Articles

Impact of Polyethylene Glycol on Newly Established Ovarian Cell Line (Sl-Omi) from Leaf Worm of Spodoptera Littoral's and on Viral Infection

Khamiss l, Omaima, El Helaly, Alexandra, Abol Ela, Said

New cell line from the Egyptian cotton leaf worm ( Spodoptera littoralis ) originated from the insect ovaries tissues was established, cloned and maintained as contentious cell line. Nine serial dilutions of Polyethylene glycol (PEG) were prepared from mother…

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Molecular Characterization and Efficacy of Spodoptera Exigua Nuclear Pol Yiiedros'is Virus

El Sayed l, Yasmein A, Ghareeb, El-Dougdoug, K. A, Bekheetl et al.

The beet army worm Spodoptera exigua (Hubner.) is affected by nuclear polyhedrosis virus, the disease specific polyhedral virus particles was found to be associated with the beet army worm (BAW). Inoculation of SeNPV into S.exigua induced characteristic sympto…

Cytopathic and Histological Effects of Infected Phthorimaea Operculella Zeller (Potato Tuber Moth) with Granulosis Virus (POGV)

Mohamedl, Shimaa D, Ghareeb, El-Dougdoug, K. A, Bekheitl et al.

The potato tuber moth Phthorimaea operculella is a potato major pest in Egypt. Larvae of P.operculella were inoculated with a granulosis virus (PoGP) showed different cytopathic effects . PoGV induced characteristic symptoms of slow motion, white milky appeara…

The Limiting of Climatic Factors and Predicting of Suitable Habitat for Citrus Gummy Bark Disease Occurrence Using GIS

Gummy bark disease is a disorder of sweet orange on sour orange rootstock in Egypt. There is an importance for hot-growing temperatures to symptom development of citrus viroids. The geographical distribution of the gummy bark disease in some world countries de…

Molecular Characterization and Bioinformatics Analysis of Viroid Isolate Associated with Citrus Gummy Bark Disease in Egypt

Sofyl, A.R, Soliman, A.M, Mousal, A.A et al.

This work reports the characterization of Hop stunt viroid which has been isolated previously in Egypt from sweet orange infected with gummy bark disease namely Citrus viroid Il. The native structure of mature, circular forms of the gummy bark agent was detect…

Incidence of Fruit Trees Viroid Diseases by Tissue Print Hybridization in Egypt

El-Dougdougi, Kh. A, Dawoud, Rehab A, Rezk, A.A et al.

Direct tissue printing on membranes has been applied on a large scale for an initial detection of CEVd, HSVd and PLMVd in fruit trees in Egypt. CEVd was detected mainly in sweet orange trees and occasionally in grapevine and mango. 'Ihe principal characteristi…

Genetic Stability in Tissue Culture-Derived Banana Virus Tested using Thiouracil

Aboud, K. A, Gomaa, Hanaa H. A, El—Taholowy, Mohage et al.

Banana bunchy top virus (BBTV) is a destructive viral disease in many countries including Egypt. It causes severe economic losses because infected banana plants produce no fruit. -The tissue culture approach was used to permit the recovery of BBTV-free plantle…

Characterization and Elimination of A TMV Isolate Infecting Chrysanthemum Plants in Egypt

Nassarl, Entsar A, El-Dougdoug, Kh. A, Osmanl, M.E et al.

An Egyptian isolate of a Tobamovirus was isolated and identified from Chrysanthemum cultivation in Egypt. Both biological, serological and sequence analysis of the coat protein gene demonstrated that the virus represents an isolate of the Tobamoviridae Family.…

Detection of xanthomonas vesicatoria Phages in Infected Tomato Plants

Eisa, Nawal A, Abd El-Ghafar, N. Y. Abd EL-Mageed, M.H, Mohamed et al.

Different Phages parasitizing Xanthomonas vesicatoria (the causal agent of the bacterial spot disease of tomato) were isolated from infected leaves of tomato and from tomato rhizosphere soil, using enrichment technique. The phages produced plaques (4-5mm, diam…

Application of Epitopes Prediction for Antibodies Production against Potato Leaf Roll Virus

Bakr, Youssef M, Nour El-Din, Hanan A, Abd EL-Wahed, Waiel F et al.

Epitopes prediction plays a vital role in the development of antibodies production and immunodiagnostic tests. This paper is focused on building models for predicting linear B-cell epitopes based on support vector machine (SVM) and subsequence string kemel. Th…

The Effect of Some Electrolytes on the Susceptibility of BHK to FMD Virus

Mansour, Abeer E.M, Khodeir, M.H, Hussein, A.M.H.•

Three batches of foot and mouth disease virus (FMDV) type O suspension were prepared on 24 hours previously seeded baby hamster kidney (BHK21) cell culture. Each batch was composed of 15 roller cell culture vessels where the first batch represents the blank vi…

Lumpy Skin Disease Virus Identification in Different Tissues of Naturally Infected Cattle and Chorioallantoic Membrane (CAMS) of Emberyonated Chicken Eggs Using Immunofluorescence, Immunoperoxidase Techniques and Polymerase Chain Reaction

El-Kenawy, A. A, El-Tholoth, M. S

Lumpy skin disease virus (LSDV) was detected in 23sampIes collected from clinically diseased and slaughtered cattle showed clinical signs believed to be LSD. These samples include 7 skin lesions and 16 internal organs (lymph nodes (6), lung (4), kidney (3) and…

Enhancing Effect of Ginseng Stem-Leaf Saponins (GSLS) on the Immune Responses in Vaccinated Calves with Fmd Bivalent Vaccine

Rizk, Sonia A, El-garf, Eman M, Talaat, Abeer A

A comprehensive sero-immunological studies were conduced to reveal the adjuvant's effect of Ginseng Stem-leaf Saponins (GSLS) on the immune response of gel adjuvanted Bivalent Foot and Mouth Disease (FMD) vaccinated calves. This study conducted in two calve gr…

Curcuma Longa for Protecting Chicks against Newcastle Disease Virus Infection and Immunosuppressive Effect of Marek's Disease Viral Vaccine

Madbouly, H.M, Saif, M.A, Hussein, A.S

A total of 300 one day old Hubbard chicks were divided into 6 groups (GI-G6: 50 chicks/each) The Gl(control neither vaccinated nor treated), G2 vaccinated with NDV, G3 (vaccinated with MDV Rispen strain), G4 (vaccinated with MDV and NDV) , Group 5 vaccinated w…

Inheritance of Resistance to Bean Yellow Mosaic Virus in Faba Bean Plants

El-Bramawyl, M . A. S. A, El-Beshehy, E. K. F.'

Bean yellow mosaic virus (BYMV) is one of a devastating pathogen that reduces faba bean crop production in Ismailia. Resistance is limited and resistance-breaking isolates are becoming problematic. Effective BYMV disease control strategies will come from a bet…

Biological Control of Cucumber Mosaic Virus by Certain Local Streptomyces Isolates

El-Dougdoug, Kh.A, Ghalyl, M.F, Tahal, M.A

An antiviral producing• Streptomyces species were isolated from soil rhizosphere in Zagazig province of Egypt. In order to identi$r the Streptomyces strains, morphological, physiological, biochemical and antagonism testes were performed. The Egyptian isol…

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Partial Characterization of an Isolate of Cucumber Mosaic Virus from Ismailia Governorate

Elbeshehy, E. K.F, Sallaml, A.A.A

Cucumber mosaic virus (CMV) was isolated from naturally infected cucumber plants Cucumis sativus L. grown in various garden and greenhouses of Ismailia Governorate, Egypt exhibiting systemic mosaic, blistering, fruit malformation and stunted plant growth and i…

Comparison of the Efficacy of Some Regimes of Vaccination for the Control of VVIBDV in Broilers

Abd El-Razakl, A.G, AboElkhair

Two vaccination programs for infectious viral disease (IBD) were compared in broiler chicken with maternal immunity, placed in tow farms, A and B. IBD immune complex vaccine was administered by subcutaneous route, at the hatchery into chicks of farm A at age o…