Souvik Ghosh, Nobumichi Kobayashi
British Journal of Virology
...ionized the study of RVA genomics, providing a plethora of conclusive and vital data on the overall genetic diversity, true origin and complex evolutionary patterns of human RVA strains. In this review, we have summarized and discussed the significance of recent research outcomes obtained from whole genomic analysis of common and unusual human RVAs. ...
Claudia Kohl*, Andreas Nitsche, Andreas Kurth
Email: [email protected]
...iology and medicine. Metagenomics is one of these emerging NGS applications and offers the opportunity to study i.e. whole ecosystems. Basically, the metagenomics approach is similar to well-known shotgun-sequencing, though on a much bigger scale. For instance, the metagenome of a lake would include all the fish, ducks, plants, fungi, bacteria and everything else that belongs to the lake. If we apply this approach to clinica...
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
...TA and GSTT. Comparative genomics and syntenic analysis provided new evidences for better understanding on gene fates post whole genome duplication (WGD) of common carp. The expression patterns of all GST genes were established in various tissues, including brain, heart, spleen, kidney, intestine, gill, liver, skin, blood and muscle of common carp. Expression profiles provided us more evidences to understand GST gene functions as well as their functional evolu...
Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla3
...ally, to assess the phylogenomics, the N gene was amplified and sequenced from sheep and goats (n=12). Nucleotide identity among Hail strains was identified to be 96.2-100%, while identity with previously sequenced Saudi Arabian strains and with reference PPRV retrieved from GenBank was found to be 94.1-98% and 81.2-100%, respectively. Phylogenetic analysis revealed that Hail PPRV strains clustered in four separate groups within lineage IV indicating genetic d...
Tanveer Hussain, Masroor Ellahi Babar*, Akhtar Ali and Fiaz Hussain
... Pakistani camel’s genomics. In efforts to explore the genetics of the camel, we chose to study the Inteferon beta 1 (IFNβ1) gene in in Mareecha camel and non-descript camel breeds in Pakistan. We amplified the IFNβ1 gene through PCR, followed by sequencing. All sequences were aligned and edited through Codon Code aligner and finally a 364 bp portion was selected for further analysis. Dual peaks/heterozygous conditions were identified at positi...
Hadeer M. Mossa, Ausama A. Yousif, Emad A. Aboelsoud, Mahmoud El Gamal, Ahmed El-
Sanousi
...rimers respectively. Metagenomics is done to
evaluate a virus harvest for standardization by applying a suite of genomic technologies and
bioinformatics tools to directly represent the genetic content of entire communities of organisms in the
given harvest.
Results: In this study a 3rd generation sequencing (TGS) was applied using a Nanopore technology
(MinION) based on identification of DNA bases by ...
Ahmed A. ElWakil1, Ausama A. Yousif2 , Adel A. Fayed3, Ibrahim M. elsabagh2, Mahmoud
El Gamal2, Omnia H. Refaei3
... polymerase mRNA and metagenomics analysis using MinIon technology.
Methods: A total of 34 adult female ticks were collected from three LSD clinically disease cattle that
were previously vaccinated using the Egyptian sheep pox (SPPV) vaccine, apparently healthy cattle in
contact with the diseased cattle, and one healthy animal located in a previously infected zone. LSDVspecific
PCR was used to detect LSDV in samp...
Ali Wahdan1*, Mahmoud Ezzat1, Fatma Youssef2, Manal Munier2, Elsayyad M. Ahmed3, Marwa Abo Hashem1
... Resistant factors, Phylogenomics. ...
Muhammad Saif-ur Rehman1, Faiz-ul Hassan1* and Muhammad Sajjad Khan2
...arranted by using latest genomics tools to get insights into the evolution and domestication of indigenous cattle breeds....
Alif Rahman Rohim Puarada*, Safika, Agustin Indrawati
...of paradise, Shotgun metagenomics
...
Rawa Abdulkareem Abd
...dent approaches like metagenomics, single-cell genomic, and metatranscriptomics which have changed the way we study microbes that are never before grown and isolated outside. Biogeochemical cycling basing on either nitrogen or carbon are put under attention here so that the effects of novel microbes on carbon fixation, organic matter decomposition, nitrogen fixation, nitrification, denitrification and ammonification are expressed. Novel microbes’ capacit...
Azhar Ali1, Faheem Ahmed Khan2, Wu Di3, Huang Chunjie3, Muhammad Rizwan Yousaf1, Bilal Ahmed1, Farwa Shakeel1, Nuruliarizki Shinta Pandupuspitasari1, Windu Negara2, Bambang Waluyo Hadi Eko Prasetiyono1*
...ork employed shotgun metagenomics to examine antibiotic resistance genes (ARGs) present in the feces of dairy cattle. The presence of resistance genes in the feces indicates that certain microorganisms carry several resistance genes, which are often found in close proximity and originate from various species. Our findings indicates that the gastrointestinal system of dairy cows harbors a repertoire of 216 antimicrobial resistance genes, which are presumed to c...
Yasser Asaad Hameed Al-Shareef, Firas Hussain Kadim Abawi*
...c analysis sand compared genomics of NDV isolates to publish sequences. Isolates characterized here showed 90.37% sequence similarity to the Iranian isolate Asil/IR/AAA158/2019 (MN370894.1) within velogenic clusters. Taken together, clinical, genetics and molecular biological approaches identified velogenic strains of NDV in poultry which breach the immunity induced by the currently applied vaccines, warranting future monitoring to better device control strate...
Safaa M. Ali1*; Nadia A. Soliman2
Novel Research in Microbiology Journal (2019), 3(6): 590-597
...lase enzyme by using metagenomics. The plan of work composed of collection of different soil samples, isolation of total DNA, fragmentation, cloning, and expression of the isolated gene(s) in the suitable host microorganism. The total genomic DNA was extracted using a kit (QIAGEN), and then digested by different restriction enzymes BamHI. The digested fragments ranging from ~300-5000 bp were ligated, cloned into pUC19 vector, and then transformed into Escheric...
Noha Mostafa Mahmoud*
...microbial ecosystem. Metagenomics has emphasized the diversity of
the gut microbiome; however, it does not provide reliable insight on the dark matter of
microorganisms or the minor populations. As a result of the rebirth of cultural techniques in
microbiology; the field of "culturomics" is created to cultivate the unidentified bacteria that
reside inside the human gut. In the 21st century's discipline ...
Shrikant Verma1; Mohammad Abbas1,2*; Sushma Verma1; Aliya Abbas Rizvi1; Almas Khan1; Syed Tasleem Raza3; Farzana Mahdi1
Novel Research in Microbiology Journal (2021), 5(4): 1313-1324
...of enormous information, genomics and other "omics" like metabolomics; proteomics and transcriptomics; could soon cause the fantasy of personalized medication to turn into a reality. The most recent advancements in precision medication permit the adjustment of helpful approaches in various pathologies based on the particular molecular interpretation of the patient. Precision medication (PM) represents a new way of thinking about infection detection; ...
Ena Gupta1; Neha Mishra2; Pragya Mishra3; Priyanka Singh3*; Uroosa Noor1; Shashi Soni1
Novel Research in Microbiology Journal (2022), 6(4): 1670-1681
...ritical to elucidate the genomics, and genetic pathways linked to susceptibility and transmission of SARS-Cov-2 infection. The current study aimed to shed some light on SARS-Cov-2 infection and COVID- 19 introduction, as well as the evolutionary history, structure, pathophysiology, genetic modulation, diagnosis, and treatment, in order to provide insight into pandemic flinch, its emergence, and progression around the world. The current study also provides a su...
Tran Thi Hong1; Ngo Van Anh1; Nguyen Thi Ngoc Anh2; Nguyen Van Phuong3*
Novel Research in Microbiology Journal (2024), 8(6): 2694-2711
...general....
