Proximate analysis and in vitro biological evaluations of Misopates orontium (L.)
Proximate analysis and in vitro biological evaluations of Misopates orontium (L.)
SHEHLA AKBAR*, SAIQA ISHTIAQ*, KHALID HUSSAIN, ANS MUNIR & SAIRA REHMAN
ABSTRACT
Proximate analysis plays an important role in assessing the
appropriateness of medicinal plants or their extracts orally taken by the
marginal communities. Primary and secondary metabolites of Misopates
orontium (L.) belongs to Plantaginaceae family were screened out and
tested for therapeutic values through in-vitro biological assays.
Quantitative analysis was done on powder of Misopates orontium by
using standard methods for estimating the primary and secondary
metabolites. The maximum value of total carbohydrates (11.04μg/ml),
total starch (14.69μg/ml), total protein (14.34μg/ml), total amino
acids(28.43μg/ml), total lipids (3.67 μg/ml), total glycosaponins (14.95%),
total alkaloids (24.67%), total polyphenolics (28.40%) and total flavonoids
(5.67%) were found in given plant samples. were found in the samples.
Haemolytic and DNA protection studies of Misopates orontium were also
performed. Solar protection factor was calculated of all fractions by
adopting the standard procedure. By using concentration 200μg/ml and
400μg/ml methanolic fraction showed 4.57 and 4.86, hexane 1.54 and
5.0, aqueous 1.07 and 2.33, chloroform 0.94 and 3.87, ethyl acetate
18.56 and 28.41, butanol 4.34 and 7.91. Ethyl acetate fraction showed
highest SPF value. Presence of polyphenols and flavonoids provide an
evidence of this plant being traditionally used in the treatment of various
ailments. The results of present study give an evidence for the existence
of diverse primary and secondary metabolites in Misopates orontium and
thus rationalizes its use in traditional medicines for the cure of different
aliments
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June 2020
Vol.66, Iss. 1, Pages 1-126
