Inhibitory effect of cyanobacteria byproducts on Bean yellow mosaic virus on faba bean plant
Inhibitory effect of cyanobacteria byproducts on Bean yellow mosaic virus on faba bean plant
Radwa M. Shafie 1 and Soha S. M. Mostafa2
ABSTRACT
The inhibitory effect of culture filtrates and fresh biomass of three cyanobacteria isolates i.e., Nostoc muscorum, Spirulina platensis and Oscillatoria sp. was studied, individually or in a mixture, against Bean yellow mosaic virus (BYMV) infection on Vicia faba L. Giza 716 and Chenopodium amaranticolor Cost and Reyn. The non-inoculated algal synthetic media were used as control. Findings revealed that the mixture of the three algal filtrates gave the highest percentages of inhibition. Lower inhibition effect was produced by culture filtrate of N. muscorum and S. platensis, respectively in particular in plants treated with algal filtrates by mixed with virus inoculum immediately before inoculation. Spraying plants with the algal filtrates 24 hrs. before inoculation produced a higher inhibitory effect against BYMV than that obtained by spraying 24 hrs. after inoculation. The same trend of inhibition effect that was detected with algal biomass was lower than with filtrates. The indirect ELISA was carried out to confirm the identity of the virus isolate and the obtained results of this study. Spraying faba bean plants with either algal filtrates or biomass also increased the plant growth parameters, i.e. plant stem and root length as well as plant fresh and dry weight. Algal proteins were identified by the protein profile pattern using SDS-PAGE method. The contents of alkaloids, phenols and terpenoids in both algal filtrates and biomass were determined.
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