Gene Expression Analysis of ERF15, WRKY11, and CYP450 in Tobacco Infected with Ralstonia solanacearum
Gene Expression Analysis of ERF15, WRKY11, and CYP450 in Tobacco Infected with Ralstonia solanacearum
Johan Sukweenadhi1,2*, James Setiabudi2, Wina Dian Savitri2 and Se Chan Kang3
ABSTRACT
Bacterial wilt is one of the destructive infectious diseases in tobacco plants (Nicotiana tabacum L.). Ralstonia solanacearum Rsis the bacteria responsible for the incidence of the disease. Crop yields can be dramatically reduced in quantity and quality due to attack by this pathogen. Developing cultivars resistant to Rs is a challenge to overcome this problem. Marker-assisted selection (MAS) plays a critical role as a selection tool in the development of high-yielding cultivars. The current study aims to provide basic information regarding the expression of several genes associated with Rs in tobacco. Four tobacco cultivars, namely Hick Broadleaf, Beinhart-1000, Dark-302, and Dark-314, were artificially infected using Rs and subjected to cDNA analysis using end-point PCR in triplication. Results showed that ERF15, WRKY11, and CYP450 genes showed no change in expression in the tested tobacco cultivars. Further experiments considering these factors could help elucidate the precise mechanisms governing the three genes expression in response to R. solanacearum infection.
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July 2023
Vol. 39, Sp. Iss. 1
