Identification and Functional Analysis of miRNAs in PEDV-Infected PK-15 Cells
Identification and Functional Analysis of miRNAs in PEDV-Infected PK-15 Cells
Miao Yin1,2,3,4, Xi-Wen Chen1,2,3,4, Zuo-Jie Xie1,2, Cong Wang1,2, Zhi-Chao Song1,2 and Jun-Qiao Chen1,2
ABSTRACT
The aim of this experiment was to study the dynamic changes of PK-15 miRNA in porcine kidney passage cells infected by porcine epidemic diarrhea virus (PEDV). We collected samples from PK-15 cells at different time points after PEDV infection, analysed the changes in the course of PEDV infection, extracted total RNA and constructed libraries, performed high throughput sequencing, established miRNA expression profiles of PK-15 cells in control and infected groups and performed differential analysis, and performed Miranda prediction and GO (Gene Ontology, GO) functional analysis for mRNAs with significant differences. GO functional enrichment analysis was performed for the significantly different mRNAs. The results showed that PEDV reached peak viral load on the second day after infection with PK-15 cells, and 280 differentially expressed mRNAs were found after sequencing, among which 96 mRNAs were up-regulated and 184 mRNAs were down-regulated, among which ssc-miR-32 expression was significantly up-regulated. Prediction of ssc-miR-32 showed that it may regulate leucine zipper protein 1 (LUZP1), and GO analysis showed that LUZP1 mainly affects the formation and development of nerve, epithelium, heart and embryo, suggesting that ssc-miR-32 may mainly affect virus replication by regulating the biological process of cells. This study provides a scientific basis for miRNA regulation of the pathogenic mechanism of PEDV porcine epidemic diarrhoea virus.
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