Identification of A Virus From Naturally Infected Garlic Plants
Identification of A Virus From Naturally Infected Garlic Plants
S.A. El-Kewey1; R.A. Omar1; S.A. Sidaros2 and Samaa Abd El-Khalik3
ABSTRACT
The isolated virus was identified according to host range, symptomatology, differential hosts, virus stability, modes of transmission, and electron microscopy. The virus has a narrow host range. Dilution end point was found to be 10-2. Thermal inactivation point was determined as 65oC, and infectivity was retained for one day only. The virus transmitted mechanically, and by means of Myzus persicue and Aphis fabue. Green peach aphid was found to be more effective in virus transmission than broad bean aphid. Virus transmission through vegetative propagation reached 100% in both cultivars (Chinese and Balady) with different degrees of symptoms severity. Plant height, leaf area. fresh and dry weight of bulb decreased significantly with the increasing of severity symptoms on garlic plants of both cultivars. The electron microscope preparations of crude sap extracted from naturally infected garlic plants and negatively stained with 2% PTA showed flexuous rod-shaped particles 657-714 nm long. Biological relationships between the virus and insect vectors were studied.
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