Articles
308 open-access articles · page 13 of 16
ArticlesEvolution of nonstructural (NS1) gene of Egyptian high pathogenic H5N1 Avian influenza viruses
Amany Adel, Abdelsatar Arafa, Hussein A. Hussein, Ahmed El-Sanousi
In the present study, 24 H5N1 viruses were selected on the basis of the phylogenetic analysis of the HA gene sequences to study the genetic evolution of the NS1 gene of such viruses. The viruses were isolated from chickens and ducks in both live bird markets a…
16 Aug 2021- Articles
Expression of Recombinant gD2 Protein in Transgenic Tomato plants for development of a plant-derived vaccine against Human herpes virus 2
Aya El-Turkey, A. K. El-Attar, A. E. Aboulata, B. Othman, K. A. El-dougdoug
Human herpes virus 2 (HSV) is a DNA-viral genome and cause infection for both human and animal. HSV-1 and HSV-2 are causing infection to human. HSV-2 is a sexually-transmitted virus. HSV-2 is prevalence in European countries and also in Egyptian-closed societi…
16 Aug 2021 - Articles
Production of specific antisera against Beet mosaic virus and Beet necrotic yellow vein virus
Allam A. Megahed, Hoda M.A. Waziri, Khaled A. El-Dougdoug, Badawi A. Othman, Sirag M. Lashin, Mohamed D. Hassanin et al.
The ultraviolet spectrum of purified Beet mosaic virus (BtMV) particles obtained by density gradient centrifugation revealed that, the concentration of virus preparation estimated using spectrophotometric measurements at 260 nm was 3.004 mg/ml. The min, max nm…
16 Aug 2021 - Articles
Eradication of potato viruses and evaluation of different soil mixtures on minitubers production
Samah A. Mokbel, Ashraf A. Abd El-Mohsen
Recently production of virus-free potato minitubers is critical. For this purpose, infected tubers with potato leafroll virus (PLRV) or potato virus x (PVX) from two cultivars Spunta or Lady Rosette as demonstrated by serological detection were used as a sourc…
16 Aug 2021 - Articles
Polyclonal antibodies production against Broad bean mottle virus affecting faba bean (Vicia faba L.) plants in Egypt
Eman A. H. Kattab, A. H. Ebrahem, Om-Hashem M. El-Banna, Hanan F. EL-Kammar³
An isolate of BBMV was obtained from naturally infected faba bean plants growing in the experimental fields of Giza Agricultural Research Station showing mottle and distortion of leaves. The identity of the virus was confirmed biologically, serologically and b…
16 Aug 2021 - Articles
Biological and Molecular Identification of Severe Zucchini Yellow Mosaic Virus from Cucurbita pepo cv. Eskandarani
Amel, S.M. Abo-Senna, M.A. Nasr-Eldin, B.A. Othman, A.A. Megahed
Zucchini yellow mosaic virus (ZYMV) was isolated from Cucurbita pepo cv. Eskandarani cultivated in the open field showing severe systemic symptoms. The isolated virus has wide host range belonging to 4 families resulting systemic and local symptoms. Stability…
16 Aug 2021 - Articles
Characterization of Strawberry latent ringspot virus (SLRSV) on strawberry in Egypt
Amira A. Mazyad, A. A. Kheder, Ahmed K. El-Attar, W. Amer, Mahasen, H. Ismail et al.
Strawberry latent ringspot virus (SLRSV) was isolated from symptomless strawberry plants and identified with a specific antiserum (Loewe Biochemica GmbH) using Double Antibody Sandwich ELISA (DAS-ELISA). Virus survey was carried out during 2013 - 2014 in diffe…
16 Aug 2021 - Articles
SYMPTOMATOLOGY AND SEROLOGICALL SURVEY OF VIRUSES INFECTING SUGAR BEET CULTIVATION IN EGYPT
Megahed, A.A, Kh. A.El-Dougdoug, B.A. Othman
A survey was conducted in the main Egyptian sugar beet growing areas to determine the occurrence and incidence of Cucumber mosaic cucumovirus (CMV), Beet mosaic potyvirus (BtMV), Beet necrotic yellow vein benyvirus (BNYVV), Beet curly top geminivirus (BCTV) an…
16 Aug 2021 - Articles
Serological evidence for the presence of infectious avian hepatitis E virus among chicken flocks in Egypt
Serageldeen Sultan, Nabila Osman
This report on the prevalence of avian hepatitis E virus (avian HEV) among chicken flocks in Egypt. A total of 510 serum samples were collected from broiler breeder (n=191), layer breeder (n=235) and commercial broiler chickens (n=84) from different farm flock…
16 Aug 2021 - Articles
Inhibitory effect of cyanobacteria byproducts on Bean yellow mosaic virus on faba bean plant
Radwa M. Shafie, Soha S. M. Mostafa
The inhibitory effect of culture filtrates and fresh biomass of three cyanobacteria isolates i.e., Nostoc muscorum, Spirulina platensis and Oscillatoria sp. was studied, individually or in a mixture, against Bean yellow mosaic virus (BYMV) infection on Vicia f…
16 Aug 2021 - Articles
Abundance and Variation of Virulence of Rhizobiophages Isolated from the Rhizosphere of Some Legume Plants in Egypt
Ramy A. Qabel, Tarek F. El-Arabi, Adel A. Shoukry, Hassan H. Elsebaay, Abdel-Aziz F. El-Hamahmy
The efficiency of Rhizobium bacteria to form a successful symbiosis with legume plants is governed by many factors. The foremost factor that could affect the rhizobia is bacteriophage infection. In this context, this study aimed to investigate the abundance of…
16 Aug 2021 - Articles
Anatomical and Ultrastructural Changes in Tomato and Grapevine Leaf Tissues Infected with Tomato ringspot virus
Om-Hashem M. El-Banna, Maisa A.E. Awad, M.S. Abbas, Hoda M. A. Waziri, Huda S. A. Darwish
Tomato ringspot virus (ToRSV) is one of the most serious viruses affecting grapevine, tomato and other hosts. The virus was isolated from infected grapevine which was characterized by ring spots, mottling and shortening of internodes. And from tomato exhibitin…
16 Aug 2021 - Articles
Ultrastructural changes in tomato plant induced by phytoplasma infection and attempts for its elimination using tissue culture techniques
Eman A. Ahmed, Osama Y. shalaby, Emad F. Dwidar, Samah A. Mokbel, Ahmed K. El-Attar
Light microscopy was used to compare the anatomical characteristics of stem, flower petiole and leaves of infected tomato with phytoplasma with healthy ones in order to reveal anatomical modifications caused by the infection process. The results obtained showe…
16 Aug 2021 - Articles
Molecular evaluation of Peste des petits ruminants (PPR) vaccine
Manar F. Seioudy, Magda M. Sayed, Ahmed A. El-Sanousi, M. A. Shalaby
Peste des petits ruminants (PPR) is a highly contagious, economically important viral disease of sheep and goats with high morbidity and mortality rates. A live attenuated PPR vaccine has been produced in Veterinary Serum and Vaccines Research Institute (VSVRI…
16 Aug 2021 - Articles
Effect of vaccination with Chicken anemia virus vaccines on immune response to inactivated H5 vaccine
Hanan M. El-Zahed, Hanaa A. Mostafa, Nermeen M. El Sayed, Lamiaa M. Omar
In this study two groups of nine week old Hubbard chickens were vaccinated with two different commercial live attenuated Chicken anemia virus (CAV) vaccines. One was vaccinated with 26 P4 strain vaccine subcutaneously and the other was vaccinated with Cux stra…
16 Aug 2021 - Articles
Impact of Potato virus Y on anatomical leaflets and tubers of potato plants
Badr, A. B, Abou-zeid, A. A2, Al-Naggar, A. M1
A pot experiments were conducted on potato (Solanum tuberosum L.cv. cara) plants during the winter season at 2011 to investigation the abnormalities changes of potato leaflets and tubers infected with Potato virus Y. Anatomical features of terminal leaflets i.…
16 Aug 2021 - Articles
Biological, serological and partial molecular characterization of Prunus necrotic ringspot virus isolated from rose in Taif region, KSA
Sahar A. Youssef, Manal A. El-Shazly, Azza G.Farag, Eman A. Khattab
An isolate of Prunus necrotic ringspot virus (PNRSV) was obtained from naturally infected rose plants collected from different rose farms in Taif, KSA exhibiting a wide range of foliar disease symptoms including necrotic spots, wavy lines, oak leaf pattern, le…
16 Aug 2021 - Articles
Characterization of cucurbit yellow stunting disorder virus and development of polyclonal antibodies using recombinant coat protein
A. A. Rezk, K. A. Alhudaib, A. M. Soliman
Cucurbit yellow stunting disorder virus (CYSDV) was isolated from cucurbit plants growing in Eastern Province area in Saudi Arabia and characterized using reverse transcription-polymerase chain reaction (RT-PCR). The nucleotide sequence for the coat protein (C…
16 Aug 2021 - Articles
Protective Efficacy of Combined Vaccines against Newcastle Disease and Infectious Bronchitis Viruses in Broiler Chickens in Egypt
Amir M. Walaa, Ausama Yousif, Walid H. Kilany, Magdy El-Sayed, Ahmed A. El-Sanousi
A study has been carried out to evaluate different local and imported combined inactivated NDV+IBV vaccines and investigate its ability to protect experimental broiler chickens against challenge with wild ND and IB viruses. Two hundred and eighty commercial on…
16 Aug 2021 - Articles
Isolation of Field Isolate of Equine herpes virus-1 Based on Molecular Detection and Characterization of Glycoprotein D
Abd El-Hamid, M.I, Seham, A. ElZeedy, El-Sanousi A.A, Reda et al.
Equine herpesvirus (EHV)-1 is a pathogen of horses, well known for its ability to induce abortion and nervous system disorders neonatal foal pneumonitis, highly contagious among susceptible horses, with viral Transmission to cohort animals occurring by i…
16 Aug 2021