Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
... and 1.5 mM. Cooling and equilibration of the diluted semen was done at 4°C for 4 h subsequently filled in straws at the same temperature and were cooled in liquid nitrogen vapors for 10 min. followed by storage at -196°C for assessment. Progressive motility and viability of spermatozoa were significantly higher in 1.5 mM and 1.0 mM L-cysteine supplemented groups in comparison with 0.5 mM or 0 mM. The number of supra-vital hypo-osmotic swelling test po...
Amit Sharma1* and Pankaj Sood2
...tant used, cooling rate, equilibration time, freezing and thawing rate. All these factors should be given due credit for achieving better post-thaw post thaw quality of semen to be utilized in different advanced reproductive techniques. ...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
...niMedica, Poland). After equilibration, the straws were frozen in liquid nitrogen vapour using a computer controlled automatic freezer. After one day, straws were thawed in a water bath at 38ºC for 20 sec and then were examined. Semen analysis included viability, DNA defragmentation and motility of spermatozoa were being estimated. The addition of 2μg / mL of the GnRH analogue significantly (P <0.05) improved the percentage of live spermatozo...
Adnan Khan1, Rifat Ullah Khan1, Muhammad Subhan Qureshi2, Muhammad Mobashar3, Ali Gohar4, Shakoor Ahmad1, Kamran Khan5, Hamayun Khan1 and Shabana Naz6*
...d Nili Ravi) to find out equilibration time (T1, 2 h; T2, 6 h and T3, 6 h) effects on different semen quality parameters. Highest sperm progressive motility, sperm plasma membrane integrity were observed in T2. It reflects high damages at T3 and T1 due to cryo shock and oxidative stress respectively. Sperm DNA integrity and pH were increased linearly from T1 to T3. The dead sperm percentage was significantly (P<0.01) low in groups with semen sample treated ...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...nt at dilution, cooling, equilibration, freezing and thawing stages. They damage functional and morphological characteristics of sperms. Beside these exogenous stresses there is an oxidative stress which damages the spermatozoa endogenously. These stresses can be controlled with the inclusion of antioxidants in semen extender at the time of cryopreservation. In the current study, semen from four (n=4) healthy Nili Ravi buffalo bulls was collected through artif...
Nuzhat Kamal1, Muhammed Sajjad Ansari2*, Bushra Allah Rakha1, Shamim Akhter1, Saima Qadeer1 and Ali Akhter1
...or 4 h at 4°C. After equilibration, cooled semen was filled in 0.5 ml French straw at 4°C and kept over liquid nitrogen vapors for 10 min. Then straws were plunged into liquid nitrogen (-196°C) for storage. After 24 h of storage, straws were thawed at 37°C for 30 seconds and assessed for sperm motility (%), plasma membrane integrity (%), viability (%), normal apical ridge (%) and chromatin integrity (%). Sperm motility was recorded higher in ex...
Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1
... the effect of different equilibration time and post thawing cryopreservation in gaga chicken semen. The research material was 10 male Gaga chickens who were 10 months old, healthy, and normal reproductive organs. Semen was collected using massage methods. This study uses a complete random design of factorial patterns with factor I being the equilibration time and factor II being the different thawing conditions. Fresh...
Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2
...of sperm motility during equilibration and post-thawing compared to P0 and P1. MYE 2mL supplementation in tris diluent has almost the same capabilities as Andromed ® commercial diluent. The ability to maintain cell membrane integrity is indicated by the RR value reaching 77.99%. In conclusion, Supplementation of myrmecodia extract in 2 mL of egg yolk Tris diluent affected sperm protection during the equilibration and cry...
Makruf Arif1, Asmarani Kusumawati2*, Kurniawan Dwi Prihantoko3, Agustina Dwi Wijayanti4, Sri Gustari2
...valuated after dilution, equilibration, and thawing. The evaluation criteria included sperm motility, recovery rate, viability, membrane integrity, and DNA fragmentation. In the post-dilution and post-equilibration phases, there was no significant effect on motility, viability, and membrane integrity (P > 0.05). However, supplementing genistein and glutathione improved the post-thawing results significantly (P < 0.05)....
Siew Ing Nguang, Nur Syafiqah Binti Zainal, Ahmad Mukhlis Bin Hafas, Hou Chew Ha, Connie Fay Komilus, and Asmad Kari*
...and were subjected to an equilibration phase before being frozen and stored in liquid nitrogen. The straws were thawed after 48 hours to evaluate the effect of extender components. After cryopreservation, T1 showed significantly highest viability with 63.4±3.0%. The improved viability in T2 likely resulted from the antibiotics while the reduced viability in T3 may be due to their absence, with values of 45.7±1.9% and 36.6±1.8%, respectivel...
