Evaluation of the Effect of Microoperation on Porcine Parthenogenetic Embryo Competence by Raman Spectroscopy
Evaluation of the Effect of Microoperation on Porcine Parthenogenetic Embryo Competence by Raman Spectroscopy
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
Fig. 1.
Development of porcine parthenogenetic embryos with the different types of microoperation. I, intact oocytes pathenogenesis; II, the puncture of zona and plasma membrane of oocytes parthenogenesis; III, the puncture of zona and plasma membrane and aspiration cytoplasm of oocytes parthenogenesis.
Fig. 2.
The shape of parthenogenetic blastocysts generated from intact oocytes and different microoperated oocytes
Fig. 3.
Raman spectra of the spent culture media from porcine parthenogenetic blastocysts by difference types of microoperation
Fig. 4.
Pairwise subtraction Raman spectra of spent culture media with difference types of microoperation.
Fig. 5.
PCA 3D scatter plots of porcine parthenogenetic blastocysts with difference types of microoperarion.
October 2019
Vol. 51, Iss. 5, Pages 1599-1997
