Anthelmintic Effect of Silver Nanoparticles of Citrullus colocynthis on Leishmina tropica Isolated from Pet Animals In Vitro
Anthelmintic Effect of Silver Nanoparticles of Citrullus colocynthis on Leishmina tropica Isolated from Pet Animals In Vitro
Abdulameer Jawad Zayier*
ABSTRACT
The current study aim to prepare several types of extracts (aqueous, hot, cold, alcoholic, and nano) from the colocynth plant to determine the inhibitory effect of these extracts on the number and vitality of the Leishmania tropica promastigotes in vitro ,The results shown that even at low concentrations (0.5 mg), the colocynth plant’s green-produced silver nanoparticles demonstrated strong antileishmanial activity against Leishmania tropica promastigotes during a 24-hour incubation period. The development of the parasites was significantly inhibited both in number and viability of promastigote that treated with green AgNPs of Citrullus colocynthis comparing with control. It also caused killing the promastigote on third day after treatment in all concentration of green nanoparticles of plant (0.5, 1, 1.5 and 2) grams and shown best results in lowering viability in two days after treatment , While the alcoholic extract had an greatest significant inhibitory effect at concentration of 2 grams on the eighth day of incubation, killing of the 100% of the live parasites was observed comparison with the control group No any significant effect of the hot and cold aquatic colocynth extracts was observed on the vitality of promastigotes. Only the tenth day of treatment at concentration of 2 grams had a slight significant decrease in the vitality of the promastigotes.
Keywords | Silver nanoparticles, Citrullus colocynthis, Leshmina tropica, Pet animals
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November 2024
Vol. 12, Sp. Iss. 1
