Detection and Phylogeny of Infectious Bursal Disease Virus (IBDV) during Field Outbreaks in Broilers
Detection and Phylogeny of Infectious Bursal Disease Virus (IBDV) during Field Outbreaks in Broilers
Umm-i-Habiba1,*, Ayesha Maqbool2, Muhammad Safdar3, Nida Zia1, Altaf Mehmood4, Muhammad Usman5, Mehwish Sharif6, Amanullah Khan7 and Sajid Umar8
Amplification product of VP2 gene from IBDV. L, DNA marker; Lanes 3 to 8, positive samples; PC, positive control; NC, negative control.
Phylogenetic tree based on the VP2 gene sequences of IBDV. IBDV detected in present study have been represented with black circles.
VP2 protein based analysis showing amino acid pattern of IBDV. The amino acid alanine at the position 222 and other amino acids I242, I256, I294 and S299 is characteristic for vvIBDV.