Detection of Mycoplasma bovis from Cattle Presented for Slaughter in Adamawa and Taraba States, Northeastern Nigeria
Markus I. Francis1*, Paul I. Ankeli2, Clara N. Kwanashie1, Jibril Adamu1, Lushaikyaa Allam1, Mashood A. Raji3, Godwin O. Egwu4, Flavio Sacchini5 and Massimo Scacchia5
Map of Adamawa and Taraba States, North-eastern Nigeria (MLS, 2010).
Colonies of Mycoplasma bovis from lung sample on PPLO agar showing ‘fried egg’ colonies with nipples ‘n’ and raised transparent periphery ‘t’ observed under Stereomicroscope (X40).
PCR amplification of Mycoplasma bovis 16S rRNA gene on 1% agarose gel with primers MBOF2 and MBOR2. M: molecular marker of 50-bp; PC: positive control reference strain PG45 (734-bp); NC: negative control; Lanes 1-5: represents samples TL41, TL43, TL46, TES57 and AP106.