Submit or Track your Manuscript LOG-IN

Identification and Characterization of Salmonella Enteritidis and Salmonella Typhimurium in Table Eggs In Peshawar, Pakistan

Identification and Characterization of Salmonella Enteritidis and Salmonella Typhimurium in Table Eggs In Peshawar, Pakistan

Imtiaz Ali Shah1*, Maleeha Anwar1, Rafiullah2, Inamullah Wazir1, Muhammad Hasnain Riaz1, Abdur Raziq1, Muhammad Ijaz Ali1, Faizul Hassan1, Khalid Khan3, Yasin Ahmad4, Irshad Ahmad5, Muhammad Ibrahim Rashid5, Muhammad Tariq Zeb1* 

1Veterinary Research Institute, Khyber Pakhtunkhwa Peshawar, Pakistan; 2Veterinary Research & Disease Investigation Center, Kohat, Pakistan; 3Foot & Mouth Disease Vaccine Research Center, Peshawar, Pakistan; 4 Institute of Biological Sciences, Sarhad University of Science & Information Technology, Peshawar; 5Institute of Basic Medical Sciences, Khyber Medical University, Peshawar.

*Correspondence | Imtiaz Ali Shah, Muhammad Tariq Zeb, Veterinary Research Institute, Khyber Pakhtunkhwa Peshawar, Pakistan; Email: drimtiazvri@gmail.com, drtariqzeb@gmail.com 

ABSTRACT

In the present study, we investigated the prevalence of Salmonella in various parts of table eggs collected from various union councils of Peshawar, Khyber Pakhtunkhwa Pakistan. For this purpose, 200 egg samples were collected. The culturing of Salmonella from egg shell and their contents was carried out according to ISO guidelines for Salmonella isolation. A total of twenty-two (22) eggs were positive for Salmonella species. The antibiotic susceptibility testing revealed that Salmonella isolates were resistant to ampicillin, amoxicillin/clavulanic acid, cefotaxime, and kanamycin while isolates were susceptible to chloramphenicol, gentamicin, kanamycin and streptomycin. These positive samples were further investigated and confirmed through PCR by targeting serovars specific genes i.e rfbJ, fliC, fljB for S. Typhimurium and ST11, SPV, SefA for S. Enteritidis. The prevalence of Salmonella was found to be 22 (11%), out of which 17 (77.27%) isolates were S. Enteritidis and 5 (22.73%) isolates were found to be S. Typhimurium as confirmed through PCR. The presence of infectious Salmonella in table eggs in Peshawar presents a serious public health threat and should be monitored on routine basis for the presence of Salmonella.

 

Keywords: Table eggs, PCR, Public Health, Salmonella. 

To share on other social networks, click on any share button. What are these?

South Asian Journal of Life Sciences

December

S. Asian J. Life Sci., Vol. 12

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe