FK228 Ameliorates the Liver Toxicity and Oxidative Stress on Thiram-Induced Tibial Dyschondroplasia in Chicken
FK228 Ameliorates the Liver Toxicity and Oxidative Stress on Thiram-Induced Tibial Dyschondroplasia in Chicken
Muhammad Kashif Iqbal1*, Khalid Mehmood2,4, Shakeel Ahmed3, Fazul Nabi4, Muhammad Arif Rizwan1, Muhammad Kaleem1, Mushtaq Ahmed1, Abdul Waheed1 and Jiakui Li5
The effect of FK228 on growth-plate width and morphology. Chicks and tibial growth plates were photographed on day 14. Control group with normal growth plate size, thiram-induced tibial dyschondroplasia (TD) group have increased growth plate width while FK228 group restored growth-plate size after treatment. GP, growth plate; TDL, TD lesion.
VEGF mRNA expression was analyzed by quantitative real time polymerase chain reaction (qRT-PCR) on day 10 and 14 in avian growth plate of normal, thiram, FK228 treated avian chicks. Data expressed in arbitrary units as the means±SE (n= 6 for each group). Control group set to one thus equivalent to the N-fold difference. a, b letters designate significant difference (P <0.05).
Gene expression of Flk-1A was analyzed by quantitative real time polymerase chain reaction (qRT-PCR) on day 10 and 14 in avian growth plate of normal, thiram, FK228 treated avian chicks. Data expressed in arbitrary units as the means ± SE (n = 6 for each group). Control group set to one thus equivalent to the N-fold difference. a, b, c letters designate significant difference (P <0.05).
