This study investigates how the ratio of rumen-degradable protein (RDP) to non-fiber carbohydrates (NFC) in cattle feed affects the production of ammonia (NH3), total volatile fatty acids (VFA), and protozoa. The experimental design was completely randomized (CRD) with six treatments and five replications. The treatments consisted of varying RDP to NFC ratios: R1 (60:35), R2 (60:40), R3 (65:35), R4 (65:40), R5 (55:39), and R6 (55:41). The research data were analyzed for variance, followed by Duncan’s Multiple Range Test. The results indicated that the six treatments had a significant effect (P < 0.05) on NH3 production and total VFA but no significant impact (P > 0.05) on protozoa populations. This study concluded that the ratio of rumen degradable protein (RDP) to non-fiber carbohydrates (NFC) significantly increased the production of ammonia (NH3) and total volatile fatty acids (VFA). However, it did not affect the protozoan population. The treatments R1, R3, and R4 exhibited the highest levels of NH3 and total VFA production.
Keywords | RDP, NFC, NH3, Total VFA, Protozoa, Rumen