Histopathological and Molecular Characterization of Heterophyids in Fish and Attitude Towards Parasitic Zoonosis among Residents in Kafrelsheikh Governorate, Egypt

Samah Abou Asa1, Omnia Tag2 and Walid Elmonir2*

1Department of Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt.

2Department of Hygiene and Preventive Medicine, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt.

ABSTRACT

Heterophyiasis is a wide-spread yet under-recognized public health issue in Egypt, especially in regions close to major northern Lakes as Kafrelsheikh Governorate, Egypt. The current study aimed to utilize molecular and histopathological techniques to detect encysted metacercariae (EMC) of Heterophyids in mullet (n= 50) and tilapia (n= 50) fish and describe their pathological effect on fish meat quality. Also, knowledge, attitude and practices (KAPs) related to fish-borne parasitic zoonoses among 100 residents in Kafrelsheikh Governorate were also investigated. The overall prevalence of EMC infection in examined fish was 32% (32/100): 44% (22/50) in mullet and 20% (10/50) in tilapia fish samples. The EMC were 3 times more likely detected in mullet fish compared to tilapia fish (OR= 3.1, P= 0.01). The heterophyids EMC were molecularly confirmed in 32% (16/50) and 18% (9/50) of mullet and tilapia fish samples, respectively. The EMC infection in fish was associated with muscle degeneration, fragmentation, and hyalinization, and mononuclear cell infiltrations, were morphological characteristics of EMC-infected muscles. In mullet fish the lesions were more severe and the EMC were embedded in intermuscular fat. These pathological lesions highlight the poor meat quality of infected fish especially mullet species. Only few residents (14%) had knowledge about Heterophyiasis in the study area. Residents also reported many risk practices as consuming fresh without prior freezing (92.7%), salting fish for less than 10 days (50%), and cooking fish for less than 10 min (2.3%). Health education for residents and infection control in fish are mandate preventive measures in the study area.

Article Information

Received 02 August 2022

Revised 06 August 2022

Accepted 10 August 2022

Available online 22 May 2023

(early access)

Published 18 October 2024

Authors’ Contribution

WE and SAA designed the study. OT collected the samples. SAA and OT conducted experimental work. WE analyzed the data. All authors wrote, reviewed and agreed to publish the manuscript.

Key words

Heterophyids, Fish, Histopathology, PCR, Attitude and practices

DOI: https://dx.doi.org/10.17582/journal.pjz/20220802120836

* Corresponding author: walid.elmonir@gmail.com

0030-9923/2024/0006-2755 $ 9.00/0

Copyright 2024 by the authors. Licensee Zoological Society of Pakistan.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

INTRODUCTION

Fish is a beneficial diet option and an important source of food and jobs, particularly in developing countries (Ridha, 2006). Heterophyids trematodes are large group of minute flukes with least 36 genera infecting animals, birds and humans (Masala et al., 2016; Chai and Jung, 2017). Fish-borne zoonotic heterophyids infecting humans constitute 13 genera and 29 species worldwide. More than 30 million people are estimated to be infected with these zoonotic trematodes globally (Chai and Jung, 2017). Their life cycle involves snail and fish as the first and second intermediate hosts, respectively (Masala et al., 2016). Mullet fish comes first at the list of the most preferred second intermediate hosts of heterophyids (Scholz, 1999) which could detrimentally harm the fish and in the same time constitute a zoonotic threat for fish consumers (Chai and Jung, 2017; Mahdy et al., 2020). Undercooked or raw fish dishes are the main sources of human infection of fish borne heterophyids (Chai and Jung, 2017; Khoa et al., 2020). Heterophyiasis in human is usually accompanied with gastrointestinal disturbances; however, ectopic infections beyond the intestine do occur and most commonly involve the brain, spinal cord, and heart (Belizario Jr et al., 2001).

In Egypt, heterophyiasis is a highly endemic yet under-recognized public health issue (Youssef and Uga, 2014). The disease in humans was reported at rates of 13.3% to 33.8% in several locations in northern Egypt (Abou-Basha et al., 2000; Lobna et al., 2010). Similarly, the heterophyids encysted metacercariae (EMC) were detected at relatively high rates (23%-42%) in fresh and brackish water fish in several locations especially the major lakes as Burullus and Manzala lakes in northern Egypt (Lobna et al., 2010; El-Sayad et al., 2014). Previous reports showed that zoonotic transmission of fish-borne parasitoses may be influenced by traditional food habits, like consuming raw, improperly smoked, salted or cooked fish (Khoa et al., 2020) and level of public knowledge about these zoonoses (Tegen and Damtie, 2021). However, there no sufficient data about knowledge or risk practices among residents in Egypt especially those at risk in northern governorates. Additionally, histopathological characteristics associated with EMC infection in fish and using of molecular techniques for detection and discrimination of heterophyids EMC in fish are scanty in Egypt. Therefore, this study aimed to record the prevalence of heterophyids EMC in brackish water fish (mullet) and fresh water fish (tilapia) using histopathological and molecular techniques. Also, to investigate the knowledge, attitude and practices related to fish-borne parasitic zoonoses among residents in Kafrelsheikh Governorate, Egypt.

MATERIALS AND METHODS

Fish sampling

A total of 50 tilapia (Oreochromis niloticus) and 50 mullet (Mugil cephalus), were randomly collected from local markets in Kafrelsheikh Governorate during the period between October 2016 and January 2017. Tilapia fish had a body weight of 165 - 308 g and a length of 18 - 25 cm, while mullet fish had a body weight of 152 - 298 g and a length of 22 - 28 cm. All examined fish were fresh and passed the organoleptic examination.

Histopathological detection of heterophyids EMC

A total of 100 fish specimens were collected, 50 of which belonged to Tilapia nilotica and the other 50 to Mugil cephalus. Tissues sections from muscle were taken and rapidly fixed in 10% neutral buffered formalin solution. Fixed specimens were dehydrated in ascending grades of ethanol, cleared in xylene, and embedded in paraffin wax. 4-µm-thick paraffin tissue sections were obtained and stained with hematoxylin and eosin (H and E) (Bancroft and Layton, 2013) and examined microscopically to detect any EMCs and their associated histological alterations.

Molecular detection of heterophyids EMCs

The EMC DNA was extracted from muscles sample of each fish using a DNeasy tissue kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions. All DNA extracts were quantified by NanoDrop 2000 (Thermo Fisher Scientific, Delaware, USA) and stored at −20 °C till further analysis.

For PCR detection of EMC belonged to Heterophyidae family, the 18S rDNA was targeted as previously described (Dzikowski et al., 2004). The PCR mixture consisted of 5 μL of DNA template (~50 ng), 12.5 μL of EmeraldAmp MAX PCR Master Mix (Takara Bio, Kusatsu, Japan), 1 μL (20 pmol) of each primer (Table I), and distilled water up to a final volume of 25 μL. The PCR cycling conditions were as follow: 94°C for 7 min followed by 35 cycles of [94°C for 1 min, 53°C for 1 min, and 72°C for 1 min], and a final extension at 72°C for 10 min. The PCR was conducted in an Applied Biosystem 2720 thermal cycler (Applied Biosystems, Foster City, CA, USA). The DNA of Heterophyes heterophyes was used as positive control and sterile distilled water was a negative control. The PCR products (361 bp) were visualized with an Alpha Imager (Alpha Innotech, San Leandro, CA, USA).

The EMC belonged to genus Heterophyes was detected by targeting genus-specific cytochrome c oxidase 1 (COI) gene (Nouh et al., 2010). The primers used are listed in Table I. The PCR mixture and cycling conditions were the same as described for the18S rDNA.

Knowledge, attitude, and practices (KAPs) investigation regarding fish borne parasitic zoonoses

The KAPs regarding fish borne parasitoses were evaluated using a questionnaire. The questionnaire was pretested using a small group of residents of Kafr-Elsheikh city and then a total of 100 residents of various ages, professions, and educational levels were interviewed using the pre-tested questionnaire. The questionnaire contained questions regarding knowledge of fish-borne parasitoses and their related practices.

Statistical analysis

The odds ratio was calculated using the univariate logistic regression analysis in SPSS statistics software version 21.0. (IBM SPSS Inc., Armonk, NY, USA).

RESULTS

Prevalence of heterophyids EMC in examined fish

The histopathological examination showed that EMC were detected in 44% (22/50) and 20% (10/50) of examined mullet and tilapia fish samples, respectively (Table II) with an overall prevalence of 32% (32/100).

 

Table I. The primers used in this study.

Target gene	Oligonucleotide sequence (5′ → 3′)	Product size (bp)	Reference
18S rDNA	F: TCATATGCTTGTCTCAGA	361	Dzikowski et al. (2004)
	R: ACGGAAACCTTGTTACGA
COI	F: CGGAGGAAAAGAAACTAACC	117	Nouh et al. (2010)
	R: CGAGCCAACCTGAACACCAC

 

Table II. Prevalence and odds ratio of EMC in muscles of examined tilapia and mullet fish in this study.

Variables	EMC in Fish samples			Univariate logistic regression
	Mullet	Tilapia	Total	OR*	P value	C.I. 95%
EMCTotal	22/50 (44)	10/50 (20)	32/100 (32)	3.1	0.01**	1.3 - 7.5
EMCHeterophyidae	16/50 (32)	9/50 (18)	25/100 (25)	2.1	0.1	0.8 - 5.3
EMCHeterophyes	16/50 (32)	8/50 (16)	24/100 (24)	2.4	0.07	0.9 - 6.3

 

EMC, Encysted metacercariae; OR, Odds ratio; C.I., Confidence Interval; Brackets, Percent. *Tilapia is the reference category; **, significant at P <0.05.

 

The EMCs were 3 times more likely detected in mullet fish compared to tilapia fish (OR= 3.1, P= 0.01). The Heterophyids EMC (EMCHeterophyidae) were molecularly confirmed in 32% and 18% of mullet and tilapia fish samples, respectively (Table II, Fig. 1). All mullet EMCHeterophyidae belonged to genus Heterophyes (EMCHeterophyes; 32%), while EMCHeterophyes were detected in 16% of tilapia fish samples (Table II, Fig. 1). The EMCHeterophyidae and EMCHeterophyes were more likely associated with mullet than tilapia fish samples (OR= 2.1 – 2.4); however, these differences were not significant (P= 0.07 - 0.1).

 

Histopathological changes associated with heterophyids EMCs

Histopathological examination of the fish muscles revealed that heterophyids EMCs were commonly seen embedded in intermuscular fat of mullet fish (Fig. 2A) and between the muscle bundles in tilapia fish with scanty tissue reaction (Fig. 2B). The presence of EMCs was associated with hyalinization of muscle bundles, mononuclear cells infiltration and many melanophores (Fig. 2C). Furthermore, some regions showed rarefication of necrosed muscle bundles (Fig. 2D), as well as atrophy in some muscle bundles (Fig. 2E). In tilapia, the above-mentioned changes were less pronounced than in mullet (Fig. 2F).

KAPs regarding fish-borne parasitic zoonoses among residents of Kafr Elsheikh city

The results of KAPs survey revealed that 50% (50/100) of the respondents were aware of fish-borne parasitic zoonoses and only 14% of them (7/50) had knowledge about heterophyiasis (Table III). However, 98% (49/50) knew that fish consumption was the mode of fish-borne parasitoses transmission to humans (Table III). More than half of respondents (56.4%) consume fish meals multiple times per week. Majority of residents prefer eating fish fried or grilled (61.9% – 85.6%), and around third (32.9%) of them eat salted fish (Table III). Few residents (2.3%) cooked fish for less than 10 min, and half of those who salted fish at home keep their fish in salt for less than 10 days (Table III). Majority of the respondents (95.8%) prefer to purchase fresh fish rather than frozen ones and most of them (92.7%) consume their fish, without freezing, at same day of purchase (Table III).

 

Table III. KAPs related to fish-borne parasitic zoonoses among residents in the study area.

Questions	Categories	R	A	P
Identification
	Gender
	Male	36	-	36%
	Female	64	-	64%
	Age
	20 years >	3	-	3%
	20:40 years	57	-	57%
	40 years <	40	-	40%
Questions	Categories	R	A	P
	Education
	No	15	-	15%
	Medium	27	-	27%
	High	58	-	58%
	Profession
	Fish related	16	-	16%
	Agriculture related	3	-	3%
	Veterinarian	5	-	5%
	Medical related	13	-	13%
	Others	63	-	63%
Knowledge
	Knowledge of diseases transmitted via fish
	Yes	100	50	50
	No		50	50
	Types of diseases transmitted via fish
	GIT disturbances	50	19	38
	Heterophyiasis		7	14
	Other diseases		24	48
	Modes of these diseases transmission from fish to human
	Ingestion	50	49	98
	Contact		10	20
Practices
	Rate of fish eating
	Once per week	94	38	40.4
	˃ Once per week		56	59.6
	Preferred type of fish processing
	Fried	97	60	61.9
	Grilled		83	85.6
	Salted		32	32.9
	Smoked		31	31.9
	Preferred cooking time
	>10 min.	86	2	2.3
	10:15 min.		48	55.8
	<15 min.		36	41.9
	Preferred time for fish salting
	>10 days	8	4	50
	≥10days		4	50
	Preferred fish for purchase
	Fresh	96	92	95.8
	Frozen		9	9.4
	Fish freezing after purchasing
	Yes	96	19	19.8
	No		89	92.7

 

R, Respondents; A, Answers; P, Percent.

 

DISCUSSION

Kafrelsheikh governorate contributes by about fifth of the total Egyptian fish production (Elzohry et al., 2020) and fish is one of the preferable food sources for the governorate residents. Additionally, the high rate of heterophyids infection in Egypt were reported among human residents and stray animals of this governorate (Youssef and Uga, 2014; Chai and Jung, 2017). Hence, fish-borne zoonoses are of concern in this governorate.

In this study, the overall prevalence rate of fish EMCs was 32%. Molecular testing confirmed heterophyids EMCs in 25% of examined fish. This was comparable with a previous report (23%) in Alexandria, Egypt (El-Sayad et al., 2014), yet lower than that reported (32%) in Nile delta of Egypt (Lobna et al., 2010). The total EMCs and heterophyids EMCs were more likely detected in mullet fish (44% and 32%) than tilapia fish (20% and 18%), respectively. In agreement, mullet fish was reported as the most preferred second intermediate hosts of heterophyids (Scholz, 1999; Chai and Jung, 2017). However, (Lobna et al., 2010) reported higher rates of heterophyids EMCs in tilapia than mullet fish. This variation in fish infection rates between studies may be linked to variations in conditions that support the parasite life cycle, such as differences in habitat, food supply, and the abundance of both aquatic snails (the intermediate host). For instance, it was previously reported that fish grew in natural surface water are more prone to Heterophyids infection than those in aquacultures, regardless of fish species (El-Sayad et al., 2014). This was attributed to less number of snails and the use of prophylaxis chemotherapy which reduce incidence of fish infection in aquaculture (El-Sayad et al., 2014). Also, variations in temperature and humidity from season to another can affect snails’ reproduction, and hence the infection rate of fish (El-Leathy, 1997).

Routine microscopic and histopathological identification of heterophyids EMCs at family or genus levels are not applicable due to similarity of morphology between EMC of trematode infections (Chai and Jung, 2017). Moreover, the use of in vivo experimental infection for heterophyids genus identification (e.g. feeding EMCs to puppies) is laborious and time consuming. In this study, molecular testing identified heterophyids EMCs at family (Heterophyidae) level using the 18S rDNA and genus (Heterophyes) level using the COI gene. Molecular testing is still in developing stage for detection of heterophyids yet it is a promising tool that provides rapid, sensitive and reliable method for heterophyids identification (Dzikowski et al., 2004; Chai and Jung, 2017). Several studies reported use of 18S rDNA and the COI gene for heterophyids family and genera discrimination in fish worldwide (Dzikowski et al., 2004; Nouh et al., 2010; Chontananarth et al., 2014).

In the current study, histopathological evaluation of the collected samples revealed a more heavy infection with EMCs in mullet than tilapia fish, which were most typically detected in the intermuscular fatty tissue. In addition, mullet had more muscle atrophy and injury, as well as inflammatory cell infiltration than tilapia fish. Tissue reaction against EMC depends on infection period and the type of the metabolites induced by the parasitic cysts (Aly et al., 2005). In agreement with our findings, previous reports showed that heterophyids EMCs were abundant in the fatty areas and around the internal organs of infected fish (Jitra and Urusa, 2014; Mahdy et al., 2020). Some digenetic trematodes take up their requirements of the fatty acids from their host (Debasree and Misra, 2014). Therefore, the parasites’ need for some important fatty acids from the host, and the high fat content of mullet fish compared to other fish species (Gabr and Ali, 2007), could clarify concentration of EMC in fatty areas and the higher rate of this parasite in mullets compared to tilapia in this study. The histopathogical changes associated with heterophyids EMC infection highlighting its potential harm effect on fish meat quality, especially in the mullet fish.

The KAPs survey in this study showed that 50% of the residents didn’t know about fish-borne parasitic zoonoses and majority of them had no knowledge about heterophyiasis. Lack of knowledge was previously reported as a risk factor for parasitic diseases among humans (Tegen and Damtie, 2021). More than half of Kafrelsheikh residents consume fish meals more than once per week. The high rate of fish consumption facilitates infection transmission (Ridha, 2006), and thus considered a predisposing risk for fish-borne zoonoses in the study area. Majority of residents prefer eating fish grilled (85.6%) especially mullet fish. A previous report in Egypt showed that 20-30% of the mullet fish retained viable heterophyids EMC after grilling in a way similar to Egyptian fishermen grilling (Hamed and Elias, 1970). This indicates that insufficient heat treatment during grilling may pose a risk of fish Heterophyids transmission to consumers. Few residents (2.3%) cooked fish for less than 10 min, and 50% of them salted fish at home for less than 10 days. Inadequate cooking and/or salting time of fish were previously reported as risk factors for fish-borne heterophyids infection (Abdallah et al., 2009; Chai and Jung, 2017).

Majority of the residents (> 90%) prefer to purchase fresh fish and consume their fish at the same day purchase without freezing. Freezing was recommended by the WHO as an effective protective method against fish-born trematodes infection (WHO 1979). Also several studies reported loss of heterophyids EMC viability in fish after freezing foe 2 days to 2 weeks (Hamed and Elias, 1970; El-Sayad et al., 2014). This means that residents who consume fresh fish without freezing are prone to increased risk of heterophyids infection in study area.

conclusion

In conclusion, this study reported high rate of heterophyids EMC in mullet and tilapia fish in study area. The recorded EMC infection associated pathological lesions highlighted poor meat quality of infected fish. Lack of knowledge regarding heterophyiasis and several risk practices reported by majority of residents necessitate public awareness campaign. Also, measures should be taken for infection control among fish to insure high quality of fish and safety of consumers in study region.

Statement of conflict of interest

The authors have declared no conflict of interests.

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