Faba bean necrotic yellows virus (FBNYV) in Egypt: Characterization and Virus-Vector Relationship
Faba bean necrotic yellows virus (FBNYV) in Egypt: Characterization and Virus-Vector Relationship
A. E. Aboul-Ata, Mohga, M. El-Tahlawey, M. A. Amer and A.M. Mandour
ABSTRACT
Faba bean necrotic yellows virus (FBNYV) has been isolated and identified as an isolate of FBNYV from Egypt. FBNYV characterization was proved according to the following Criteria: Symptomatology, insect transmission (Aphis craccivora), serological tests (DAS-ELISA), PCR product for amplified replicase gene Of FBNYV (920 bp). Virus-vector relationship was recognized as follows: AAP is 2 hr. IAP is 0.5 hr. Latent period in the aphid is 18 hr. and retention period is 18 days. FBNYV was tested for incidence in the FBNYV-virulefirous aphids (A. craccivora) using PCR products of amplified replicase gene of FBNYV (290 bp) It is not trans-ovarially transmitted through new progenies of A craccivora. The virus has been able to be transmitted by five different aphid species i.e. A. craccivora (93.84), A Fabae (89 09), A. Pisum (77 50). A sesbaniea (56.25). and A. gossypii (38.46). The virus transmissibility by aphid is higher parallel to number of insects allowed to acquire the virus. FBNYV is transmitted by Nymphal Stage more than adult Stage (78.7% and 34.3% respectively). FBNYV is being able to be acquired by A. craccivora insects after 4-day post-inoculation period. The virus is not being able to be transmitted throughout newly hatched nymphs without acquiring virus.
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