Explanation of the Intracellular Survival Mechanisms of Babesia Bovis in Bovine Endothelial Cells
Explanation of the Intracellular Survival Mechanisms of Babesia Bovis in Bovine Endothelial Cells
Safa T. Whaeeb1*, Zeid Alsadoon2, Ali Hussein Fadhil3 Hayder M. Mohammed4
ABSTRACT
The current study was conducted with the objective of elucidating the interaction dynamics between Babesia bovis (B. bovis) parasites and Bovine Aorta Endothelial Cells (BAECs), while identifying potential molecular and proteomic changes post-invasion. BAECs were cultured for seven days under constant monitoring. Isolated B. bovis parasites were cultivated and subsequently introduced to BAECs. Post-invasion changes were observed using microscopy, qPCR, and RNA sequencing, with additional proteomic analysis of the cells conducted via mass spectrometry. Data were statistically evaluated using SPSS, with significant differences identified through ANOVA followed by a post-hoc Tukey’s test. The results showed that BAECs reached a growth plateau by day 5. The B. bovis invasion rate was 85% within the first 4 hours. The msa-2b gene and the 18S rRNA gene exhibited differential expression post-invasion. Proteomic analysis revealed upregulation of proteins associated with msa-2b and downregulation of Protein B after invasion. Significant differences were observed between groups (p<0.05). In conclusion, B. bovis demonstrates an aggressive invasive tendency towards BAECs, with distinct molecular and proteomic shifts indicative of adaptive survival mechanisms and potential therapeutic targets.
Keywords | B. bovis, BAECs, Invasion dynamics, Msa-2b gene, Proteomic analysis
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