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Genetic Diversity of Mycobacterium tuberculosis Complex Prevailing In Khyber Pakhtunkhwa, Pakistan

Genetic Diversity of Mycobacterium tuberculosis Complex Prevailing In Khyber Pakhtunkhwa, Pakistan

Bashir Ahmad1, Muhammad Idrees1,*, Kafeel Ahmad1, Dawood Ahmad2, Sajid Ali3 and Shumaila Bashir4

1Centre of Biotechnology and Microbiology, University of Peshawar, Peshawar
2Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar
3TB Reference Laboratory, Peshawar, Khyber Pakhtunkhwa
4Department of Pharmacy, University of Peshawar, Peshawar

*      Corresponding author: [email protected]

Fig. 1.

Comparative evaluation of sensitivity of fluorescence microscopy and GeneXpert assay.

Fig. 2.

 Immunochromatographic test for samples 2 to 5 to differentiate MTBC from other mycobacterial species.

Fig. 3.

Dendrogram showing genetic relatedness among MTBC strains.

Fig. 4.

Agarose gel electrophoresis of PCR products. Lane 1, DNA Marker; Lane 2, H37Rv; Lane 3, M. bovis; Lane 4 to 15, MTBC clinical strains with visible amplification at 439.

Fig. 5.

A, Agarose Gel Electrophoresis showing MTBC isolates after digestion with BstEII restriction enzyme. Lane 1, DNA marker; Lane 2, positive control H37Rv; Lane 3, M. bovis; Lane 4 to 16, MTBC clinical strains. B, Agarose Gel Electrophoresis showing MTBC isolates after digestion with HaeIII restriction enzyme. Lane 1, DNA marker; Lane 2, H37Rv; Lane 3, M. bovis; Lane 4 to 10, MTBC clinical strains.

Senstivity
Specificity

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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