Characterization of Celery Mosaic Virus Isolated from Some Apiaceae Plants
Characterization of Celery Mosaic Virus Isolated from Some Apiaceae Plants
Ahmed, AmalA.; Zein, Salwa N. and Khatab, Eman A. H.
ABSTRACT
Celery mosaic Potyviruses (CeMV) was detected and isolated from naturally infected celery plants showing mosaic symptoms suspected to be caused by viral infection, also, it was isolated from other hosts. The isolated virus was biologically purified from single local lesions formed on Chenopodium amaranticolor. CeMV was identified by its host range, symptom expression, modes of transmission and particle morphology. CeMV able to infect only 20 plant species and varieties from 22 tested by mechanical inoculation. The virus was transmitted in a non-persistent manner using Myzus persicae Sulz. Infected plants were reacted positively only with the specific antiserum for the CeMV using double antibodies sandwich—enzyme linked immunosorbent assay (DAS-ELISA). It was successfully purified from infected N. tabacum L. var. White Burley leaves and virus particles had filamentous flexuous shape. Only one band of purified virus preparation was observed 1.5 cm bellow the meniscus of the density — gradient column. Infectivity test of the viral zone was found positive. The absorption spectrum of the purified virus isolate had a maximum at 260 nm and a minimum at 243 nm. The max/min and A260/280 ratios of the virus isolate were 1.12 and 0.96, respectively. The yield of purified -virus was 6.7 mg/100g of tobacco leaves. Electron micrographs of the purified virus isolate revealed the presence of filamentous particles with average length of 780 nm and 15 widths. The polyclonal antibody raised against the virus under study had a virus — specific titer of 1:7500 in bled one and 1: 7000 in bled two. Incidence of the disease reached 75% in some apiaceae crops and many of the infected plants were unfit for marketing. It is suggested that control of the disease may be achieved by introducing a celery-free period to break the cycle of virus transmission between successive crops. This work records of the first time the prescience of this disease in Egypt.
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