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Analysis of Metabolites from Purple Cleome Extract (Cleome rutidosperma Linn.) as Potential Organic Fungicides

Analysis of Metabolites from Purple Cleome Extract (Cleome rutidosperma Linn.) as Potential Organic Fungicides

Ali Ikhwan1*, Dian Indratmi1, Faridlotul Hasanah1, Manar Fayiz Mousa Atoum2,3 and Irum Iqrar4,5

Ali Ikhwan1*, Dian Indratmi1, Faridlotul Hasanah1, Manar Fayiz Mousa Atoum2,3 and Irum Iqrar4,5
 
1Department Agrotechnology, Faculty of Agriculture and Animal Science, University of Muhammadiyah Malang, Jl. Raya Tlogomas No. 246, Malang 65144, Indonesia; 2Molecular Biology and Genetics, The Hashemite University, PO Box 330127, 13133 Zarqa, Jordan; 3Department of Medical Laboratory Sciences, The Hashemite University; 4Department of Biotechnology, Quaid-i-Azam University, NCB Building Islamabad 45320, Pakistan; 5Pakistan Academy of Sciences, 3 Constitution Ave, G-5/2, Islamabad Capital Territory, Pakistan.

*Correspondence | Ali Ikhwan, Agrotechnology Department, Faculty of Agriculture and Animal Science, University of Muhammadiyah Malang, Indonesia; Email: aliikhwan64@gmail.com 

ABSTRACT

One disease that often attacks chili (Capsicum L.) is anthracnose caused by Colletotrichum capsici [(Syd.) E.J. Butler & Bisby]. In the field, C. capsici is controlled with a chemical fungicide that harms the environment. The purple Cleome plant (Spider plant – Cleome rutidosperma Linn.) can be extracted and function as an organic fungicide for environment-friendly control. This research aimed to examine the type and concentration of purple cleome extract metabolites and understand their effectiveness in inhibiting C. capsici. Purple cleome leaf is extracted with 1:1 w/v absolute methanol and concentrated with 1:1 v/v cold absolute methanol, and then the supernatant is purified using a centrifuge. The metabolic analysis was performed using Gas Chromatography-Mass Spectrometry (GC-MS) and comparing the data with the National Center for Biotechnology Information (NCBI) database to obtain specific metabolites. The inhibition test was done with one control (P0: chemical fungicide) and two-level of treatment, i.e., the concentration of purple Cleome extract (P1: 80 % and P2: 40 %), then ANOVA and HSD test were carried out to find out the best treatment. The purple Cleome leaf extract contained three metabolite compounds as organic fungicides, namely propanoic acid 4.12 %,  phenol 8.38 %, and isopropyl myristate 15.86 %. The inhibition test confirmed that 80 % (P2) purple cleome extract could suppress C. capsici attack up to 16.67 %, significantly higher than chemical fungicide (24.4 %).

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Sarhad Journal of Agriculture

September

Vol.40, Iss. 3, Pages 680-1101

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