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Efficacy of Dietary Supplementation with Phytase and Citric Acid on Whole-Body Composition and Hematological Parameters of Cyprinus carpio and Cirrhinus mrigala Fingerlings Fed on Canola Meal-Based Diet

Efficacy of Dietary Supplementation with Phytase and Citric Acid on Whole-Body Composition and Hematological Parameters of Cyprinus carpio and Cirrhinus mrigala Fingerlings Fed on Canola Meal-Based Diet

A 90-day feeding trial was designated to evaluate how canola meal (CM)-based diet supplemented with phytase (PHY) and citric acid (CA) affects whole-body composition and hematological parameters in Cirrhinus mrigala and Cyprinus carpio fingerlings. Sixteen experimental diets (T1─T16) with varied CA (0 and 2.5%) and PHY (0 and 750 FTU/kg) levels were prepared. The fingerlings were fed at the rate of 5.0% of live fish body mass. Fifteen fish were randomly stocked into triplicate tanks for each of sixteen test diets. Significant (p<0.05) increase in crude protein (CP) and crude fat (CF) was noticed in fish fed T12 (2.5% CA and 750 FTU/kg PHY) diets. Comparison of treatments showed maximum values of RBCs (3.57×106 mm-3 and 3.18×106 mm-3), PLT (83.32 and 80.35), Hb (8.92g/100ml and 8.62g/100ml) and PCV (29.07% and 30.07%) in C. mrigala and C. carpio fingerlings, respectively, when fed with T12 diets. Conclusively, a 50% CM-based diet with 2.5% CA and 750 FTU/kg PHY supplementation, performed better in terms of whole-body composition and hematological parameters in C. mrigala and C. carpio fingerlings.

1Department of Life Sciences, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan, Pakistan
2Fish Nutrition Lab, Department of Zoology, Government College University, Faisalabad, Pakistan
3Department of Chemistry, Government College University Faisalabad, Pakistan
4Department of Fisheries and Aquaculture, University of Okara, Okara, Pakistan
 
*      Corresponding author: [email protected]

Table IV.

Hematological parameters of C. mrigala fingerlings fed on canola meal-based test diets supplemented with Phytase and citric acid.

Means within rows having different superscripts are significantly different at p<0.05.
Table V.

Hematological parameters of C. carpio fingerlings fed on canola-meal based test diets supplemented with phytase and citric acid.

Means within rows having different superscripts are significantly different at p<0.05.

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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