Anatomical and Ultrastructural Changes in Tomato and Grapevine Leaf Tissues Infected with Tomato ringspot virus
Anatomical and Ultrastructural Changes in Tomato and Grapevine Leaf Tissues Infected with Tomato ringspot virus
Om-Hashem M. El-Banna1, Maisa A.E. Awad2, M.S. Abbas3, Hoda M. A. Waziri2 and Huda S. A. Darwish2
ABSTRACT
Tomato ringspot virus (ToRSV) is one of the most serious viruses affecting grapevine, tomato and other hosts. The virus was isolated from infected grapevine which was characterized by ring spots, mottling and shortening of internodes. And from tomato exhibiting necrotic rings and sinuous lines symptoms. The virus was checked in the suspected samples by ELISA using the specific antiserum. Anatomical and ultrastructure changes in leaf tissues of both Tomato and Grapevine artificially infected with Tomato ringspot virus were studied by light and electron microscopy. By light microscopy viral infection resulted in the presence of amorphous inclusion bodies in the cytoplasm of infected leaves.Phloem tissues were also affected by infection. Investigations of leaf tissues of both tomato and grapevine by Transmission Electron Microscope (TEM) revealed several ultrastructure changes in each of chloroplast, mitochondria, and the nucleus. The ultrastructure changes expanded also to the cell wall, cell membrane and the endoplasmic reticulum.
The chloroplast exhibited different degrees of malformation and lysis. The mitochondria were highly vacuolated and the nucleus was misshapen. On the other hand the cytoplasm contained large crystalline inclusion bodies. These anatomical and ultrastructure changes were not observed in the tissues of healthy plants, indicating the effect of the virus of these tissues.
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July 2014
Vol. 11, Iss. 2, Pages 1-310
