Cellular Localization of MAPK, NF-κB and Nrf2 Signaling Pathways-Related Proteins in Crayfish Hepatopancreas
Keqiang Wei1*, Yue Wei2 and Changxia Song1
1School of Life Science, Shanxi University, Taiyuan 030006, China
2College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China
Fig. 1.
Localization and distribution of signaling pathway-related proteins in hepatopancreas of red swamp crayfish (P. clarkii). A, H and E staining; B, negative control (no primary antibody); C and D, p38MAPK; E and F, p-p38 MAPK; G and H, RelA (p65); I and J, Nrf2. The representative immunopositive cells were indicated with red arrowheads. Square indicated partial enlarged view. B-cell, secretory cell; BT, blind tubule; CT, connective tissue; ER, epithelium; HS, haemolymph sinusoids; R-cell, absorptive cell. 100 ×; scale bars=50 μm.
Fig. 2.
Tissue distribution of signaling pathway-related proteins in hepatopancreas of red swamp crayfish (P. clarkii). Expression levels were quantified as mean optical density (MOD) value of the immunopositive cells. Data were expressed as the mean ± SD (n = 6).