Mitochondrial Cytochrome b based Identification of Some Commercially Important Fishes Found in the Arabian Sea Coast of Pakistan
Mitochondrial Cytochrome b based Identification of Some Commercially Important Fishes Found in the Arabian Sea Coast of Pakistan
Adil Shehzad, Obaid Yusuf Khan and Maqsood Ali Ansari*
ABSTRACT
Fish industry is serving as a backbone of the economy of many countries. In order to gain high and easy profit, commercial frauds of mislabeling has been reported earlier in many different cases. Chances of mislabeling a relatively less expensive fish with an expensive one particularly if they are morphologically similar gets very high as it is not easy to differentiate them. This problem increases when it is the case of processed fish as morphological characters are not available for the identification. DNA based methods are likely to solve this dilema. To our knowledge no report has been made on the DNA based methods of marine fish identification though there are some reports of DNA based fresh water fish identification. We have used the cytochrome b gene of mitochondria for performing DNA sequencing and PCR-RFLP of 22 fish species belonging to different families of commercial importance and have shown that these fish can be successfully identified by these techniques.
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