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An Improved Heamagglutination Inhibition Test for Rapid Diagnosis and Stereotyping of Avian Infectious Bronchitis Virus

An Improved Heamagglutination Inhibition Test for Rapid Diagnosis and Stereotyping of Avian Infectious Bronchitis Virus

Johar Hussian1, Faiz Muhammad2, Shafqat Fatimah Rehmani3, Aqeel Ahmad4, Nazir Ahmad Lone4, Moomal Bughio5, Syed Khurram Freed1 and Shakeel Ahmed Khan4*

1Department of Microbiology, University of Karachi, Karachi
2Department of Microbiology, Balochistan University of Information Technology, Engineering and Management Sciences (BUITEMS), Quetta, Balochistan
3Quality Operation Lab, University of Veterinary Sciences, Lahore 
4Department of Biosciences, Barrett Hodgson University, Karachi
5Poultry Research Institute, Karachi
 
* Corresponding author: [email protected]

Fig. 1.

The poly peptide profile of IBV four major glycoprotein and two non-glycoproteins. Lane 1, 2 and 3 IBV sample and low molecular weight marker (bio rad).

Fig. 2.

RT-PCR profile of IBV S1 gene on agarose gel using primer pair New S1 oligo 5/ and degenerate 2/ wit the product size 1700 bp. Lane1, 2, 3 show amplified product of IBV S1 gene and Lane 4 DNA marker fermentas.

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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