Bacillus subtilis BML5 Isolated from Soil Contaminated with Poultry Waste has Keratinolytic Activity
Aqsa Imtiaz and Abdul Rehman*
...study aim was to isolate keratinolytic bacteria from the soil contaminated with poultry waste. From amongst nine bacterial keratinase producing isolates were found. The BML5 isolate was identified as Bacillus subtilis based on biochemical characterization and 16S rRNA Ribotyping. B. subtilis BML5 showed maximal keratinase activity at 37°C and at pH 8. An increase i...
Nay Naing Htoo1, Basit Zeshan1,2,*, Aung Tun Khaing1, Than Kyaw1, Erkihun Aklilu Woldegiorgis1 and Mohd Azam Khan1
...pillary surface area and keratinized layer of CFR (4.15mm2, 50.54µm), were not significantly different from CF (3.81mm2, 49.26µm) (P>0.05) but it was significantly different from kids in DM group (1.17mm2, 26.36µm) (P<0.05). The rumen papillary surface area and keratinized layer were also significantly different between CF and DM (P<0.05). The rumen of kids from CFR...
Yang Liu1,2,3, Baimei Liu1,2,3, Chenchen Li1,2,3 and Meiwen An1,2,3,*
...e collagen metabolism in keratinocytes and fibroblasts co-culture under pressure, and study the role of IL-1α and MMP-3 in terms of regulating fibroblasts-keratinocytes collagen metabolism with pressure.Chitosan-gelatin scaffolds were fabricated by vacuum freeze drying. Keratinocytes and fibroblasts were seeding on scaffolds to form systems as: keratin
Ramazan İlgün1*, Nilgün Kuru2, Ferhan Bölükbaş3 and Fatih Mehmet Gür4
...tongue were covered by a keratinised stratified squamous epithelium. Lamina propria and submucosal layers were distinguishable underneath the epitelium. SEM observations of surface of the radix linguae revealed conical papillae and tongue caudally directed lingual papillae. Thus, in this study, the anatomy and histology of the tongue of the guinea fowl tongue were examined in detail using light and scanning electron microscopy, and the similarities and differe...
Larysa Kladnytska1, Anatoliy Mazurkevych1, Natalia Bezdieniezhnykh2, Oleg Melnyc1, Sergiy Velychko3, Mykola Malyuk1, Vasyl Danilov1, Yuriy Kharkevych1 and Magdalena Gryzinska4*
...of expression of pan cytokeratin – 299 ± 0.6, actin – 299 ± 0,58, vimentin – 265,7 ± 20,7, E-cadherin – 298,3 ± 1 points, indicating that this cells have a high adhesive properties, proliferation activity, cell signaling, interaction and mobility. The level of expression of these proteins remains high in the stem cells culture also at the Xth passage, although it is significantly lower in relation ...
Bibi Safia Haq1,2, Hidayat Ullah Khan3, Abdul Shakoor4, Shahnaz Attaullah5, Ishrat Rahim1
PROJECTION ETCHING OF KERATIN THIN FILMS FOR FABRICATION OF SKIN STRUCTURE USING ARF EXCIMER LASER
...nomenology of the etched keratin patterns have been investigated
and the surface modification was analysed. For effective studies of skin treatments, large areas of model skin need
to be generated. Therefore an ArF excimer Laser wavelength (193nm) with the ability to pattern square mm areas
per pulse was used even though the maximum repetition rates are typically an order of magnitude slower. Keratin
and the surface modification was analysed. For effective studies of skin treatments, large areas of model skin need
to be generated. Therefore an ArF excimer Laser wavelength (193nm) with the ability to pattern square mm areas
per pulse was used even though the maximum repetition rates are typically an order of magnitude slower. Keratin
Bibi Safia Haq1,2, Shahnaz Attaullah3, Abdul Shakoor4, Hidayat Ullah Khan5, Khan Alam5, Kausar Shaheen1
CHARACTERISTICS AND EFFICACY OF ULTRAFAST LASER PULSES FOR BIOMEDICAL APPLICATIONS
...rtar structure in
keratin films to investigate skin structures for different cream absorption. The laser characterised here is Spectra
Physics, Hurricane X. This is a complex laser system consisting of a seed laser and a regenerative amplifier. It is
important to have knowledge of these characteristics to be able to predict the transport of the beam through various
optical elements and also to analyse the subsequent interaction with...
keratin films to investigate skin structures for different cream absorption. The laser characterised here is Spectra
Physics, Hurricane X. This is a complex laser system consisting of a seed laser and a regenerative amplifier. It is
important to have knowledge of these characteristics to be able to predict the transport of the beam through various
optical elements and also to analyse the subsequent interaction with...
Muhammad Ajmal1, Saima Mustafa1, Fizza Ibrahim Bajwa1, Cheng Zhou2, Guangdong Wen2, Soe Lwin Myint2, Syed Irfan Raza3, Ihtasham Bukhari4, Mubashir Hassan5, Muhammad Faisal6 and Furhan Iqbal1*
...nt of papular lesions of keratin-filled cysts over extensive areas of the body. Two consanguineous families were enrolled from Basti Mochi Wala, Mouza Gulab Shah in Muzaffargarh District of Punjab (Pakistan) having multiple siblings suffering from alopecia. The aim of this study was to find out the genetic mutation(s) in hairless (HR) gene, if any, in the enrolled subjects. A questionnaire was filled for each subject on the sampling site in order to col...
Imran Tarique1*, Muhammad Ghiasuddin Shah1, Illahi Bux Kalhoro1,
Zaheer Ahmed Nizamani2, Mansoor Tariq 2, Jamil Ahmed Gandahi1,
Saqib Ali Fazlani3 and Benazir Sahito3
...lium, and main cells was keratinocytes. The dermal-epidermal junction was regular with an absence of dermal papillae and epidermal ridges. Dermis layer possess dens irregular connective tissue, which consist of elastic, collagen and reticular fibers. Superficial dermal papillary layer was located beneath the epidermis, whereas dermal reticular layer deeper above the hypodermis. The papillary and reticular der...
Essam H. Ibrahim1,2,3, Attalla F. El-kott1,4, Ali Alshehri1, Mona Kilany2,5, Reza Yavari6, Salahud Din7* and Diaa Massoud8,9
... tongue was covered by a keratinized stratified squamous epithelium. A dense connective tissue composed the lamina propria and thick masses of striated muscles constituted the bulk of the tongue. The lamina propria didn’t penetrate completely into the filiform papillae of the lateral margin. Numerous forms of lingual salivary glands with seromucous secrations were intermingled with the lingual muscles in the lingual root.
...
Ho-Seong Cho1 and Yeonsu Oh2,*
...trongly reactive for cytokeratin and CD10 and were negative for carcinoembryonic antigen, fetoprotein and hepatocyte paraffin-1. Taken together, the tumor was diagnosed as cholangiocarcinoma. This is the first case report of a cholangiocarcinoma in the puma.
...
Jing Fu
... 2 (SATB2 Group) and Cytokeratin 20 (CK20 Group) in mucinous ovarian tumors and their correlation with tumor pathological classification and prognostic outcome. One hundred and sixty cases of ovarian mucinous tumors diagnosed from January 2018 and January 2020 were selected. They were divided into four groups: mucinous cystadenocarcinoma group (age 62.78±7.92, n=53), borderline mucinous cystadenocarcinoma group (age 63.82±9.26, n=55), mucinous cy...
Jian-Cheng Zhai1,2, Sheng-Nan Wang1,3, Qiang-Hui Wang1, Yan-Ling Xia1, Wei-Shi Liu1, Ya-Jie Yin4 and He-Ping Li1*
Farha Deba1, Rubina Nelofer2 and Muhammad Irfan1*
...strains that can produce keratinase were isolated and identified from the abattoir region. A transparent, clear zone was evident in each isolate during the initial primary screening on skimmed milk agar plates. The secondary screening was then evaluated via submerged fermentation utilizing raw feathers as a substrate for keratinase production. On skimmed milk agar plates with streaked pure culture growth patterns, these seve...
Al Salihi karima Akool1* , Almas. M. Al-Bayati2, Iman Mousa Khaleel3
...by a stratified squamous keratinized epithelium. Furstenberg’s Rosettes were vertically located as ridges at teat internal streak canal end mucosa. The number of Furstenberg’s Rosettes ranged from 11 to 15 and 9 to 11 in SCPMG and SCN-PMG, respectively. Mammary gland parenchyma revealed an arrangement of lobules separated by interlobular connective tissue. Each lobule comprised a group of alveoli surrounded by interalveolar connective tissue. In SC...
Muhammad Talha Sajjad1, Hamid Akbar1*, Muhammad Arif Khan1, Muhammad Hassan Mushtaq3, Shehla Gul Bokhari1, Muhammad Abid Hayat4 and Ghulam Mustafa2
... ultrasonography and cytokeratin staining. Wound re-epithelialization and the number of fibroblasts were assessed by cytokeratin staining. All data were statistically analyzed. We observed that PRP-wounds had highly significantly (P<0.01) increased levels of neovascularization on days 14 and 28 than control wounds. PRP-wounds had highly significant (P<0.01) increases in re-epithelialization levels and fibroblast number...
MARIAM AHMED, YESRA ARSHAD & HAMID MUKHTAR*
...cient ability to degrade keratin found in feathers and hair. It has various applications including leather, textile, pharmaceutical, detergent and animal feed industries. In current study, keratinolytic bacteria were isolated from the soil of poultry farm and different cultural conditions were optimized to obtain maximum enzyme yield. The isolates were initially screened by formation of zone of clearance on the skimmed milk ...
Rabab Abd Alameer Naser1*, Sameer Ahmed Abid Al-Redah2, Enas S. Ahmed3, Fatimah S. Zghair2, Ali Ibrahim Ali Al-Ezzy4
...sa was lined by a thick, keratinized stratified squamous epithelium. Immunohistochemical analysis revealed the presence of serotonin receptors in this layer. The density of glands in the lamina propria increased distally. Turkeys possessed tubular acinar glands, while ostriches had simpler tubular glands. The submucosa was less distinct in turkeys compared to ostriches, where it was well-developed. The muscularis layer also differed between the two species.
Saif Dhulfiqar Abdulhasan Al-Khafaji, Siraj Moner Al-Kafagy*
... part of the tongue. The keratinized layer in the body of tongue was thick layer at the cranial part and then absent gradually toward the root of the tongue in both birds. The root in both birds was lined with non-keratinized epithelium. The lingual gland give positive reaction for alcian blue stain in both birds that mean the gland was have acidic mucopolysaccharid, for periodic acid shiff the lingual gland in the tongue of...
Khalid Hadi Kadhim1*, Iman Mousa Khaleel2, Firas Abbas Hussen3
...m corneum was covered by keratinized stratified squamous epithelium in all regions of skin (neck and tail) except in abdomen was non-keratinized. The dermis of the abdomen skin of males was thicker than in females, while the dermal thickness of females in both neck and tail skin was higher than in males. The results of hypodermis showed high loose connective tissues and adipose tissues. Whereas, the tail region was contained...
Eze, E.M.1*; Ezebialu, C.U.2; Unegbu, V.N.3; Nneji, I.R.4
Novel Research in Microbiology Journal (2019), 3(3): 379-386
...isolate and identify the keratinophilic fungi and other dermatophytes present in soils of Otolo Nnewi, Nnewi north local government area, Anambra state, Nigeria. Eighty soil samples were collected from four habitats (playgrounds, abattoir, public parks, and poultry farms) of Otolo Nnewi; and were screened for the presence of keratinophilic fungi and dermatophytes, using hair baiting techniques for isolation of these fungi. O...
Wen-Da Wang1, Wei-Yue Gong1, Bin Li2, Shan-Shan Lei3* and Qing-Hua Wang1*
...lammatory responses, and keratinization, while down-regulated DEmRNAs were enriched for intermediate filament organization and tight junctions. RT-qPCR analysis showed that the expression of lncRNA NEAT1, miR-135b-5p were significantly increased (p <0.05) compared to the normal group, while the levels of lncRNA MALAT1, lncRNA XIST, miR-125b-5p, miR-125b-2-3p, KIF2C, MYLK, ACTG2 and MYH11 were significantly decreased (p <0.01 and 0.05). In conclusion, the...
Pakistan Journal of Zoology
November
Featuring
-
Morphometric Measurements, Gonopod and Gonopores Appearances in Early Sexual Crablet Stage of Scylla paramamosain (Estampador, 1949)
Muhammad Nur Syafaat, Che Zulkifli Che-Ismail, Adnan Amin-Safwan, Mohamad N. Azra, Mohammad Syahnon, Ambok Bolong Abol-Munafi and Mhd Ikhwanuddin
Pakistan J. Zool., Vol. 57, Iss. 2, pp. 759-766
-
Preparation of High Selenized Gastrodia elata Blume Polysaccharide and its Immunomodulatory Effects on RAW264.7 Cells and Cyclophosphamide-Treated Mice
Fengwei Ma, Qihua Wen, Qingfang Deng, Yihao Lu, Buyan Zhang, Yongyou Cheng and Su Xu
Pakistan J. Zool., Vol. 57, Iss. 2, pp. 747-758
-
Freshwater Fish Biodiversity in Liangzi Lake
Ke Li, Hai Liu, Jiangang Zhou, Hongbing Chen and Zhengxiang Wang
Pakistan J. Zool., Vol. 57, Iss. 2, pp. 737-745
-
Histopathological and Molecular Analyses of Leptospira borgpetersenii and Leptospira interrogans in Bovine Kidneys in Kota Bharu, Kelantan, Malaysia
Intan Noor Aina Kamaruzaman, Siew Zee Ong, Iman Natasha Sofea Jafri, Dayangku Umi Aqilah Pengiran-Isa, Mohamad Sabri Abdul-Rahman, Mohd Farhan Hanif Reduan, Thilini Nisansala, Soon Heng Goh, Siti Nur Haslina Mamat, C.W. Salma C.W. Zalati, Sazaly AbuBakar and Shih Keng Loong
Pakistan J. Zool., Vol. 57, Iss. 2, pp. 727-736
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