Establishment and Application of a SYBR Green I Real-Time Quantitative PCR for Detection of Micropterus salmoides Rhabdovirus
Establishment and Application of a SYBR Green I Real-Time Quantitative PCR for Detection of Micropterus salmoides Rhabdovirus
Ningyu Zhu, Runzhen He, Qianrong Liang, Xiaoye Zheng, Gaohua Yao, Wenjun Ma and Xueyan Ding*
Fig. 1.
Standard curve (A) and melting cure (B) of the SYBR Green I RT-qPCR.
Fig. 2.
Sensitivity test results of the PCR (A) and SYBR Green I RT-qPCR (B). M: DL2000 marker, 1: 2.78×107 copies; 2: 2.78×106 copies; 3: 2.78×105 copies; 4: 2.78×104 copies; 5: 2.78×103 copies; 6: 2.78×102 copies; 7: 2.78×101 copies; 8: 2.78×100 copies.
Fig. 3.
Specificity analysis of the SYBR Green I RT-qPCR.
