Identification, characterization and ultrastructure aspects of Alfalfa mosaic virus infecting potato (Solanum tuberosum L.) in Egypt
Identification, characterization and ultrastructure aspects of Alfalfa mosaic virus infecting potato (Solanum tuberosum L.) in Egypt
Maha A. El-Abhar1, Moustafa A. Elkady1, Khaled M. Ghanem2, Hussieny A. Bosila3
ABSTRACT
Background: Alfalfa mosaic virus (AMV) is only virus in the genus Alfamovirus and has very wide host
range among weed and crop plants which produces a variety of symptoms. It can cause problems in potato
in some regions where vectors easily move into potato fields from reservoir host, particularly if a tuber
necrosis-causing strain is involved.
Objective: : The purpose of this study is to characterize biologically and serologically AMV infecting
potato (Solanum tuberosum L.) in Egypt. Moreover, the study described the histological and cytological
effects of AMV infection in potato leaf cells.
Methods: Leaf samples were collected on the basis of visual symptoms from potato plants with yellow
blotching symptoms, called “Calico” and leaf distortion. A sap-transmitted virus isolated from potato
was biologically purified after three successive single local lesion passages onto Chenopodium
ammaranticolor which reproduced prominent local lesions. The virus isolate was then propagated in
potato Ditta cv. plants, The virus was identified on the bases of host range, symptomatology,
transmission and serological diagnosis, in addition to the ultrastructural changes produced in potato
leaf cells infected with AMV .
Results: Reaction of thirteen plant species and cultivars belonging to four families (Amaranthaceae,
Solanaceae, Fabaceae and Laminaceae) to AMV infection was demonstrated. The presence or
absence of the virus was verified by back inoculation onto healthy indicator host plant and/or ELISA
test. AMV was readily transmitted by mechanical means and by Myzus persicae with percentage of
60 %. In addition to visible symptoms, infection with AMV also causes ultrastructural changes in
potato leaf cells. Examination of epidermal strips of N. tabacum cv. White Burley using light
microscope showed amorphous cytoplasmic inclusion bodies seemed to be attached to the nucleus
from one or two sites, while those inclusions have never been observed in the epidermal stripes of
healthy leaves. Electron microscopy, revealed cytological and histological changes induced by Alfalfa
mosaic virus infection in potato leaves
Conclusion: In this work, Alfalfa mosaic virus (AMV) characterized and ultrastructure aspected of
infecting potato (Solanum tuberosum L. ) in Egypt illustrating important effect of AMV on potato
plant. Finally our recommendions to use a ground zeolite alone as a potential and highly economic
adjuvant in FMD vaccine for cattle.
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January 2018
Vol. 3, Iss. 1, Pages 1-77
