Effect of various concentrations of Zinc on Peroxidase activity of Catla catla
Effect of various concentrations of Zinc on Peroxidase activity of Catla catla
MARIUM ASLAM, MUHAMMAD JAVED, FAIZA AMBREEN* & FARIHA LATIF
ABSTRACT
Antioxidant enzymes are the first line of defense against reactive oxygen species (ROS) and other free radicals. When the rate of ROS exceeds the capacity of antioxidant enzymes, non-detoxified radicals begin to attack the bio-molecules. Therefore, present research work was planned to study the effect of zinc on the peroxidase activity in the liver and kidney of Catla catla. To check the concentration based effect, C. catla were exposed, separately, to 96-hr LC50 of zinc and its sub-lethal concentrations viz. 2/3rd, 1/4th and 1/5th of LC50 for 30 days at constant laboratory conditions. After 30-day, the fish were sacrificed and their liver and kidney were analyzed for peroxidase enzyme activity. Change in activity of peroxidase enzyme in the fish exposed to sub-lethal concentrations was compared with the control. Dose dependent increase in the activities of peroxidase enzyme was observed in the tissues of fish as compared to the control group. Peroxidase activity in the liver of fish from all the treatments was significantly (p<0.05) higher than that of kidney. The results of these studies in fish tissues may prove that peroxidase activity can be used as a sensitive bio-indicator of the antioxidant defense system.
Key Words: Catla catla, Sub-lethal, Zinc, Antioxidant Enzyme
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December 2017
Vol.63, Iss. 2, Pages 125-234
