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Molecular Characterization of Potato Spindle Tuber Viroid Egyptian Isolate

Molecular Characterization of Potato Spindle Tuber Viroid Egyptian Isolate

E. K. Allam1; Kh. A. El-Dougdoug1; M. A. Amer2; A. A. Abou-Zaid2; and S. M. Amin2

1 Microbiology Department (Virology lab.), Faculty of Agriculture Ain Shams University, Shubra El-Kheima, Cairo, Egypt. 
2 Virus and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.

ABSTRACT

Potato spindle tuber viroid (PSTVd) was detected in potato plants grown in Egypt using return-polyacrylamide gel electrophoresis (R-PAGE) and molecular techniques. The method involved the extraction of circular nucleic acids followed by electrophoretic analysis of the extracts on 5% polyacrylamide gel. A reverse transcription-polymerase Chain reaction (RT-PCR) assay was used for the isolation and identification of PSTVd cDNA from infected plant materials using a specific primer to PSTVd. A major cDNA product of approximately 360 bp was successfully hybridized with digoxigenin labeled PSTVd cDNA probe.

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Journal of Virological Sciences

July

Vol. 3, Iss. 1

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