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Dual Role of Multicopper Oxidase of Klebsiella pneumoniae as a Copper Homeostatic Element and a Novel Alkaline Laccase with Potential Application in Green Chemistry
Soumble Zulfiqar1, Khuram Shehzad1, Sana Tahir1, Khalid A. Al-Ghanim2 and Abdul Rauf Shakoori1,2,3,*
...ve been shown to possess laccase activity. In this study, we have cloned and over-expressed the CueO of locally isolated Klebsiella pneumoniae KW strain. The CueO protein was purified to homogeneity by nickel affinity chromatography. Enzyme assays of CueO protein with phenolic substrates revealed its laccase activity. The kinetic studies showed Km value of 0.2µM, kKcat 0.68 S-1 and Kcat/km 1.2S
Optimization of Cultural Conditions for the Treatment of Pulp and Paper Industrial Effluent by Pleurotus ostreatus (L.)

Azeem Haider1, Muhammad Mohsin Alam2*, Azhar Ali Khan3 and Muhammad Asif Zulfiqar3

...iz; xylanase, CMCase and laccase production by Pleurotus ostreatus were optimized in shake flask cultures. The results indicated that the optimum fermentation medium in shake flask studies contained a carbon (glucose 4%), nitrogen (0.5% ammonium chloride), inoculation level (three disks, 0.5 cm in diameter), temperature (30 °C), incubation period (at 7 days) and initial pH 5.0. Under these culture conditions, the maximum level of xylanase activity (9.29 Um...
An Overview of Production and Industrial Exploitation of Bacterial Laccases
Fehmina Ashraf1*, Muhammad Irfan2, Hafiz Abdullah Shakir1, Shaukat Ali3, Muhammad Khan1
...al and textile etc. Both laccases and mediators have been used in different process like delignification of pulp. Laccases from bacterial species are used in dye decolouration and biobleaching processes. This review article describes the whole overview of laccases.
Emergence of Cryptococcus spp. in Donkeys in Egypt: A Potential Public Health Concern
Rahma Mohamed, Sara Nader, Dalia Hamza and Maha A. Sabry*
...ates demonstrated higher laccase (LAC1) genes among the identified virulence factors. While capsular associated protein (CAP59) gene identified alone or associated with LAC1 gene in the other 2 C. gattii isolates. This study underlines a potential association of those fungi with human disease in Egypt. In order to strengthen existing therapeutic and control approaches, further analyses of the main risk factors and the other virulenc...
Purification and Characterization of Laccase Enzyme from Locally Isolated Aspergillus flavus Strain

Syeda Rubab Zaidi* and Safdar Ali Mirza

...tion and optimization of laccase enzyme production from locally isolated and screened potential fungal strain of Aspergillus flavus was monitored. During this study culture medium and different parameters such as incubation period and inoculum size were optimized and inducers were employed for enhanced production of laccase. Partial purification was accomplished through Ammonium-Sulphate precipitation and for further purific...
Optimization of cultural conditions for enhanced production of laccase by Aspergillus flavus Maf 0139

MADIHA AFTAB1, ARIFA TAHIR*1, TAYYABA ASIM1 & IRFANA MARYAM2

...echnological process for laccase production has gained great attention. Present study is concerned with the enhanced production of laccase by Aspergillus flavus Maf 0139. Five different cultural media were tested and best one was selected for laccase production. Different cultural conditions such as inoculum size, pH, temperature, incubation period, moisture content and inducer were studie...
Optimization of Laccase Production Parameters by Aspergillus niger from Different Eco-Logical Sites of Lahore

Syeda Rubab Zaidi* and Safdar Ali Mirza

...g of fungal isolates for laccase enzyme production from different environmental samples of soil, water and air obtained from selected areas of Lahore. A total 248 strains of microscopic fungi were cultured by using serial dilution method and plate assay method. Pure cultures of these strains were further inoculated on PDA (Potato Dextrose Agar) plates supplemented with indicator compounds guaiacol or tannic acid to check the laccase

Punjab University Journal of Zoology

December

Vol.39, Iss. 2, Pages 135-238

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