Biochemical Characterization of Bacterial Pathogens from Corneal Eye Infections and their Control via Chemotherapy, Phytotherapy and Apitherapy
Biochemical Characterization of Bacterial Pathogens from Corneal Eye Infections and their Control via Chemotherapy, Phytotherapy and Apitherapy
Nazish Mazhar Ali1, Asma Ashraf 2*, Iram Liaqat2, Bushra Mazhar2, Shaukat Ali2 and Saiqa Andleeb3
Nazish Mazhar Ali1, Asma Ashraf 2*, Iram Liaqat2, Bushra Mazhar2, Shaukat Ali2 and Saiqa Andleeb3
ABSTRACT
The present study was conducted to isolate and characterize pathogenic bacteria from eye infections. The samples were taken from corneal eye infections. The collection of samples was done from “Layton Rehmat Eye Hospital, Township Lahore”. Isolated bacterial pathogens were identified on the basis of morphological and various biochemical tests. Morphologcal tests included different staining procedures includng Gram’s staining and Endospore staining. Identification of pathogenic bacterial isolates was confirmed by growing on selective medium. Antibacterial activity of different compounds was observed against the pathogenic bacterial isolates. Minimum Inhibitory Concentration (MIC) test of different kinds of honey was performed against the isolated bacterial pathogens. The antibacterial activity of leaf extract of Azadirachta indica (Neem Plant) was also checked against the pathogenic bacterial isolates. The bacterial pathogens isolated from eye infections were identified as Staphylococcus sp., Streptococcus sp., Neisseria sp., and Bacillus sp. These bacteria commonly caused corneal infections. All the pathogenic bacterial isolates showed sensitivity against all the compounds except Streptococcus sp. (BS1) and it showed resistance only against 3-Nitro-aniline (SAHC-11). Bacterial isolate Staphylococcus sp. (BS4c) showed maximum sensitivity against methyl-amine (SAH-5) with zone of inhibition of about 16mm in diameter while the bacterial isolate Staphylococcus sp. (BS6) showed minimum sensitivity against Propyl-diamine (SAH-16) and Ethylamine cinnamaldehyde derivative (SAHC-7) compounds with 1mm diameter of zone of inhibition under optimum growth conditions i.e., optimum pH and temperature. Antibacterial activity of leaf extract of Azadirachta indica (Neem Plant) was also checked. Methanol is used as a standard against which all the pathogenic bacterial isolates showed resistance. The minimum sensitivity showed by Staphylococcus sp. (BS4C) with the zone of inhibition of about 2mm in diameter while the maximum sensitivity showed by Staphylococcus sp. (BS6) with zone of inhibition of 8mm. MIC test of four different kinds of honey was also performed. Different honeys showed different results. For all the isolated bacterial pathogens, Pak honey inhibited the growth at 1ml concentration, Local honey at 3ml concentration, Marhaba’s honey at 4ml concentration, and Young’s honey inhibited the growth at the concentration of 5ml.
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June 2019
Vol. 5, Iss. 1, Pages 1-52
