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SJA_36_4_1214_1226

 

 

 

Research Article

Biochar Improves Phenological and Physiological Attributes of Wheat in Soil Amended with Organic and Inorganic Nitrogen Sources

Mushtaq Ahmad Khan*, Abdul Basir and Beena Saeed

Department of Agriculture, University of Swabi, Anbar Swabi Khyber Pakhtunkhwa, Pakistan.

Abstract | Application of biochar (BC) to soil has the ability to improve soil fertility on sustainable basis under nitrogen (N) and organic matter deficient environments. Therefore, the present study was conducted to enhance N availability from organic and inorganic N sources via biochar (0, 10, 20 and 30 tons ha-1) under four levels of N (0, 90, 120 and 150 kg ha-1) during Fall 2015-16 and 2016-17. The required N was met from urea, poultry manure (PM) and farmyard manure (FYM). Application of 20 and/or 30 t BC ha-1 significantly delayed booting, anthesis, physiological maturity in wheat, and resulted in higher leaf area (LA), leaf area index (LAI), plant height, number of tillers and spikes m-2 when compared to control. Whereas, application of 150 kg N ha-1 as urea, FYM and PM, delayed booting, anthesis, physiological maturity in wheat and increased plant height and spikes m-2 of wheat. Similarly, 120 and 150 kg N ha-1 applied from PM, FYM and urea resulted in higher LA, LAI, tillers and spikes m-2 over control plots. Hence, application of 120 kg N ha-1 either from organic or inorganic sources along with 20 t BC ha-1 shall be applied to wheat as this combination positively enhanced wheat growth and development.


Received | June 16, 2020; Accepted | July 13, 2020; Published | November 29, 2020

*Correspondence | Mushtaq Ahmad Khan, Department of Agriculture, University of Swabi, Anbar Swabi Khyber Pakhtunkhwa, Pakistan; Email: [email protected]

Citation | Khan, M.A., A. Basir and B. Saeed. 2020. Biochar improves phenological and physiological attributes of wheat in soil amended with organic and inorganic nitrogen sources. Sarhad Journal of Agriculture, 36(4): 1214-1226.

DOI | http://dx.doi.org/10.17582/journal.sja/2020/36.4.1214.1226

Keywords | Biochar, Leaf area, Nitrogen, Nitrogen sources and wheat


Introduction

Wheat production needs to be improved either by adopting good management or through change in genetic techniques (Borisjuk et al., 2019). Phenology of a crop play significant role towards yield of the crops (Ihsan et al., 2016). The balance between growth stages like vegetative and reproductive are vital for the whole crop performance and ultimately for yield (Mathan et al., 2016). During vegetative growth a carbon source in the various plant parts (shoot and root) is established which is further utilized during reproductive phase (Holland et al., 2019). Growth, development and the time of assimilates partitioning in plant body is generally determined by phenology (Gonzalez-Navarro et al., 2016). To make sure the proper balance between vegetative and reproductive growth stages of the crops there is an urgent need for nutrients management on sustainable basis (Sawan, 2013).

Nitrogen (N) is the key nutrient among essential plant nutrients which affects crop morphology, phenology, growth and yield (Abid et al., 2016). It is most abundant in environment, but most deficient in soil. Moreover, its deficiency affecting agriculture productivity throughout the world and particularly in Pakistan (Maqsood et al., 2016). The application of N to wheat from synthetic source is influential as it increases wheat tillers m-2 (Khattak et al., 2017), leaf area, leaf area index, plant height, spikes m-2 and delay booting, anthesis and physiological maturity in wheat (Stumpf et al., 2019; Sher et al., 2018).

Since green revolution, the productivity of cereals (especially wheat) had largely increased due to the application/success of inorganic fertilizers (Ali et al., 2012). Though the significance of inorganic fertilizer cannot be overlooked however, the role of inorganic fertilizer has been much lesser in term of improving soil fertility (Hammad et al., 2010). Therefore, there is an increasing trend for the practice of organic manures including FYM and PM in order to get higher yield without disturbing soil health (Ali et al., 2017). To handle this situation and pay off loses due to inorganic N fertilizations, the addition of organic sources of nutrients are considered the best possible solution. Because organic manures hold maximum potential for enhancing soil characteristics in the long run (Timsina, 2018). Organic manures are used to restore exhausted/low fertile soils Hammad et al. (2011), and to get maximum yield without disturbing the soil health (Arif et al., 2013). Due to the improvement in soil organic matter, water storage, cation exchange capacity (CEC), water infiltration, aeration, porosity and biological activity (Agbede, 2019). Jan et al. (2018) stated that among organic manures PM is the richest source of essential nutrients which promote vigorous vegetative growth which further delay wheat phenology. Further, integration of organic and synthetic fertilizers delayed days to 50% heading, enhanced LAI, plant height, yield and its components of wheat (Sadur et al., 2010).

In Pakistan the use efficiency of N fertilizers is almost 30 to 50%. This low efficiency might be due to N loses as ammonia volatilization, nitrous oxide emission as a result of de-nitrification and nitrate leaching (Haroon et al., 2019). Thus biochar (BC) incorporation to soil may relief this problem through nutrients addition or improving soil CEC (Silva et al., 2017). Furthermore, BC as a soil amendment play starring role in holding water in the soil (Jalal et al., 2016). Slowly releases nutrients (Arif et al., 2014). And reduced nutrients leaching (Sara et al., 2018). Thus, greater nutrients concentration in the rhizosphere (Sial et al., 2019). Therefore, enough food available throughout the plant growing periods which ultimately delayed maturity (Arif et al., 2014).

Biochar (BC) had robust attraction for ammonium and nitrate ion (Fidel et al., 2018). Thus, enhanced fertilizers use efficiency by minimizing loses of N and ultimately increased its uptake (Du et al., 2019). Which further have positive impact on plant height (Agegnehu et al., 2015). Moreover, incorporation of 25 t BC ha-1 delayed booting, anthesis and maturity stages in wheat (Ali et al., 2015). Delayed leaf senescence by keeping photosynthesis active (Rizwan et al., 2018). Thus, delayed wheat phenology (Ali et al., 2015), and ultimately increased yield and its components (Uzoma et al., 2011). Similarly, Ali et al. (2015) noted more numbers of spikes for 25 t BC ha-1 amended plots. Furthermore, Batool et al. (2015) observed minimum LA for BC control plots when compared with BC amended plots. Moreover, application of BC at 2% positively affected number of tillers per plant during two consecutive seasons (Baronti et al., 2014).

Keep eyeing on the significance of wheat phenology, its contributions towards yield and its response to various fertilizers sources (organic and synthetic). The current experiment was conducted to observe the influence of BC and different N management system on phenological and physiological attributes of wheat under alkaline soils.

 

Materials and Methods

Description of the study area

The current field experiments were carried out during Fall 2015-16 and 2016-17 at Agriculture Research Station (ARS) Swabi Khyber Pakhtunkhwa Pakistan, located at latitude 34.12o and longitude 72.47o. Mean monthly air temperature and total monthly rainfall were recorded through an automated weather station installed at the experimental site. Total monthly rain fall was in the range of 0 mm during (April, May, Nov. Dec. 2016, March, May, June 2017) to 188 mm (March 2016) while mean monthly air temperature was in the range of 11.89 oC (Dec. 2015) to 34.68 oC (June 2017) during the crop growing season (Figure 1). Rain fall changes during the years. Therefore, to evade the water stress due to shortage of water the plants were frequently irrigated. During 2015-16 the crop was irrigated four times (6 Dec. 28 Dec. 2015 and 25 Jan. 30 Feb. 2016) while during 2016-17 the crop was irrigated six times (5 Nov. 28 Nov. 23 Dec. 2016 and 2 Feb. 27 Feb. 21 March 2017). As a result, crop received optimum supply of water. A surface (0-15cm) composite soil sample was taken from the experimental field before treatments application and was analyzed for base line data (Table 1). The soil was silt loam soil texture, alkaline (7.6) and non-saline (0.64 d Sm-1) in nature having a bulk density of 1.29 g cm-3. Moreover, the soil was low in organic matter (0.41%), having total C (0.24%), P (4.6 mg kg-1), N (0.002 %) and K (66 mg kg-1).

 

 

Table 1: Physio-chemical properties of experimental field.

Property

Unit

Value

pH

----

7.6

EC

d Sm-1

0.64

C

%

0.24

N

%

0.002

P

mg kg-1

4.6

K

mg kg-1

66

Organic matter

(%)

0.41

Texture

----

Silt loam

Bulk density

g cm-3

1. 29

 

Biochar production

The BC used in this experiment was produced using a ‘on farm’ method suitable for small-scale production of BC in Pakistan. Dodonaea viscosa (L.) Jacqa (Local name: Ghwraskay) wood was pyrolysed at 300-500o C for 3-4 hours. Further, it had pH of 7.05, EC 1.49 d Sm-1, C (59.35%), P (0.112%) and N 0.08% (Table 2).

Details description of the experiments

This research study was laid down using 2 factorial randomize complete block (RCB) design with split plot arrangements having 40 treatments each replicated 3 times. The study was consisted of four levels of wood biochar (0, 10, 20, 30 t ha-1) and N management (0, 90, 120, 150 kg ha-1). The required amount of N was applied from urea, FYM and PM. Moreover, FYM and PM were applied at the rate of (10341.67, 13791.67, 17241.67 and 5883.33, 7841.67, 9800 kg ha-1) in order to provide 90, 120 and 150 kg N ha-1. Wood BC was applied to the main plots one month before sowing and was incorporated with the help of rotavator moreover; biochar, FYM and PM were analyzed for its chemical composition (Table 2). Analysis showed that FYM had pH of 8.57, 2.39 dS m-1 EC, 46.4% C, 0.34% P, 0.87% N and 0.6% K while PM having pH of 6.82, 2.48 dS m-1 EC, 34.7% C, 0.97% P, 1.53% N and 0.9% K. Based on the aforesaid chemical analyses the required amount of N was met from FYM and PM and was properly incorporated in sub-plots by hoeing in order to avoid mixing with the adjacent sub plots. The required amount of N from urea was calculated from the percent N present and was applied to sub-plots in split doses (half at sowing and half at tillering stage) while all the FYM and PM were applied one month before sowing. Sowing was done on November 8th 2015 and November 5th, 2016. Wheat variety Pirsabaq 2013 was used as a test crop. Seed rate (120 kg ha-1) was used in 3 x 4 m2 plots by maintaining row to row distance of 30 cm. A basal dose of P and K at 90 and 60 kg ha-1 was calculated from both single super phosphate and potassium sulphate, respectively to all plots. Sowing was done by hand hoe in each subplot. Normal cultural practices were maintained until harvesting of the crop.

 

Table 2: Characteristics of biochar, PM, FYM used in the experiment.

Property

Unit

Biochar

PM

FYM

pH

-----

7.05

6.82

8.57

EC

d Sm-1

1.49

2.48

2.39

C

%

59.35

34.7

46.4

P

%

0.112

0.97

0.34

N

%

0.08

1.53

0.87

K

%

----

0.9

0.6

 

Field observations

Phenological observation like days to boot stage was measured by counting total days between sowing till fifty percent plants attained booting. While counting total days between sowing till fifty percent plants extruded anthers showing clear indication of anthesis and to know about physiological maturity, difference in days was noted between sowing till fifty percent plants physiologically mature. Disappearance of green color from the glumes was considered the best sign to know about the exact time of the said stage (Khan et al., 2017). Agronomic parameter like plant height was measured (at physiological maturity) by averaging height of 5 randomly selected plants in every subplot (Arafat et al., 2016). For measuring leaf area (at anthesis), leaves from the randomly selected five tillers were passed through the equipment (Li-car model no A-3000) while leaf area index was calculated with help of scan canopy analyzer (Delta-T Devices LTD Burwell, Cambridge, UK) (Sher et al., 2018). Data about tillers and spikes m-2 were noted down by counting the total tillers and spikes in an m-2 area of each subplot (Khan et al., 2008).

Characterization of soil, biochar, FYM and PM

Soil bulk density (BD) was calculated by the formula as described by Blake and Hartge (1986). Soil organic matter (SOM) was determined by the method of Nelson and Sommers (1982). Soil pH and EC in 1:5 soil water suspension determined by the procedure of McLean (1982); Rhoades (1996), respectively. Soil carbon was found by the procedure of Nelson and Sommers (1982). Total nitrogen data was measured calorimetrically by Kjeldahl method as described by Bremner and Mulvaney (1982). ABDTPA Extractable potassium was determined by the procedure as recommended by Ryan et al. (2001) through Flamephotometer (Jenway, UK). While the ABDTPA extractable P content in soil samples was determined by the method as described by Soltanpour and Schwab (1977).

Statistical analysis

The obtained data was examined statistically through Satatistix 8 (Version 8.1, Copyright©, 1985-2005) according to two factorial RCBD. In case of significant F test, further analyses of various means were done through least significant difference (LSD) test at (5%) probability value (Steel and Torrie, 1980).

 

Results and Discussion

Days to boot stage

Data on phenological parameters of wheat as affected by BC and N management are presented in Table 3. Booting were significantly delayed with increasing BC level from 0 to 20 t ha-1 but further increasing BC level up to 30 t reduced days to booting. More days to booting (111.2) were counted for those plots where 20 t BC was applied. This was followed by 30 t BC treated plots (110.6). The treatments 20 and 30 t BC did not show any statistical differences when compared to each other however an increase of (2.77 and 2.22%) were observed respectively over control. Minimum days to booting (108.2) were taken by BC control plots, where no BC was applied. Plots treated with PM, took 109.5 to 111 days to booting when compared to those plots which received urea, whose range was 109.2 to 111 days. Similarly, plots received FYM as N source had a range of 109.2 to 109.8 days up to booting. More specifically, application of highest quantity of N (150 kg) applied from urea, PM and FYM significantly increased days to booting (4.23, 4.23 and 3.09%) over control. This was followed by 120 kg N applied from PM and FYM, having an increase of (3.91 and 3.83) over control. Interaction between BC × N showed that combined application of 20 t BC and 120 kg FYM N took more days to booting 113.83 (Figure 2).

 

 

Days to anthesis stage

Anthesis (Table 3) was significantly delayed in plots subjected to 20 t BC. This was followed by 10 t BC amended plots and BC control plots. The treatments 20 and 30 t BC did not show any statistical differences when compared to each other. N control plots took less days to anthesis (131.1) when compared to N treated plots. Generally, plots having highest level of N from urea, FYM and PM delayed anthesis more when compared to lower levels of N applied from the same sources. More specifically, application of 150 kg N from urea, FYM and PM took highest days to anthesis 141.5, 142 and 142.4 when compared to other levels of N and N control plots. Similarly, the BC × N interaction (Figure 3), showed that application of 20 t BC along with 150 kg N from urea took more days to anthesis 147.17.

 

Table 3: Days to booting, days to anthesis, days to physiological maturity, plant height, leaf area, leaf area index, tillers and spikes m-2 of wheat as affected by biochar and nitrogen management.

Biochar (t ha-1)

Days to

Booting

Days to Anthesis

Days to Maturity

Plant height

Leaf Area

Leaf Area Index

Tillers m-2

Spikes m-2

0

108.2 c

136.1 c

165.4 c

96.4 c

82.65 c

2.33 c

280.7 c

263.7 c

10

108.8 b

138.8 b

167.6 b

98.7 b

107.16 ab

3.15 ab

291.8 b

272.7 b

20

111.2 a

140.9 a

168.8 a

100.7 a

111.52 a

3.34 a

297.4 a

281.4 a

30

110.6 a

140.9 a

169.4 a

101.1 a

100.82 b

3.06 b

300.9 a

282.8 a

LSD (0.05)

0.60

1.21

1.11

1.44

7.66

0.24

4.77

5.92

Nitrogen Management (kg ha-1)

Control

106.5 d

131.1 e

161.3 e

89.4 e

76.67 d

1.91 e

249.0 e

223.5 e

90 N Urea

109.2 c

139.5 c

168.2 bc

97.6 d

86.33 cd

2.43 d

281.5 d

264.3 d

120 N Urea

109.2 c

139.5 c

167.8 bc

100.7 bc

103.45 ab

3.07 abc

295.1 bc

278.4 c

150 N Urea

111.0 a

141.5 ab

169.3 ab

103.9 a

104.49 ab

3.21 abc

305.4 a

288.1 a

90 N FYM

109.2 c

137.0 d

165.6 d

96.3 d

99.58 b

2.93 bc

293.3 bc

278.7 bc

120 N FYM

110.6 ab

140.3 bc

169.1 abc

100.1 c

107.12 ab

3.27ab

303.1 a

285.0 abc

150 N FYM

109.8 bc

142.0 ab

170.5 a

103.6 a

107.62 ab

3.31 ab

305.4 a

287.9 ab

90 N PM

109.5 c

138.7 cd

167.5 c

97.5 d

98.32 bc

2.84 c

288.6 cd

268.8 d

120 N PM

110.7 ab

139.8 c

168.1 bc

100.6 bc

109.98 ab

3.38 a

304.6 a

286.3 abc

150 N PM

111.0 a

142.4 a

170.6 a

102.6 ab

111.82 a

3.39 a

300.7 ab

290.6 a

LSD (0.05)

0.95

1.77

1.75

2.27

12.12

0.38

7.55

9.35

2015-16

108.0 b

137.5 b

166.1 b

98.40 b

94.28 b

2.67 b

281.44 b

264.59 b

2016-17

111.4 a

140.8 a

169.5 a

100.05 a

106.79 a

3.26 a

303.91 a

285.71 a

Interactions

Y x BC

0.779

0.860

0.983

0.932

1.00

0.989

1.00

0.977

Y x N

1.000

0.992

1.000

0.995

1.00

1.00

1.00

0.919

BC x N

0.000 **

0.000 **

0.000 **

0.278

0.00**

0.00**

0.00**

0.00**

Y x BC x N

0.900

1.000

1.000

1.000

1.00

1.00

1.00

1.00

 

 

Days to physiological maturity

Both BC and N management had significantly delayed days to physiological maturity in wheat (Table 3). Physiological maturity was delayed by169.4 days in plots treated with 30 t BC when compared 10 t BC ha-1 and BC control plots (167.6 and 165.4 days) but were not significantly varied from plots treated with 20 t BC (168.8 days). Generally, N treated plots took more days to maturity (from 168.2 to 170.6) when compared to N control plots (161.3 days). More specifically, plots received 150 kg of N applied from PM, FYM and urea delayed maturity by (170.6, 170.5 and 169.3 days), followed by 120 kg N applied from FYM (169.1 days). Similarly, BC × N interaction showed that 30 and 20 t BC treated plots took more days to maturity when 120 and 150 kg N were applied from PM and urea. Furthermore, 30 t BC along with 120 and 150 kg N applied from FYM also took more days to maturity (Figure 4).

Plant height

Significant effect of BC and N were observed for plant height of wheat (Table 3). Visually tallest plants were recorded for 30 and 20 t BC incorporated plots (101.1 and 100.7 cm), when compared 10 t BC (98.7 cm) and BC control plots (96.4 cm). The treatments 30 and 20 t BC did not show any significant differences when compared to each other. Application of N resulted in increased plant height when compared to N control plots. Generally, plant height was increased as N levels were increased from lower to higher. For urea it was in the range of (97.6 to 103.9 cm), for FYM (96.3 to 103.6 cm) and for PM the range was (97.5 to 102.6 cm). Moreover, tallest plants (103.9, 103.6 and 102.6 cm) were noted in those plots where 150 kg N was applied from urea, FYM and PM respectively. In case of years maximum plant height (100.05 cm) were observed during 2016-17.

 

 

Leaf area

Both BC and N management and its interaction significantly affected leaf area (LA) of wheat (Table 3). Plots treated with 20 t BC had increased LA up to (37.18 cm2) when compared to 30 t BC (33.60 cm2) and BC control plots (27.54 cm2), but not significantly different from 10 t BC (35.72 cm2). Generally, LA was increased as N levels were increased from lower to higher. For urea it was in the range of (28.77 to 34.83 cm2), for FYM (33.19 to 35.87 cm2) and for PM the range was (32.77 to 37.28 cm2). More specifically, higher LA (37.28 cm2) was obtained in those plots where 150 kg N was applied from PM when compared to 90 kg N applied from urea, FYM and PM (28.77, 33.19 and 32.77 cm2), but significantly not different from 120 kg N applied from urea, FYM and PM. Significantly lower LA was noted by N control plots (25.56 cm2). In case of years maximum LA (35.59 cm2) was observed during 2016-17. In case of BC × N interaction, enhanced LA was measured for 30 t BC along with 120, 150 and 120 kg N applied from FYM, FYM and PM. Furthermore, 20 t BC along with 150, 150, 90 and120 kg N applied from urea, PM and FYM also resulted in maximum LA. Similarly, those plots where 10 t BC and 150 kg N was applied from PM and FYM also gives maximum LA (Figure 5).

 

 

Leaf area index

Both BC and N management and its interaction significantly affected leaf area index (LAI) of wheat (Table 3). Plots amended with 20 t BC had increased LAI up to (3.34) when compared to 30 t BC (3.06) and BC control plots (2.33), but not significantly different from 10 t BC (3.15). Furthermore, LAI was in the range of (2.43 to 3.21) for urea, for FYM (2.93 to 3.31) and for PM it was in the range of (2.84 to 3.39). Significantly greater LAI (3.39 and 3.38) was measured by those plots where 150 and 120 kg N was applied from PM, this was followed by 90 kg N applied from urea, FYM and PM (2.43, 2.93 and 2.84), but significantly not different from those plots where 150 and 120 kg N was applied from urea and FYM. Significantly lower LAI was measured for N control plots (1.91). Mean data of years showed that maximum LAI (3.26) was measured during 2016-17. In case of BC × N interaction, significantly maximum LAI was measured for 30 t BC treated plots when combined with 120, 150 and 120 kg N applied from FYM, FYM and PM. Furthermore, 20 t BC incorporated plots also resulted in maximum LAI when applied with 150, 150, 90 and120 kg N applied from PM, urea, FYM and PM. Similarly, those plots where 10 t BC and 150 kg N was applied from FYM also gives maximum LAI (Figure 6).

 

 

Tillers m-2

BC, N management and its interaction significantly affected tillers m-2 of wheat (Table 3). Those plots where 30 t BC was applied produce maximum tillers (300.9) compared to 10 t BC (291.8) and BC un-treated or control plot (280.7) but were not significantly different from those plots where 20 t BC was applied. Furthermore, tillers were significantly increased by N when compared to N control plots. Specifically, Plots treated with 150 kg N applied from urea produced more tillers (305.4), when compared to 90 kg N applied from urea, FYM and PM (281.5, 293.3 and 288.6), but not significantly different from 120 and 150 kg N applied from FYM and PM (303.1, 305.4, 304.6 and 300.7). In case of years, maximum tillers (303.92) were observed during the 2nd year (2016-17). In case of interaction between BC × N, maximum number of tillers was produced by those plots where 20 t BC and 150 kg N was applied from PM (Figure 7).

 

 

Spikes m-2

BC, N management and its interaction had significant impact on spikes m-2 of wheat (Table 3). Those Plots where 30 t BC was applied produce maximum spikes (282.8) compared to 10 t BC (272.7) and BC control plot (263.7) but were not significantly different from those plots where 20 t BC was applied. Number of spikes was enhanced by N application when compared to N control plots. For N management it was range from (264.3 to 290.6). More specifically, those plots where 150 kg N was applied from PM produced more number of spikes (290.6), when compared to 90 kg N applied from urea, FYM and PM (264.3, 278.7 and 268.8), but were not significantly varied from 150 kg N applied from urea, FYM (288.1 and 287.9) and 120 kg N from PM and FYM (286.3 and 285.0). in case of years, highest number of spikes (285.71) were produced during 2nd year (2016-17). Moreover, interaction between BC × N showed that, maximum spikes (332) were produced by 20 t BC treated plots along with 150 kg N applied from PM (Figure 8).

 

 

Biochar (BC) incorporation significantly delayed different growth stages, occurred during the growth period of wheat. Because as a soil amendment BC played a starring role in improving water holding capacity (WHC) of the soil, slowly release nutrients, reduced nutrients leaching, improves nutrients concentration and nutrients use efficiency (NUE), hence enough food available during the growing period of the plants, which delayed maturity (Mierzwa et al., 2019). Our results of late flowering and maturity were also confirmed by (Ali et al., 2015). Such delay in flowering and maturity is the result of late leaf senescence in wheat and maize due to active photosynthesis (Uzoma et al., 2011; Ali et al., 2015; Ali et al., 2017). Moreover, early booting in BC un-treated plots might be due to the scarcity or shortage of nutrients in these plots which pushed the plants to quickly complete his life cycle. Delayed booting, anthesis and maturity in N treated plots might be due to the enhanced leaf area duration, improved vegetative growth and use efficiency of light with further improvement in use efficiency of N (Sher et al., 2018). Furthermore, delayed in different growth phases due to organic fertilizer sources may be due to the greater concentration of available nutrients throughout the plant life which extended the growth period and ultimately delayed phenology. One of the richest sources of nutrients is PM, which promotes vigorous vegetative growth that further delayed wheat phenology (Jan et al., 2018). Khan et al. (2014) described that too much uptake of N would lead to lengthen maturity stage in plants. Furthermore, delayed maturity in FYM treated plots may possibly be due to maximum availability of nutrients in FYM incorporated plots (Koroto et al., 2017). The change in plant height in BC incorporated plots may possibly be due to the nutritional value of BC. Furthermore, BC had robust attraction for ammonium and nitrate (Atkinson et al., 2010), thus enhanced use efficiency of fertilizer by minimizing loses of nitrogen through leaching (Shi et al., 2020), hence resulted in increased plant height in BC incorporated plots (Zee et al., 2017). Alike result was also obtained by (Sial et al., 2019). Cells protein content increased as the application of N increased and size of plant cell increases, as a result of that greater leaf area (LA), improved photosynthesis rate, more production of assimilates and plant dry matter which eventually make the plant taller (Rahman et al., 2014). Greater availability of both (macro and micro) nutrients and increased WHC of the soil (Ali et al., 2017), as a result fertilized treated plots had more taller plants when compared to fertilizer un-treated or lower fertilized treated plots. Emmanuel (2018); Abbas et al. (2012) described that increasing level of FYM and PM fed the crop in initial as well as in later stage, thus better vegetative growth and development resulted in maximum plant height. Similarly, application of BC increases LA and leaf area index (LAI) of wheat as compared to no BC treated plots. Because BC as a soil amendment strongly attract ammonium and nitrate ion thus reduced its leaching and making it more available in the rahizosphere, thus increased vegetative growth which ultimately improved LA and LAI (Salim, 2016). Parallel results were also achieved by (Batool et al., 2015; Akhtar et al., 2015). The better LA and LAI in N nourished plots may probably be due to the delayed leaf senescence, persistent, and continuous leaf photosynthesis (Sadur et al., 2010), which further increased number of tillers and size of the successive leaves (Ruisi et al., 2015). Moreover, the improved LA and LAI in PM amended plots might be due to the higher consumption of nutrients released through the decomposition of PM, which led to enhanced chlorophyll content, captured maximum solar radiation, increased net photosynthesis and cell division in the leaves (Kareem et al., 2017; Ahmed et al., 2017; Bashir et al., 2017). Similarly, the improved LA of wheat in FYM amended plots might be due to the enhanced N availability and its uptake (Singh et al., 2019). Tillers m-2 of wheat was considerably enhanced by BC and N management. Because the enhanced soil physical properties were expected to benefit wheat tillering and root elongation (Oussible et al., 1993), and therefore improved the wheat vegetative growth with higher tiller number. In addition, BC itself might also provide available phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg) etc., having the potential to boost soil fertility (Silber et al., 2010). The above statement was also supported by (Zhang et al., 2013). The maximum number of tillers production in fertilized plots may possibly be due to the sufficient N availability (Lee et al., 2017), which caused in enhanced photosynthetic activities (Guo et al., 2019), vigorous plant growth (Belete et al., 2018) and eventually more productive tillers. Further, the increased number of tillers in organic amendment plots (especially PM) may possibly be due to the improvement in soil fertility; organic matter, microbial activities and improved soil structure (Mukhtiar et al., 2018). Moreover, Enujeke (2013) stated that incorporation of PM improved plant growth due to the availability of more moisture and nutrients (Abbasi and Khaliq, 2016), leading to bumper growth and development (Jan et al., 2018), and eventually more tillers m-2 (Rasul et al., 2015). Spikes (fertile tillers) contribute directly to the yield of crop. Incorporation of BC had an encouraging influence on it. This might be due to soil reclamation properties of BC, which enhance fertility and use efficiency of nutrients (Negussie et al., 2012). Furthermore, Rahim et al. (2019) observed that application of BC enhanced soil WHC, total N content in the soil and encouraged crop growth, development and improved yield attributes through enhanced physio-chemical properties of the soils. Similarly, Olmo et al. (2016) also counted more spikes of wheat, may possibly be due to the greater nutrient uptake in BC practiced plots. Moreover, N-treated plots produced more spikes when compared to control (Mukhtiar et al., 2018), this might be due to the sufficient N, timely available which may influence the tillering power of the plants and bring about significant changes in number of spikes per plant (Yang et al., 2019). Among the N sources, organic manures are the exceptional sources of nutrients supply to crop plants (Timsina, 2018). Because Organic manures like PM and FYM decomposed slowly, improved water and nutrients retaining ability of the soil and supply nutrients throughout the plant growth (Ahmed et al., 2017), resulted in more number of spikes (Khan et al., 2018; Ali et al., 2011).

 

Conclusions and Recommendations

Biochar (BC) incorporation into soil improved wheat growth over control regardless of the nitrogen (N) sources used. However, organic sources like PM and FYM performed significantly better than mineral N sources (urea) at both with and without BC application. Thus, N should be applied as organic sources along with BC for improving wheat growth under alkaline calcareous soil in echo friendly manner.

 

Novelty Statement

In alkaline calcareous soils the exogenously applied nitrogen (N) as mineral fertilizers is lost in many ways. We assumed that, application of N as organic manures could be a best way for reducing N losses in alkaline soils. Furthermore, Biochar incorporation into such soils may relief this problem directly through nutrients addition and/or indirectly through nutrients retention, because biochar had strong attraction for ammonium ion released by N fertilizers. In this study we tried to evaluate the potential of biochar in enhancing N availability from different N sources and improving crop growth under alkaline calcareous soils.

 

Author’s Contributions

The present work is part of the Mr. Mushtaq Ahmad Khan, PhD study. The scholar is thankful to his major supervisor, Dr. Abdul Basir for planning the experiments and overall supervision during the Ph.D. study. The scholar is also thankful to his co-supervisor Dr. Beena Seed for helping during the study.

Conflict of interest

The authors have declared no conflict of interest.

 

References

Abbas, G., J.Z.K. Khattak, A. Mir, M. Ishaque and H. Hussain. 2012. Effect of organic manures with recommended dose of NPK on the performance of wheat. J. Anim. Plant Sci., 22: 683-687.

Abbasi, M.K. and A. Khaliq. 2016. Nitrogen mineralization of a loam soil supplemented with organic–inorganic amendments under laboratory incubation. Front. Plant Sci. ,1038 (7): 1-9. https://doi.org/10.3389/fpls.2016.01038

Abid, M., Z. Tian, S.T. Ata-Ul-Karim, Y. Cui, Y. Liu, R. Zahoor and T. Dai. 2016. Nitrogen nutrition improves the potential of wheat (Triticum aestivum L.) to alleviate the effects of drought stress during vegetative growth periods. Front. Plant Sci.,  981(7): 1-14. https://doi.org/10.3389/fpls.2016.00981

Agbede, T.M., 2019. Influence of five years of tillage and poultry manure application on soil properties and ginger (Ziginber officinale Roscoe) productivity. J. Crop Sci. Biotech., 22(2): 91-99. https://doi.org/10.1007/s12892-018-0155-0

Agegnehu, G., M.I. Bird, P.N. Nelson and A.M. Bass. 2015. The ameliorating effects of biochar and compost on soil quality and plant growth on a Ferralsol. Soil Res., 53(1): 1-12. https://doi.org/10.1071/SR14118

Ahmed, B.A.M., A.A. Ishag, M.K. Hassan and M.O. Ahmed. 2017. Response of two wheat cultivars (Triticum aestivum L.) to amended nitrogen fertilizer on yield and grain quality in Halfa Elgadidah area. Mol. Biol. Med., 1(5): 133-136. https://doi.org/10.15406/mojbm.2017.01.00029

Akhtar, S.S., M.N. Andersen and F. Liu. 2015. Residual effects of biochar on improving growth, physiology and yield of wheat under salt stress. Agric. Water Manage., 158: 61–68. https://doi.org/10.1016/j.agwat.2015.04.010

Ali, K., F. Munsif, I. Uddin, A. Khan and N. Khan. 2012. Maize penology as affected by tillage practices and nitrogen sources. Agric. Sci. Res. J., 2(8): 453-458.

Ali, K., F. Munsif, M. Zubair, Z. Hussain, M. Shahid, I.U. Din and N. Khan. 2011. Management of organic and inorganic nitrogen for different maize varieties. Sarhad J. Agric., 27(4): 525-529.

Ali, K., M. Arif, F. Shah, A. Shehzad, F. Munsif, I.A. Mian and A.A Mian. 2017. Improvement in maize (Zea mays L) growth and quality through integrated use of biochar. Pak. J. Bot., 49(1): 85-94.

Ali, K., M. Arif, S. Shah, Z. Hussain, A. Ali, S. Munir and H. Sher. 2015. Effect of organic and inorganic nutrients sources on phenology and growth of wheat. Pak. J. Bot., 47(6): 2215-2222.

Arafat, Y., M. Shafi, M.A. Khan, M. Adnan, A. Basir, M. Arshad and J.A. Shah. 2016. Yield response of wheat cultivars to zinc application rates and methods. Pure Appl. Biol., 5(4): 1260-1270. https://doi.org/10.19045/bspab.2016.50151

Arif, M., F. Jalal, M.T. Jan and D. Muhammad. 2014. Integration of biochar and legumes in summer gap for enhancing productivity of cereal based cropping system. Sarhad J. Agric., 30(4): 393-403. https://doi.org/10.1080/21683565.2014.996696

Arif, M., K. Ali, M.S. Haq and Z. Khan. 2013. Biochar, FYM and nitrogen increases weed infestation in wheat. Pak. J. Weed Sci. Res., 19(4): 411-418.

Atkinson, C.J., J.D. Fitzgerald and N.A. Hipps. 2010. Potential mechanisms for achieving agricultural benefits from biochar application to temperate soils: a review. Plant Soil., 337: 1–18. https://doi.org/10.1007/s11104-010-0464-5

Baronti, S., F.P. Vaccari, F. Miglietta, C. Calzolari, E. Lugato, S. Orlandini, R. Pini, C. Zulian and L. Genesio. 2014. Impact of biochar application on plant water relations in Vitis vinífera (L.). Eur. J. Agron., 53(3): 38–44. https://doi.org/10.1016/j.eja.2013.11.003

Bashir, M.U., S.A. Wajid, A. Ahmad, M. Awais, M.A.S. Raza, G.M. Tahir, U. Saeed, M.H.U. Rehman, M. Waqas and S. Abbas. 2017. Irrigation scheduling of wheat at different nitrogen levels in semi-arid region. Turk. J. Field Crops, 22(1): 63-70. https://doi.org/10.17557/tjfc.303880

Basir, A., A. Tahir, K. Afridi, S. Fahad, Z. Ahmad, M. Adnan, M. Alam, S. Shah, A. Khan, F. Wahid, M. Ibrahim, Inayat-ur-Rahman, M.A. Khan and R. Ali. 2018. Optimization of sowing time and seed rates can enhance wheat yield in semi-arid environment. Philipp. Agric. Sci., 101(4): 326-332.

Batool, A., S. Taj, A. Rashid, A. Khalid, S. Qadeer, A.R. Saleem and M.A. Ghufran. 2015. Potential of soil amendments (Biochar and Gypsum) in increasing water use efficiency of Abelmoschus esculentus L. Moench. Front. Plant. Sci.,  733(6): 1-13. https://doi.org/10.3389/fpls.2015.00733

Belete, F., N. Dechassa, A. Molla and T. Tana. 2018. Effect of split application of different N rates on productivity and nitrogen use efficiency of bread wheat (Triticum aestivum L.). Agric. Food Secur., 7(1): 92. https://doi.org/10.1186/s40066-018-0242-9

Blake, G.R. and K.H. Hartge 1986. Bulk density. (in) methods of soil analysis, Part I (2nd Edn), Agronomy Monograph. Klute A (Ed.). ASA and SSSA, Madison. 9: 363–375. https://doi.org/10.2136/sssabookser5.1.2ed.c13

Borisjuk, N., O. Kishchenko, S. Eliby, C. Schramm, P. Anderson, S. Jatayev and Y. Shavrukov. 2019. Genetic modification for wheat improvement: from transgenesis to genome editing. BioMed. Res. Int., https://doi.org/10.1155/2019/6216304

Bremner, J.M. and C.S. Mulvaney. 1982. Nitrogen-Total. In methods of soil analysis. Part 2, Chemical and Microbiological and Properties, 2nd ed.; Page, A.L.; Miller, R.H.; Kenney, D.R., Eds. Am. Soc. Agron. Soil Sci. Soc. Am. Madison, WI, pp. 595–624.

Du, X., M. Xi and L. Kong. 2019. Split application of reduced nitrogen rate improves nitrogen uptake and use efficiency in sweet potato. Sci. Rep., 9(1): 1-11.

Emmanuel, I.O.M.J., 2018. Domestication and responses of wheat (Triticum aestivum L) Growth, yield parameters, quality indices and soil fertility improvement to different organic fertilizers. Curr. Inves. Agric. Curr. Res. 2(3): CIACR. MS. ID, 139. https://doi.org/10.32474/CIACR.2018.02.000139

Enujeke, E.C., 2013. Effects of poultry manure on growth and yield of improved maize in Asaba area of delta state, Nigeria. J. Agric. Vet. Sci., 4(5): 24-30. https://doi.org/10.9790/2380-0452430

Fidel, R.B., D.A. Laird and K.A. Spokas. 2018. Sorption of ammonium and nitrate to biochars is electrostatic and pH-dependent. Sci. Rep., 8(1): 17627. https://doi.org/10.1038/s41598-018-35534-w

Gonzalez-Navarro, O.E., S. Griffiths, G. Molero, M.P. Reynolds and G.A. Slafer. 2016. Variation in developmental patterns among elite wheat lines and relationships with yield, yield components and spike fertility. Field Crop Res., 196: 294-304. https://doi.org/10.1016/j.fcr.2016.07.019

Guo, Z., J. Han, J. Li, Y. Xu and X. Wang. 2019. Effects of long-term fertilization on soil organic carbon mineralization and microbial community structure. PLoS One, 14(1): e0211163. https://doi.org/10.1371/journal.pone.0211163

Hammad, H.M., A. Khaliq, A. Ashfaq, M. Aslam, A.H. Malik, W. Farhad and K.Q. Laghari. 2011. Influence of different organic manures on wheat productivity. Int. J. Agric. Biol., 13(1): 137-140.

Hammad., H.M., A. Khaliq, A. Ahmad, M. Aslam, T. Khaliq, S.A. Wajid, A. Hussain, M. Usman, W. Nasim, W. Farhad and R. Sultana. 2010. Influence of organic manures on weed dynamics and wheat productivity under low rainfed area. J. Crop Environ., 1(1): 13-17.

Haroon, M., F. Idrees, H.A. Naushahi, R. Afzal, M. Usman, T. Qadir and H. Rauf. 2019. Nitrogen use efficiency: farming practices and sustainability. J. Exp. Agric. Int., pp. 1-11. https://doi.org/10.9734/jeai/2019/v36i330235

Holland, B.L., N.A. Monk, R.H. Clayton and C.P. Osborne. 2019. A theoretical analysis of how plant growth is limited by carbon allocation strategies and respiration. In silico Plants, 1(1): diz004. https://doi.org/10.1093/insilicoplants/diz004

Ihsan, M., G. Watson and C.R. Abbiss. 2016. What are the physiological mechanisms for post-exercise cold water immersion in the recovery from prolonged endurance and intermittent exercise? Sports Med., 46(8): 1095-1109. https://doi.org/10.1007/s40279-016-0483-3

Jalal, F., M. Arif, K. Ali, F. Munsif, M.J. Khan and B. Khan. 2016. Integration of biochar and legumes in summer gap for enhancing productivity of wheat under cereal based cropping system. Pak. J. Bot., 48(6): 2281-2288.

Jan, M.F., M.D. Ahmadzai, W. Liaqat, H. Ahmad and W. Rehan. 2018. Effect of poultry manure and phosphorous on phenology, yield and yield components of wheat. Int. J. Curr. Microbiol. App. Sci., 7(5): 3751-3760. https://doi.org/10.20546/ijcmas.2018.704.422

Kareem, I., O.B. Jawando, E.K. Eifediyi, W.B. Bello and Y. Oladosu. 2017. Improvement of growth and yield of maize (Zea mays L.) by poultry manure, maize variety and plant population. Cercet. Agron. Moldova., 50(4): 51-64. https://doi.org/10.1515/cerce-2017-0035

Khan, A., M.T. Jan, M. Arif, K.B. Marwat and A. Jan. 2008. Phenology and crop stand of wheat as affected by nitrogen sources and tillage systems. Pak. J. Bot., 40(3): 1103-1112.

Khan, F., S. Khan, S. Fahad, S. Faisal, S. Hussain, S. Ali and A. Ali. 2014. Effect of different levels of nitrogen and phosphorus on the phenology and yield of maize varieties. Am. J. Plant Sci., 5(17): 2582-2590. https://doi.org/10.4236/ajps.2014.517272

Khan, M.A., A. Basir, M. Adnan, A.S. Shah, M. Noor, A. Khan, J. A. Shah, Z. Ali and A. Rahman. 2017. Wheat phenology and density and fresh and dry weight of weeds as affected by potassium sources levels and tillage practices. Pak. J. Weed Sci. Res., 23(4): 451-462. https://doi.org/10.28941/23-4(2017)-8

Khan, T.U., M.T. Jan, A. Khan, G. Ahmad, M. Ishaq, K. Afridi and M. Saeed. 2018. Integrated management of fertilizer nitrogen and poultry manure enhances wheat production. Pak. J. Agric. Res., 31(3): 207-215. https://doi.org/10.17582/journal.pjar/2018/31.3.207.215

Khattak, S.I., M.S. Baloch, K. Naveed and E.A. Khan. 2017. Improving farmer’s income and nitrogen use efficiency of dry land wheat through soil and foliar application of N-fertilizer. Sarhad J. Agric., 33(3): 344-349. https://doi.org/10.17582/journal.sja/2017/33.3.344.349

Koroto, S., D. Tana and D. Gedamu. 2017. Effect of farmyard manure and mineral NP fertilizers on yield related traits and yield of potato Solanum tuberosum L. at Areka, Southern Ethiopia (Doctoral dissertation, Doctoral Dissertation, Haramaya University).

Lee, M.S., A. Wycislo, J. Guo, D. Lee and T. Voigt. 2017. Nitrogen fertilization effects on biomass production and yield components of Miscanthus× giganteus. Front Plant Sci., 8: 544. https://doi.org/10.3389/fpls.2017.00544

Liu, Z., F. Gao, J. Yang, X. Zhen, Y. Li, J. Zhao and X. Li. 2019. Photosynthetic characteristics and uptake and translocation of nitrogen in peanut in a wheat–peanut rotation system under different fertilizer management regimes. Front. Plant Sci., pp. 10. https://doi.org/10.3389/fpls.2019.00086

Maqsood, M.A., U.K. Awan, T. Aziz, H. Arshad, N. Ashraf and M. Ali. 2016. Nitrogen management in calcareous soils: problems and solutions. Pak. J. Agric. Sci., 53(1): 79-95. https://doi.org/10.21162/PAKJAS/16.4867

Mathan, J., J. Bhattacharya and A. Ranjan. 2016. Enhancing crop yield by optimizing plant developmental features. Development, 143(18): 3283-3294. https://doi.org/10.1242/dev.134072

McLean, E.O., 1982. Soil pH and lime requirement. In Page, A.L., R.H. Miller and D.R. Keeney (eds.) Methods of soil analysis. Part 2 - Chemical and microbiological properties. (2nd Ed.). Agronomy, 9:199-223.

Mierzwa-Hersztek, M., K. Wolny-Koładka, K. Gondek, A. Gałązka and K. Gawryjołek. 2019. Effect of co-application of biochar and nutrients on microbiocenotic composition, dehydrogenase activity index and chemical properties of sandy soil. Waste Biomass Valori. pp. 1-13. https://doi.org/10.1007/s12649-019-00757-z

Mukhtiar, A., A. Waqar, M.K. Khalil, M. Tariq and S. Muhammad. 2018. Evaluating the potential organic manure for improving wheat yield and quality under agro-climatic conditions of Pakistan. Adv. Crop Sci. Tech. 6(2): 349.

Nelson, D.W., and L.E. Sommers. 1982. Total carbon, organic carbon and organic matter. In: Methods of soil analysis (Ed. A.L. Page). Part 2. Agronomy Monographs 9. ASA and SSSA, Madison. WI. pp. 539-579.

Nigussie, A., E. Kissi, M. Misganaw and G. Ambaw. 2012. Effect of biochar application on soil properties and nutrient uptake of lettuces (lactuca sativa) grown in chromium polluted soils. American Eurasian J. Agric. Environ. Sci. 12 (3): 369-376.

Olmo, M., A.M. Lozano, V. Barrón and R. Villar. 2016. Spatial heterogeneity of soil biochar content affects soil quality and wheat growth and yield. Sci. Total Environ. (562): 690-700. https://doi.org/10.1016/j.scitotenv.2016.04.089

Oussible, M., R.R. Allmaras, R.D. Wynch and R.K. Crookston. 1993. Subsurface compaction effects on tillering and nitrogen accumulation in wheat. Agron. J. 85: 619e625. https://doi.org/10.2134/agronj1993.00021962008500030019x

Rahim, H.U., I.A. Mian, M. Arif, Z.U. Rahim, S. Ahmad, Z. Khan and M. Haris. 2019. Residual effect of biochar and summer legumes on soil physical properties and wheat growth. Pure Appl. Bio. 8(1): 16-26.

Rahman, M.Z., M.R., Islam, M.T. Islam and M.A. Karim. 2014. Dry matter accumulation, leaf area index and yield responses of wheat under different levels of nitrogen. Bangladesh J. Agric. 7(1): 27-32.

Rasul, G.A.M., S.T. Ahmedand and M.Q. Ahmed. 2015. Influence of different organic fertilizers on growth and yield of wheat. Am. Eur. J. Agric. Envt. Sci. 15(6): 1123-1126.

Rhoades, J.D., 1996. Salinity: Electrical conductivity and total dissolved solids. p. 417-435. In: J.M. Bartels et al. (ed.) Methods of soil analysis: Part 3 Chemical methods. 3rd ed. ASA and SSSA, Madison, WI. Book Series no. 5. https://doi.org/10.2136/sssabookser5.3.c14

Rizwan, M., S. Ali, T. Abbas, M. Adrees, M. Zia-ur-Rehman, M. Ibrahim and R. Nawaz. 2018. Residual effects of biochar on growth, photosynthesis and cadmium uptake in rice (Oryza sativa L.) under Cd stress with different water conditions. J. Environ. Manage. 206: 676-683.

Ruisi, P., B. Frangipane, G. Amato, A.S. Frenda, A. Plaia, D. Giambalvo and S. Saia. 2015. Nitrogen uptake and nitrogen fertilizer recovery in old and modern wheat genotypes grown in the presence or absence of inters pecifc competition. Front. Plant Sci. 6: 1–10. https://doi.org/10.3389/fpls.2015.00185

Ryan, J., G. Estefan and A. Rashid. 2001. Soil and plant analysis laboratory manual (2nd ed.). Jointly published by the International Center for Agricultural Research in the Dry Areas (ICARDA) and the National Agricultural Research Center (NARC). Available from ICARDA, Aleppo, Syria. pp.172.

Sadur, R., S.K. Khalil, M. Fida, R. Abdur, A.Z. Khan, A.R. Saljoki and I.H. Khalil. 2010. Phenology, leaf area index and grain yield of rainfed wheat influenced by organic and inorganic fertilizer. Pak. J. Bot. 42(5): 3671-3685.

Salim, B.B.M., 2016. Influence of biochar and seaweed extract applications on growth, yield and mineral composition of wheat (Triticum aestivum L.) under sandy soil conditions. Ann. Agric. Sci. 61(2): 257-265. https://doi.org/10.1016/j.aoas.2016.06.001

Sara, Z.S. and T. Shah. 2018. Residual Effect of Biochar on Soil Properties and Yield of Maize (Zea mays L.) under Different Cropping Systems. Open J. Soil Sci. 8(01): 16-35. https://doi.org/10.4236/ojss.2018.81002

Sawan, Z.M., 2013. Plant growth retardants, plant nutrients, and cotton production. Commun. Soil Sci. Plan. 44(8): 1353-1398. https://doi.org/10.1080/00103624.2012.756509

Sher, A., K. Naveed, G. Ahmad, A. Khan, M. Saeed and S. Masaud. 2018. Phenology and Biomass Production of Wheat in Response to Micronutrients and Nitrogen Application. Sarhad J. Agric. 34(4): 712-723. https://doi.org/10.17582/journal.sja/2018/34.4.712.723

Shi, W., Y. Ju, R. Bian, L. Li, S. Joseph, D.R. Mitchell and G. Pan. 2020. Biochar bound urea boosts plant growth and reduces nitrogen leaching. Sci. Total Environ., 701: 134424. https://doi.org/10.1016/j.scitotenv.2019.134424

Sial, T.A., Z. Lan, L. Wang, Y. Zhao, J. Zhang, F. Kumbhar and A.N. Shah. 2019. Effects of different biochars on wheat growth parameters, yield and soil fertility status in a silty clay loam soil. Molecules, 24(9): 1798. https://doi.org/10.3390/molecules24091798

Silber, A., I. Levkovitch and E.R. Graber. 2010. pH-dependent mineral release and surface properties of corn straw biochar: Agronomic implications. Environ. Sci. Technol., 44: 9318e9323. https://doi.org/10.1021/es101283d

Silva, I.C.B.D., J.J.N. Basílio, L.A. Fernandes, F. Colen, R.A. Sampaio and L.A. Frazão. 2017. Biochar from different residues on soil properties and common bean production. Sci. Agricola., 74(5): 378-382. https://doi.org/10.1590/1678-992x-2016-0242

Singh, P., V.K. Agrawal and Y.V. Singh. 2019. Effect of potassium and FYM on growth parameters, yield and mineral composition of wheat (Triticum aestivum L.) in alluvial soil. J. Pharmacogn. Phytochem, 8(3): 24-27. https://doi.org/10.1080/00103627709366714

Soltanpour, P.N. and A.P. Schwab. 1977. A new soil test for simultaneous extraction of macro-and micro-nutrients in alkaline soils. Commun. Soil Sci. Plant Anal., 8(3): 195-207.

Steel, R.G.D. and J.H. Torri. 1980. Principal and Procedures of Statistics. New York: McGraw HillBook Co.

Stumpf, B., F. Yan and B. Honermeier. 2019. Influence of nitrogen fertilization on yield and phenolic compounds in wheat grains (Triticum aestivum L. ssp. aestivum). J. Plant Nutr. Soil Sci., 182(1): 111-118. https://doi.org/10.1002/jpln.201800342

Timsina, J., 2018. Can organic sources of nutrients increase crop yields to meet global food demand? Agronomy, 8(10): 214. https://doi.org/10.3390/agronomy8100214

Uzoma, K.C., M. Inoue, H. Andry, H. Fujimaki, A. Zahoor and E. Nihihara. 2011. Effect of cow manure biochar on maize productivity under sandy soil condition. Soil Use Mgt., 27(2): 205-212. https://doi.org/10.1111/j.1475-2743.2011.00340.x

Wang, Y., F. Pan, G. Wang, G. Zhang, Y. Wang, X. Chen and Z. Mao. 2014. Effects of biochar on photosynthesis and antioxidative system of Malus hupehensis Rehd. Seedlings under replant conditions. Sci. Hortic., 175: 9–15. https://doi.org/10.1016/j.scienta.2014.05.029

Yang, D., T. Cai, Y. Luo and Z. Wang. 2019. Optimizing plant density and nitrogen application to manipulate tiller growth and increase grain yield and nitrogen-use efficiency in winter wheat. PeerJ, 7: e6484. https://doi.org/10.7717/peerj.6484

Zee, T.E., N.O. Nelson and G. Newdigger. 2017. Biochar and nitrogen effects on winter wheat growth. Kan. Agric. Exp. St. Res. Rep., 3(3): 1-6. https://doi.org/10.4148/2378-5977.1397

Zhang, H., L. Zhang, Y. Huang, Y. Ye, J. Cui and S. Eldad. 2013. Effect of potassium application on yield and soil potassium balance in maize and wheat rotation system. J. Triticeae Crops, 33: 988e995.

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Sarhad Journal of Agriculture

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Pakistan J. Zool., Vol. 56, Iss. 6, pp. 2501-3000

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