Identification of Secondary Metabolites from Callus Orthosiphon aristatus (Blume) Miq by Thin Layer Chromatography
Identification of Secondary Metabolites from Callus Orthosiphon aristatus (Blume) Miq by Thin Layer Chromatography
Fahrauk Faramayuda1,2*, Totik Sri Mariani3, Elfahmi1,4 and Sukrasno1
Figure 1:
Callus O. aristatus purple varieties. (a) MS media, (b) Gamborg media, (c) N6 media and (d) SH media.
Figure 2:
Callus O. aristatus white-purple varieties. (a) MS medium, (b) Gamborg media, (c) N6 media, and (d) SH media.
Figure 3:
TLC profile of purple variety callus extract. Stationary phase: Silica GF254 Mobile phase: Toluene: Ethyl Acetate: Formic Acid: Water (3: 3: 1: 0.2).
S: Sinensetin; AR: Rosmarinic Acid; E: Eupatorin; MU1: Callus purple varieties ethanol extract. (MS medium); MU2: Callus purple varieties ethyl acetate extract. purple (MS medium); MU3: Callus purple varieties acetone extract. (MS medium); MU4: Callus purple varieties n-hexane extract (MS medium); GU1: Callus purple varieties ethanol extract (Gamborg medium); GU2: Callus purple varieties ethyl acetate extract. purple (Gamborg medium); GU3: Callus purple varieties acetone extract. (Gamborg medium); GU4: Callus purple varieties n-hexane extract (Gamborg medium); NU1: Callus purple varieties ethanol extract (N6 medium); NU2: Callus purple varieties ethyl acetate extract. purple (N6 medium); NU3: Callus purple varieties Acetone extract (N6 medium); NU4: Callus purple varieties n-hexane extract (N6 medium); SU1: Callus purple varieties ethanol extract (SH medium); SU2: Callus purple varieties ethyl acetate extract. purple (SH medium); SU3: Callus purple varieties acetone extract (SH medium); SU4: Callus purple varieties n-hexane extract (SH medium).
Figure 4:
TLC profile of callus extract from white-purple variety. Stationary phase: Silica GF254 Mobile phase: Toluene: Ethyl Acetate: Formic Acid: Water (3: 3: 1: 0.2).
MP1: Callus white-purple varieties ethanol extract. (MS medium); MP2: Callus white-purple ethyl acetate extract. purple (MS medium); MP3: Callus white-purple acetone extract. (MS medium); MP4: Callus white-purple n-hexane extract (MS medium); GP1: Callus white-purple ethanol extract (Gamborg medium); GP2: Callus white-purple ethyl acetate extract. purple (Gamborg medium); GP3: Callus white-purple acetone extract. (Gamborg medium); GP4: Callus white-purple n-hexane extract (Gamborg medium); NP1: Callus white-purple ethanol extract (N6 medium); NP2: Callus white-purple ethyl acetate extract. purple (N6 medium); NP3: Callus white-purple acetone extract (N6 medium); NP4: Callus white-purple n-hexane extract (N6 medium); SP1: Callus white-purple ethanol extract (SH medium); SP2: Callus white-purple ethyl acetate extract. purple (SH medium); SP3: Callus white-purple acetone extract (SH medium); SP4: Callus white-purple n-hexane extract (SH medium).
Figure 5:
TLC profile of wild type extract and callus varieties of purple. Stationary phase: Silica GF254 Mobile phase: Toluene: Ethyl Acetate: Formic Acid: Water (3: 3: 1: 0.2).
DU1: leaf ethanol extract of purple varieties (wild type); BU1: stem ethanol extract of purple varieties (wild type); DU2: ethyl acetate extract of purple varieties (wild type); BU2: stem ethyl acetate extract of purple varieties (wild type); DU3: acetone extract of purple varieties (wild type); BU3: stem acetone extract of purple varieties (wild type).
Figure 6:
TLC profile of wild type extract and callus white-purple varieties. Stationary phase: Silica GF254 Mobile phase: Toluene: Ethyl Acetate: Formic Acid: Water (3: 3: 1: 0.2).
DP1: leaf ethanol extract of white-purple varieties (wild type); BP1: stem ethanol extract of white-purple varieties (wild type); DP2: ethyl acetate extract of white-purple varieties (wild type); BP2: stem ethyl acetate extract of white-purple varieties (wild type); DP3: acetone extract of white-purple varieties (wild type); BP3: stem acetone extract of white-purple varieties (wild type).
September 2021
Vol. 37, Iss. 3, Pages 714-1097
