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In-Vitro Propagation for Conserving the Gems of the Hills Caralluma tuberculata to Rescue the Valuable Endangered Medicinally Important Plant

In-Vitro Propagation for Conserving the Gems of the Hills Caralluma tuberculata to Rescue the Valuable Endangered Medicinally Important Plant

Muhammad Taqi1, Shazia Erum2, Shamaila Rasheed2, Sadar Uddin Siddiqui2 and Shakeel Ahmad Jatoi2*

1Department of horticulture, University of Agriculture, Peshawar, Pakistan; 2Bio Resources Conservation Institute, NARC, Islamabad, Pakistan.

 
Shakeel Ahmad Jatoi, Bio Resources Conservation Institute, NARC, Islamabad, Pakistan; Email: [email protected]

Figure 2:

Morphological characteristics of callus observed during in-vitro propagation of Caralluma tuberculate against different PGR supplemented to MS-medium.

Figure 3:

Shoot induction in Carraluma tuberculata against different concentration of different plant growth regulators; A) BAP mg/L, B) Kin mg/L. C) GA3 mg/L, D) BAP+KIN mg/L, E) BAP+NAA+Kin mg/L.

Figure 4:

Number of shoots and shoot length (cm) in Carraluma tuberculata against different concentration of different plant growth regulators; A) BAP mg/L, B) Kin mg/L, C) GA3 mg/L, D) BAP+KIN mg/L, E) BAP+NAA+Kin mg/L.

Figure 5:

Root induction in Carraluma tuberculata against different concentration of A) IBA+NAA, B) NAA+IBA.

Figure 6:

Number of roots and root length recorded in Carraluma tuberculate against different concentration of A) IBA+NAA, B) NAA+IBA.

Figure 7:

Different stages of multiple shoot formation of medium containing BAP +NAA +Kin (a and b), variation in shoot length of C tuberculata (b, c, e, f).

Figure 8:

Root morphology observed in Carraluma tuberculata; A) IBA (1 mg/L) +NAA 0.5 mg/L, B and D) (1.5 mg/L) +NAA 0.5 mg/L, C) NAA (1 mg/L) +IBA 0.5 mg/L, E) NAA (2 mg/L)+IBA 0.5 mg/L.

Figure 1:

Response of different concerntration of different plant growth regulators on callus induction in Carraluma tuberculata; A) 2,4-D alone, B) 2,4-D+BAP, C) 2,4-D+Kin, D) BAP+NAA+KIN.

Figure 1:

Response of different concerntration of different plant growth regulators on callus induction in Carraluma tuberculata; A) 2,4-D alone, B) 2,4-D+BAP, C) 2,4-D+Kin, D) BAP+NAA+KIN.

Pakistan Journal of Agricultural Research

September

Vol.37, Iss. 3, Pages 190-319

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