John G. Bruno1*, Chien-Chung Chao2,3, Zhiwen Zhang3, Wei-Mei Ching2,3, Taylor Phillips1, Allison Edge1, Jeffery C. Sivils1
...reg; UltraRed (AUR; a resazurin-like substrate) version of a rapid (< 1 h) aptamer magnetic bead sandwich assay. The optimal sandwich aptamer combination utilized the same aptamer sequence (designated Rt-18R) for both capture and reporter functions while producing a signal to noise ratio of > 4.0 for detection of ~ 1,000, R. typhi cells. Titration experiments conducted in buffer revealed a limit of detection between 100 and 1,000 R. typhi c...
Muhammad Shoaib1,3*, Arif Ismiyev2,Khudaverdi Ganbarov3, Aygun Israyilova3 and Sajid Umar4
...rial species by using resazurin microplate assay. All the tested compounds exhibited variable antimicrobial properties against various test cultures. All the compounds showed stronger antimicrobial activity against Gram-negative bacteria as compared to Gram-positive bacteria and fungi. Compound III was found to be the most effective compound. These results demonstrate the potential antimicrobial properties of mono and spiro cyclohexane derivatives and a...
ASMA ZAFAR*, MONIBA KHAWAR, MAHNOOR CHAUDHRY, SAYYEDA SANA BATOOL, AMJAD HUSSAIN** & MUSHTAQ AHMAD SALEEM
...mazing bacterial protein azurin can work as an anti-cancerous agent which only target cancerous cells and do not cause any harm to healthy cells and thus minimize the risk of adverse side effects. The interaction of azurin with P53 (tumor suppressor protein), Eph/ephrin receptor and Cadherin enhances the activity of azurin by inducing apoptosis in cancerous cells, disrupting signaling amon...
