A Comparison of Landrace Boars Sperm Quality in Tris-Egg Yolk Dilution with Different Carbohydrate Supplements
A Comparison of Landrace Boars Sperm Quality in Tris-Egg Yolk Dilution with Different Carbohydrate Supplements
Wilmienthe Marlene Mesang Nalley1*, Aloysius Marawali1, Kirenius Uly1, Petrus Kune1, Franky Ms Telupere1, Ni Made Paramita Setyani1, Alvrado Bire Lawa1, Yustiany Yuliana Bette1, Thomas Mata Hine1, Abdullah Baharun2
ABSTRACT
This study aims to improve boar sperm viability during storage by adding various carbohydrates (sucrose, lactose, and fructose) to Tris-egg yolk diluents , providing additional energy substrates that may extend sperm viability and motility. Good-quality sperm samples, with motility levels of ≥70% and abnormalities <20%, were diluted in a TEy control solution, with additional treatments including sucrose (TS), lactose (TL), fructose (TF), and combinations of the carbohydrates (TSF, TLF). The diluted samples were stored at 18-20°C and evaluated at 12-hour intervals until motility decreased to 40%. Statistical analyses were performed using ANOVA and Duncan’s test. The addition of sucrose to the Tris-egg yolk (TS) diluent significantly (P<0.05) enhanced sperm motility (51%) and viability (58%) until the 60th hour. While the TS group showed enhanced longevity, there were no significant differences between TS, TL, TF, and TSF groups (P>0.05). The survival rate of sperm in the TS diluent was notably higher than the control, TL, TF, and TLF groups (P<0.05) but did not significantly differ from the TSF group (P>0.05). The TS diluent also exhibited the lowest rate of sperm abnormalities, significantly differing from all other diluents (P<0.05). Overall, the addition of sucrose to the Tris-egg yolk diluent emerged as the most effective method for maintaining sperm quality in landrace boars, with implications for improving sperm preservation protocols.
Keywords | Landrace boar, Sperm preservation, Sperm motility, Sperm viability, Tris-egg yolk diluent, Carbohydrate supplementation
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