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L-Carnitine Supplementation During In vitro Maturation of Egyptian Buffalo Oocytes Improved Embryo Yield and Decreased Blastocyst Apoptotic Rate

L-Carnitine Supplementation During In vitro Maturation of Egyptian Buffalo Oocytes Improved Embryo Yield and Decreased Blastocyst Apoptotic Rate

Shorouq Mohamed Omara1, Ibrahim Saad El-Shamaa1, Emad Abd-Elaziz Abd-Allah2, Assmaa Abd-Allah Fathy3, Essam-Eldin Tharwat4, Mohammed Hamdy Farouk5* and Ibrahim Mahmoud Abd El-Razek1*

1Animal Production Department, Faculty of Agriculture, Kafrelshiekh, University, Kafrelsheikh, Egypt
2Biotechnology Research Department, Animal Production Research Institute, Agriculture Research Center, Egypt
3Animal Production Department, Faculty of Agriculture, Menoufia University, Menoufia, Egypt
4Animal Production Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
5Animal Production Department, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo, Egypt
 
*      Corresponding author: mhfarouk@jlau.edu.cn, ibrahimabdelrazek2006@yahoo.com

Fig. 1.

Nuclear maturation. (A) Fresh oocyte where first polar body was extruded in previtilline space (metaphase II). (B) Oocyte stained by orcein stain and showed that chromosomes at metaphase II.

Fig. 2.

Morula stage embryo. The formed embryos were examined using stereomicroscope after seven days of culture by inverted microscope for kinetic cleavage stage (blastocyst stages).

Pakistan Journal of Zoology

August

Pakistan J. Zool., Vol. 56, Iss. 4, pp. 1501-2000

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