Eman M. Farghaly1, Ahmed Samy1,2*, Heba Roshdy
... E.coli, S.aureus and P. haemolytica isolates recovered from mixed infection cases showed (57.1-100%) resistance to highly important antibiotic group (Doxycycline, Tetracycline, Trimethoprim sulfa methoxazole and Chloramphenicol) and showed dissimilar pattern of resistance to critical important antibiotic group. Salmonella isolates showed antibiotic resistance to Nalidixic acid (100%) and Nitrofurantoin (42.9%). Strict biosecurity measures are required to redu...
Iqra Muzammil1, Muhammad Ijaz Saleem1, Amjad Islam Aqib2,*, Ambreen Ashar3, Syed Ashar Mahfooz1, Sajjad ur Rahman4, Muhammad Shoaib4, Muhammad Aamir Naseer1, Imran Khan Sohrani1, Javeed Ahmad1, Razaullah Saqi1, Fizzah Laeeq Lodhi1 and
Qaisar Tanveer5
...2, CI=6.87-31.76), 46.9% haemolytic S. aureus (15/32, CI=30.87-63.56) and 34.4 % biofilm producing S. aureus (11/32, CI=20.41-51.69). Earthen floor type (OR=1.75, p=0.0996), poor drainage system (OR=7.33, p=0.002), pond as source of drinking water (OR=2.05, p=0.179), stall feeding (OR=7.27, p<0.001), 4-6 years of age of goat (OR=4.2, p=0.0874), and teat injury (OR=13.74, p<0.001) were potential risk...
M. Hanafy1, Rehab Elhelw2, Soliman M. Soliman3, Sherif Marouf2*
...as 40.4%, and Mannheimia haemolytica was 7.1%. Using KMT1 gene for identification of the isolates for P. multocida and SSE gene for identification of the isolates for M. haemolytica, the results revealed that six isolates showing positive PCR for Pasteurella multocida and were subject to further phylogenic characterization. M. haemolytica could not be detected by PCR. Identification and ch...
Mirtneh Akalu1,2*, Takele Abayneh2, Esayas Gelaye2, Behailu Tefera2, Teferi Degefa2, Vemulapati Bhadra Murthy1
...les (OMVs) of Mannheimia haemolytica present a wide range of surface antigens. This study was designed to evaluate the immunogenic potential of M. haemolytica serotype A:1 OMVs (MH-OMVs). Fifteen calves were divided randomly into three groups of five calves and immunized with single-dose and booster-dose of 0.15 mg/ml vesicle preparation. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assay of the parti...
Arsalan Maqbool1,2, Faez Firdaus Abdullah Jesse1,3*, Eric Lim Teik Chung3,5, Abd Wahid Haron3, Mohd Azmi Mohd Lila5, Bura Thlama Paul3,4, Khaliq ur Rehman Bhutto3,6
...infected with Mannheimia haemolytica serotype A2 during rainy and hot seasons. Twenty-four healthy female, non-pregnant does were used, and divided into two equal groups, 12 does were further allocated into 3 groups (n=4), control, non-vaccinated and vaccinated in each season. After acclimatization and synchronization procedure, the vaccinated group was administered with 2 mL of alum-precipitated pasteurellosis vaccine while non-vaccinated and control groups w...
Fathin Faahimaah Abdul Hamid1, Mohd Farhan Hanif Reduan1*, Jasni Sabri1 , Faez Firdaus Jesse Abdullah2,Mohammed Naji Odhah1, Nur Athirah Binti Abdul Manaf1, Mohd Jefri Norsidin2 , Siti Nor Che Yahya1, Intan Noor Aina Kamaruzaman1, Nur Zul Izzati Mohd Rajdi1
...nia caused by Mannheimia haemolytica. Evaluation of the hematological and serum biochemistry changes is critical to determine the effectiveness of the treatment approach in reducing the severity of infection. Healthy goats (n=20) were equally divided into 5 groups: Mannheimia haemolytica 107 concentration was inoculated intranasally to all goats except goats of Group 1 which served as the negative control, Group 2 was the po...
Balquees Kanwal1*, Syeda Saba Shah1, Syed Waqas Hassan2 and Farzana Shaheen3
...ch can lead to diarrhea, haemolytic uremic syndrome (HUS), hemorrhagic colitis (HC) and other lethal complications. Consumption of STEC contaminated food has been associated with food related illnesses outbreak. This study aims to evaluate the prevalence of O157:H7 strain of STEC by detection of Stx-1 and Stx-2 genes in 160 chicken meat samples, which are randomly collected from different regions of Northern Punjab, Pakistan. Isolation of pathogen from meat sa...
Md. Rashedul Islam1, Abul Farah Md. Hasanuzzaman1*, Md. Latiful Islam2, Ghausiatur Reza Banu1
...hery. V. mimicus, V. parahaemolyticus, V. harveyi and V. alginolyticus were the major species identified from the Vibrio genus. The average concentrations of Vibrio spp. in brood, brood’s tank water, eggs, larvae, larval tank water, and larval feed were log10 3.86±0.61 cfu g-1, log10 3.63±0.57 cfu mL-1, log10 2.73±0.13 cfu g-1, log10 2.86±0.21 cfu g-1, log10 2.93±0.25 cfu mL-1 and log10 3.23±0.37 cfu mL-1 respect...
Fahad A. Al-Abbasi1*, Abdullah Samer Habib1, Vikas Kumar2 and Firoz Anwar1
...occus aureus, Vibrio parahaemolyticus and Pseudomonas. Five healthy workers directly involved with processing, handling from various departments without any prior disease were selected for blood profiling. Significant increase in S. aureus colonies were observed in fish samples compared to chicken in the month of September and October, 2017 and in fish and chicken meat from December, 2017 to June, 2018. In all samples TPC was more or less same for fish and chi...
SHEHLA AKBAR*, SAIQA ISHTIAQ*, KHALID HUSSAIN, ANS MUNIR & SAIRA REHMAN
Hafiza Mehwish Iqbal1, Salman Khurshid1*, Saqib Arif1, Qurrat-ul-Ain Akbar1, Saba Iqbal2, Shahid Yousaf3, Kainat Qureshi1, Abdul Karim Khan5, Abdul Ahad6, Aqeel Ahmed Siddique4 and Neelofar Hamid7
...ive bacteria (Vibrio parahaemolyticus and Escherichia coli) and fungi viz., Aspergillus niger, Alternaria alternate, Fusarium solani and Aspergillus flavus were studied by Well diffusion method. Three solvent extracts Aqueous, Acetone and Methanol with concentrations (5,10,15 and 20%) was prepared. Our study showed that maximum zone of inhibition (ZOI) was observed against bacteria for M. Koenigii i.e., 22mm and 23 mm for fungi, while; O. basilicum and S. asoc...
Mercy Cuenca-Condoy1*, Lourdes Reinoso-García2, Juan González-Rojas2, Dionel García-Bracho3
...omogenes, Staphylococcus haemolyticus, Staphylococcus xylosus, Staphylococcus epidermidis, Streptococcus uberis, Streptococcus agalactiae, Lactobacillus mali, Moraxella osloensis, Kocuria salsiccia, Bacillus weihenstephanensis, Corynebacterium xerosis, Macrococcus canis, Chryseobacterium bovis, Rothia endophytica, among others. However, Staphylococcus chromogenes, Lactobacillus mali and Staphylococcus aureus were the most frequent bacteria in cases of subclini...
Maysoon S. Abbas, Shaimaa N. Yassein
... samples (13.5%), V. parahaemolyticus for 38 out of 400 samples (9.5%), V. vulnificus for 14 out of 400 samples (3.5%), and V. alginolyticus for 23 out of 400 samples (5.75%). Four virulence factors were studied for nine isolates of V. cholerae. Phospholipase activity showed a positive result in 6 out of 9 isolates (66.66%), proteinase activity in 7 out of 9 isolates (77.77%), while hemolytic activity and biofilm formation were positive in all V. cholerae isol...
Ying Zhong1,3,4, Jingni Chen1, Chunping Huang1, Huaiyuan Jin1, Jinlu Huang1, Lining Zhao1, Yi Geng3, Guiping Wang1,4 and Xueqiao Qian1,2*
...bacteria were Vibrio parahaemolyticus and that they carried the pirA and pirB virulence genes. These bacteria showed β-hemolysis with translucent rings, and six serotypes were identified. The regression infection results showed that the lethal rates of the three V. parahaemolyticus strains of serotypes O1:KUT and OUT:KUT to L. vannamei were significantly higher than those of the other serotypes. The three V. para
Sohaila Fathi El-Hawary1, Nermeen M.L. Malak2, Reda A. Gomaa3, Hesham Z. Tawfeuk3, Suzan Ismail4, Nady Khairy Elbarbary5*
...olerae and V. parahaemolyticus. Samples were 150 fish (30 of each Nile tilapia, Nile perch, sardine, Mugil cephalus, and Sea bass) from fish supermarkets. Sardine samples had the highest Vibrio count (4.77±1.22 log10 CFU/g), followed by and Sea bass (4.62±1.35 log10 CFU/g), Mugil cephalus (3.50±1.74 log10 CFU/g), Nile perch (3.24±1.05 log10 CFU/g), while Nile tilap...
Afnan Ahmed Allhyani1, Mohamed Baeshen1, Nagwa Thabet Elsharawy2,3*
...teritidis and Vibrio parahaemolyticus). Finally, the different concretions of each extract were added to the chevon. Obtained results observed that all extracts have antibacterial effects to varied degrees against all tested microorganisms, and the extracts succeeded in extending the shelf life of chevon to about 17 days. The study results indicated that the natural products had a potent effect against the tested pathogens. So, it can be a natural alternative ...
SaimaMajeed1*,TayyabaAsif2, Aisha Qureshi3,AyeshaUmer3 and Naveed Ahmad3
...rio cholerae, Vibrio parahaemolyticus, Listeria monocytogene, and Staphylococcus aureus werelikewiseinspectedyetdiscoveredmissinginallthesamplesunderexamination. All the frozenseafoodsampleswerejudgedasprotectedfoodfromamicrobiologicalperspectiveandmethods are satisfactorylimitquantifiedbytheintern...
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