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Label-Free Quantification based Proteomic Analysis of Serum Obtained from Henoch–Schönlein Purpura Patients before and after Zhenbao Pill Treatment

Label-Free Quantification based Proteomic Analysis of Serum Obtained from Henoch–Schönlein Purpura Patients before and after Zhenbao Pill Treatment

Wuritunashun*, Burenbatu, Narenqiqige, Jiuguniang, Eerdunduleng, Shuanglian Wang, Cuiqin Gong, Hashengaowa, Huizhi Jin, Baiwurihan and Chunhaizi

Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, China

 
* Corresponding author: nx15313295064@163.com

Fig. 1.
Flow chart of the experimental scheme. HSP patients were treated with Zhenbao pill, samples were collected and processed. A label-free quantification proteomics was performed and data was analyzed with bioinformatics tools. Proteins were validated by PRM.
Fig. 2.

(A) Summary of the total number of GO hits. (B) Total number of upregulated and downregulated proteins using LFQ analysis.

Fig. 3.

Biological processes involving the differentially expressed proteins. Each bar includes the number of genes and p-value calculated with Fish exact test with Hypergeometric algorithm. Max level means maximal annotated level of this term in the GO graph. 

Fig. 4.

(A) Cellular components, (B) KEGG pathways involving the differentially expressed proteins.

Fig. 5.

Protein protein interactions network of the differentially expressed proteins.

Fig. 6.

Validation of differentially expressed proteins (DEPs) using parallel reaction monitoring analysis. Two candidate DEPs were used to verify the LFQ proteomics results.

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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