Clinical Significance and Correlation of CXCL8 and its mRNA in the Children with Mycoplasmal Pneumonia
Clinical Significance and Correlation of CXCL8 and its mRNA in the Children with Mycoplasmal Pneumonia
Lin Sun1, Xiaofang Bu2, Jian Wang1*, Xiaorui Liu1 and Zhanyi Kong2
ABSTRACT
The acute inflammation in bronchial and pulmonary interstitial are regarded as the main pathological injury of Mycoplasma pneumoniae pneumonia (MPP). However, the exact mechanism of CXCL8 and its mRNA in the children with Mycoplasmal pneumonia needs to be further clarified. The concentration of the CXCL8 in serum and the level of CXCL8 mRNA in PBMCs of forty-eight children were dynamically measured by ELISA and PCR. The ratio of lgcDNA/lgGAPDH was regarded as the extreme level of CXCL8 mRNA. The serum level of CXCL8 and expression of CXCL8 mRNA in PBMCs in MPP children were (298.917±51.860) pg/mL and (1.848±0.525) lgcDNA/lgGAPDH. There were significant differences between the trial groups and normal controls (P<0.05). Further observation showed that the levels of CXCL8 mRNA in peripheral blood of the children with severe illness were significantly higher than those in light cases (P<0.05). Intravenous infusion of Erythromycin was provided in the acute phase for seven to ten days, and followed by the use of sequential therapy of Azithromycin for about three to four weeks, the children’s condition were gradually from acute stage to recovery stage. CXCL8 and its mRNA levels in peripheral blood of the sick children were all significantly decreased comparing with those in the acute stage (P<0.05). Further analysis showed that CXCL8 and its mRNA had a significant correlation in the acute phase, and it was related to the severity of the disease and the course of the disease. The correlation between CXCL8 and its mRNA was significantly decreased in the recovery phase. There was only a weak correlation between serum CXCL8 level and serum Anti-MP level in both acute and convalescent stages (r= -0.2917, P=0.0891; r=-0.2783, P=0.1055). The level of CXCL8 and its mRNA was increased in the peripheral blood of the sick children with mycoplasma pneumonia, and also correlated with the severity of the disease. The level of CXCL8 in peripheral blood was strongly correlated with its mRNA and weakly correlated with anti-MP level. The content of CXCL8 in serum of the sick children can be reduced by Azithromycin via the pathway of inhibiting the proliferation of MP.
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February 2020
Vol. 52, Iss. 1, Pages 1-424
