Diversity of Extended Spectrum β-lactamases among Multi Drug Resistant Clinical Isolates of Pseudomonas aeruginosa Collected fromTertiary Care Hospitals of Peshawar, Pakistan
Diversity of Extended Spectrum β-lactamases among Multi Drug Resistant Clinical Isolates of Pseudomonas aeruginosa Collected fromTertiary Care Hospitals of Peshawar, Pakistan
Amjad Ali, Kafeel Ahmad*and Shaista Rahat
Double Disc Synergy Test: ESBL positive Pseudomonas aeruginosa, the size of zones of inhibition around cefepime, ceftazidime and aztreonam are enhanced towards center amoxicillin-clavulanic acid disc as shown by arrows.
A, PCR amplification of blaOXA-10 gene (A) Lane 1 = 100 bp DNA ladder. Lanes 4, 5, 8, 12, 13, 14 and 16 in A show blaOXA-10 (760 bp) blaCTX-M gene; (B), Lanes 3, 8, 11, 12, 13, 15 and 16 in B show blaCTX-M (552 bp) positive blaTEM gene (C), Lanes 3 and 16 show blaTEM (856 bp) positive blaSHV gene and (D) Lanes 2, 3 and 4 in D show blaSHV (230 bp) positive Pseudomonas aeruginosa isolates.
A, PCR amplification of blaOXA-10 gene (A) Lane 1 = 100 bp DNA ladder. Lanes 4, 5, 8, 12, 13, 14 and 16 in A show blaOXA-10 (760 bp) blaCTX-M gene; (B), Lanes 3, 8, 11, 12, 13, 15 and 16 in B show blaCTX-M (552 bp) positive blaTEM gene (C), Lanes 3 and 16 show blaTEM (856 bp) positive blaSHV gene and (D) Lanes 2, 3 and 4 in D show blaSHV (230 bp) positive Pseudomonas aeruginosa isolates.
