Optimization of Cultural Conditions for the Treatment of Pulp and Paper Industrial Effluent by Pleurotus ostreatus (L.)
Optimization of Cultural Conditions for the Treatment of Pulp and Paper Industrial Effluent by Pleurotus ostreatus (L.)
Azeem Haider1, Muhammad Mohsin Alam2*, Azhar Ali Khan3 and Muhammad Asif Zulfiqar3
ABSTRACT
The efficacy of different Pleurotus ostreatus (L.) isolates to treat pulp and paper industrial effluent on a shake flask were studied. In the shake flask studies, Pleurotus ostreatus (L.) decolorized the effluent 69.68 %, on 7th day of incubation. Various cultural conditions including different glucose concentrations, nitrogen sources, pH, temperature and incubation period influence on biomass and different enzymes viz; xylanase, CMCase and laccase production by Pleurotus ostreatus were optimized in shake flask cultures. The results indicated that the optimum fermentation medium in shake flask studies contained a carbon (glucose 4%), nitrogen (0.5% ammonium chloride), inoculation level (three disks, 0.5 cm in diameter), temperature (30 °C), incubation period (at 7 days) and initial pH 5.0. Under these culture conditions, the maximum level of xylanase activity (9.29 Uml-1), CMCase (7.37 Uml-1), laccase (13.57 Uml-1) and effluent decolorization (69.68 %) were observed on 7th day during shake flask studies.
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