD JAVAID AFZAL, FARAH JAVAID, MUHAMMAD WASEEM ASHRAF, SHAHZADI TAYYABA, MAMOONA ASHIQ, AYESHA AKHTAR
The emerging technology of nanoneedle employ the nanoneedle for the penetration in cell membrane to investigate and manipulate biomedical procedures in the living cells. This study presents a simulated methodology on the basis of fuzzy logic for flow rate of d…
27 Feb 2024