Green-Synthesis-Zinc-Oxide-Nanoparticles-Bacillus
Green-Synthesis-Zinc-Oxide-Nanoparticles-Bacillus
Mehwish Iqtedar*, Mahnoor Siddique, Asma Shahzad, Afshan Kaleem and Roheena Abdullah
ABSTRACT
Zinc oxide nanoparticles are widely and safely used due to their characteristic nature. The current study is focused at microbial synthesis of Zinc oxide nanoparticles (ZnO NPs) and their applications in biomedical and environmental areas. Intracellular ZnO NPs were developed utilizing Bacillus subtilis (CP002905.1) which is not only cost-effective but eco-friendly method too. The UV-Vis spectrum of the synthesized ZnO NPs displayed a distinct peak at 275 nm while FTIR analysis exhibited a major peak at 497.18 cm-1 revealing the ZnO bond. Size and zeta potential of ZnO NPs was 451 nm and -47.5 mV, respectively. Nevertheless, SEM revealed nanoflowers assembly of ZnO NPs. The results further disclosed antimicrobial potential of NPs against Staphylococcus aureus (BTCB02), Salmonella typhimurium (BTCB23)and Escherichia coli (BTCB03)at varying intensities and a maximum zone of inhibition (ZOI) of 15 mm was established with S. aureus at 125 µL. Moreover, the ZnO NPs also showed photocatalytic activity of 80.1% against dye (methylene blue). Hence these NPs can be used in various biomedical and environmental applications in days to come.
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